| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,940,584 | C→A | R351L (CGT→CTT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,940,584 | 0 | C | A | 97.4% | 101.2 / NA | 39 | R351L (CGT→CTT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (19/19); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGA > NC_000913/1940444‑1940721 | cTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGATTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATCTAACGCGTGGTGCAGCTACGACCATGAAGa > 2:613043/1‑143 (MQ=255) ttCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATgaagaa < 1:934279/143‑1 (MQ=255) ggcggggTGCGGGTGGGGCCGGGTTGAGCGTGCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATgaagaa < 2:964682/137‑1 (MQ=255) cacagcgCAGATGCGGCCCGCGTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATa < 2:527538/137‑1 (MQ=255) tAGTGCAGATGCGGCGCGGTGGAACGTACGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCGTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGc < 1:186385/143‑1 (MQ=255) gCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGGTTCACCTTCTGTCCCGGTTTCACCAGCATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAGGAAGGATAGCcaca > 2:1135184/1‑143 (MQ=255) cAGATGCGGCCCGGTTGAACGTCAGGTATTAAGGCAAAACGCGAGACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAACCTTGCGCAAGTGCATATAACGCGTGGAGTAGCTGCGACCATGAACAATAGCcacac > 2:84395/1‑142 (MQ=255) gATGCGGCGTTGTTGACCGTCCTGTCTTCCCGGACGCCTCGATCCGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGCGTCTGTCGCCAGCGCATATAACGCTTGGTGTAGCTGCGACCATGAAGAATAGCCACataa < 2:982078/143‑1 (MQ=255) gCCCGGTTGAACGTCCGGTATTACCGGAAGGCGCGGGACGGTCGCCACGTTTCACCTTCTGTCCCGGGTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAACTGCGACCAGGACGCATAGCCAGGTAATAActt > 2:139829/1‑141 (MQ=255) ggTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACctgctg < 1:915200/143‑1 (MQ=255) tttgccGGAATGCGCGATACGGGCGCCACGTTTCACCTTCTGTGCCGGTTTCACCAGAATCTTGCGCAAGTGCATCTCACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTgg < 2:836591/139‑1 (MQ=255) ttACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAGCCTGCTGCGCCACTGCGTGTggg > 2:541301/1‑142 (MQ=255) aCCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGGAGAATAGCCACATAATGACCTGCTGCGCCACTGCGTTTGGCaa > 1:939750/1‑143 (MQ=255) ggAAAGCGCGATACGGTCGCCACGTTTAACCATCTGTCCCGGGTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGATGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCAGCTGCGTCTGGCAacca > 2:22999/1‑143 (MQ=255) gAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATCGCCATCTCCTAACCGGAAGCAACACTGCGATTGGAAaccac > 1:305938/1‑143 (MQ=255) cgATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCa < 1:930912/143‑1 (MQ=255) cgATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCa < 1:907273/143‑1 (MQ=255) gATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACAAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACCGGGTTTGGCAACCACCACtttgc > 1:502602/1‑140 (MQ=255) ggTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCa < 2:280357/143‑1 (MQ=255) cGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACCTAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCAccc > 2:74463/1‑143 (MQ=255) tCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGc < 1:84395/143‑1 (MQ=255) tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCCGATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCCTCACCCACTGAAAGCACTGGCGTAc > 1:498530/1‑143 (MQ=255) ccGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATCCCCTGCTGCGCCTCTGCGTCTGGCAAACACAAACTCACCGTCACCCACTAAAAACAATGGCATAccc > 1:284124/1‑143 (MQ=255) ccGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCCCCACTTCACCGTCACCCACTGAAAGCACTGGCCTAccc > 1:690185/1‑143 (MQ=255) gTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATGAAAACCTGCTGCGCCCCTGCGGATGGCACCCACCCCTGCCCCGCAACCCACCGAAAGCACTGGCGCACCCTGc > 2:338556/1‑143 (MQ=255) ttCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCgg < 1:253424/143‑1 (MQ=255) gtCACCGGAAGCGTGCGCAAGTGCATATCACGCGGGGTGTAGCTGCGACCATGAAGTAGAGCCACACAATATCCTGCTGCGCCACTGCGTTTTGCAGCCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCgg < 2:934822/142‑1 (MQ=255) cttaCCATAAGCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTACGTCCATGAATAATAGCCACATAATAACCTTCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCgg < 2:190005/140‑1 (MQ=255) cAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCAGTGCGTTTGGCGACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATgg > 1:870544/1‑143 (MQ=255) aTCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGaa < 1:448286/143‑1 (MQ=255) tCTGGCGCAAGTGCATATAACGCGTGGGGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGaaa < 1:469711/143‑1 (MQ=255) cAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTCAAAGCACTGGCGTACCCTGCGGCACGGCGAAACCAacac > 2:292067/1‑143 (MQ=255) cAAGTGCAGAGAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAacac < 1:588761/143‑1 (MQ=255) gTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACctc > 1:621332/1‑143 (MQ=255) aatatAACGCTTGGTGTAGCTGCGACCATGAAGAATAGCCACATACTAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCTCCGTCACCCACTGAACGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCtgt < 2:974725/142‑1 (MQ=255) ataACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCCCTGAAAGCACTTGCGTACCCTGCGGCATGGCGAAATCAACTCCTCtgtgtg > 1:723218/1‑143 (MQ=255) taACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTgtgg < 1:340566/143‑1 (MQ=255) ggtgTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAAcgc > 1:19628/1‑143 (MQ=255) tAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGAcc < 1:832040/143‑1 (MQ=255) gACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAcc > 2:581492/1‑143 (MQ=255) cATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGa < 1:358746/143‑1 (MQ=255) | CTTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGA > NC_000913/1940444‑1940721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |