| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,941,326 | C→T | D104N (GAT→AAT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,941,326 | 0 | C | T | 98.2% | 166.9 / NA | 56 | D104N (GAT→AAT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (26/28); minor base G (1/0); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGCGAACGAATTT > NC_000913/1941194‑1941463 | cGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGaa < 1:1141466/143‑1 (MQ=255)cGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGaa > 1:653158/1‑143 (MQ=255) ccGTCCGCGGTTAATGTTCAGGAGAGTTGTTGACCGCTTTTAAGGTGATGCAATTCTTAGTACAACCCTGTCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGTTTGAGAATGCTGCTTAGCGTATTGCCAGTGGAaac < 1:312209/143‑1 (MQ=255) cgcgGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACat > 2:465529/1‑143 (MQ=255) gcgcTTAAGGTCCATGAGAGTGGTTGACCGATGTTCAGGTTACTCAATTCGTTGTCTGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCAGACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACata < 2:1055249/143‑1 (MQ=255) gcgGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCATAGCGCATTGGCATTGGAAACAACcta > 2:680035/1‑143 (MQ=255) gcgGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGAAGACT‑ATATGTACCCGTTTCAATACCAGGCTGATTGAGAATGCTCCTGAGCGTATGGCCCGTGGAAACAACata > 2:1115401/1‑143 (MQ=255) ttAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATAGCCAGTGGAAACAACATAttac > 2:148218/1‑141 (MQ=255) ttAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCAGACTGACTGAGAATGCGGCTTAGCGTATTGCCAGTGGAAAACACATATGCa > 2:809854/1‑143 (MQ=255) tAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑GACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCGGTGGAAACAACCTATGCAt > 1:234160/1‑143 (MQ=255) tAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCAt > 1:490597/1‑143 (MQ=255) ggtcacgaggGTTGTGGACCGATGTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCAc < 2:1091818/137‑1 (MQ=255) gTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCGGTGGAAACAACATATTCATGCAc > 1:773669/1‑143 (MQ=255) gTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCAc > 1:1094975/1‑143 (MQ=255) gTCCAGGAGAGTTGTTGACCGAGTTTCAGGTTGCGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCAc < 1:465011/143‑1 (MQ=255) gTCCAGGAGAGATGATGACCGAGGCTCAGGGGACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCAc < 1:711091/143‑1 (MQ=255) agagTTGTTGACCGCTTTTCAGCTTACACAATTCTTTGTCGGCCGCAGCCAGTTGCGTGAGAT‑CACACCCAGCAACACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCt < 2:74693/143‑1 (MQ=255) gttgACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCagag > 1:251299/1‑140 (MQ=255) ttgGCCGATATTCAGGTTACGGAATTCTTTGTCGGCCGCAGCCAGTTGGGTGACAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCgg < 2:514162/143‑1 (MQ=255) ccGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCCTACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACAAATTCATACCCGCCAGCTTCACCAGcaaa > 2:440787/1‑140 (MQ=255) cGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATCT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGAtt < 1:585263/143‑1 (MQ=255) cGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGAtt > 1:61536/1‑143 (MQ=255) ttCAGGTCACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTc < 2:424722/143‑1 (MQ=255) aGGTTACGCAATTCTTTGGCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGTCCGCTTCACCGGCGATTTTGTCATc < 2:31155/143‑1 (MQ=255) ggTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATcc > 1:777940/1‑143 (MQ=255) ggTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATcc > 1:351659/1‑143 (MQ=255) gCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATCCCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATCTTGTCCTCCAgcgcg > 2:489969/1‑139 (MQ=255) gCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCg > 1:1078614/1‑143 (MQ=255) aaTTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTc < 1:321355/143‑1 (MQ=255) aaTTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACACAAATCAATCCCATACTGATTGAGAATGCTGCTCAGCGTATTGCCCGTGGAAACAAAATAATCATGCACGCAAACATCACCGGGGAATCTGCCATCCAgaccctc > 2:226665/1‑137 (MQ=255) ttCCTTGTCGGCCGCTGCTAGTTGGCGTATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCt < 2:854410/143‑1 (MQ=255) tCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTg < 1:799179/143‑1 (MQ=255) gTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGaa < 1:694457/143‑1 (MQ=255) gatatGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGCTTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCAtctt > 1:751182/5‑143 (MQ=255) gTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCAtcttct < 1:385389/143‑1 (MQ=255) ggTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTtgtg < 1:1129698/143‑1 (MQ=255) ggTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGCCCTGGGGAATGGCTTCATCTTCTtgtg < 1:465529/143‑1 (MQ=255) tGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCa < 2:201603/143‑1 (MQ=255) tat‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCt > 2:1102306/1‑143 (MQ=255) t‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTg > 1:1053447/1‑143 (MQ=255) cACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGa < 2:61536/143‑1 (MQ=255) aCCCCTCTCAATACCATACTGATTGAGAATGCCGCTTAGCGTATTGCCAGTGGAAACAACATCTTCCTGCACGCCCGCCTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGCATGGCTTCATCTTCTTGTGCAGCTTGAt < 1:560824/143‑1 (MQ=255) aCCCATATCAATACCATACTGATTGAGAATGCTGCTGAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGAt < 2:1022323/143‑1 (MQ=255) aCCCATACCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGAt < 1:85338/143‑1 (MQ=255) atatCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAAt > 2:923111/1‑143 (MQ=255) atatCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGGGCAGCTTGATCAAt > 2:271936/1‑143 (MQ=255) aTACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGCGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGCTTTTGTCATCCAGTTCGTCCTGGGGCATGGCTTCATCTCCTTTTGCAGCTTGAACCATCGGCTc > 2:214146/1‑143 (MQ=255) aCCATGCTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACGAGCGCATGCACTCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCAc < 1:833310/143‑1 (MQ=255) ccATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCCCCGGCGAGTTTGTCAGGCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACt < 1:481578/143‑1 (MQ=255) agaCTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTgg < 2:233722/141‑1 (MQ=255) agtGAGAATTCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCAGGGGAATGGCTTCAGCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAg < 2:1094221/141‑1 (MQ=255) ttGAGAATGCTGCTTAGCGTATTGCCAGGGGAAACAACATATTCATGCACGCCTGCTTCACCGGCGATTTTGTCATCCATATCGTCCTGGCGAATGGCTGCATCTTCTTGTGCAGCTCGATCAATCGCCTCTTGGGCTTCTgg > 1:215366/1‑143 (MQ=255) gaATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATACACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGCAGAATGGCTTCATCTTCTTGTGCAGCTTGCTCACTCGGCTGACAGACTTCACGTGag > 2:610532/1‑143 (MQ=255) attgctgcTTATCGTTTTGCCTGTGGAACCAACATATTCCTGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCTTGGGGGATGGCTTCATCTTCGTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAaga < 2:895340/141‑1 (MQ=255) ctgctTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGcga > 2:341960/1‑143 (MQ=255) cGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGCGCGAACGAAttt > 1:426694/1‑143 (MQ=255) | CGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATAT‑CACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGCGAACGAATTT > NC_000913/1941194‑1941463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |