Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,872	A→G	V87A (GTA→GCA)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,872	0	A	G	100.0%	136.2 / NA	48	V87A (GTA→GCA)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

TGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGA  >  NC_000913/2661734‑2662009                                                                                                                                           |                                                                                                                                          tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGCCAGCGGGTGGCATCTGCAGa                                                                                                                                       >  1:710156/1‑143 (MQ=255)  ggTTATTAACCAGTTCGCCTAGAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGAt                                                                                                                                      <  2:834889/143‑1 (MQ=255)  ggTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGAt                                                                                                                                      >  1:1145488/1‑143 (MQ=255)  ggTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGAt                                                                                                                                      >  1:329965/1‑143 (MQ=255)    tttttaACCAGTTCGTCTAAAGTAATTTCGTTGAGAAAATCGGTTAGACGGTCGCTCAAATCACGCCACCGCGCGTGGGTCAGGCATTTATGGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTc                                                                                                                                    <  2:439131/140‑1 (MQ=255)    ttattaTCCAGTGCGCCTGAAGTAATGTTGTTGAGTAAGCCGGTGAGACGGTCGCTCAAATCACGCCGCAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTc                                                                                                                                    <  2:288452/143‑1 (MQ=255)    ttattaACTTGTTCGCCTATAGTTATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTc                                                                                                                                    <  1:410212/143‑1 (MQ=255)      attcACCAGTTCGCTTAACGTAATGTTGTTGAGACAACCGGTGCGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGt                                                                                                                                  <  1:78882/143‑1 (MQ=255)       ttaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTc                                                                                                                                 >  1:785413/1‑143 (MQ=255)              gTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCAGCCTCTCCCCTGACAACGGGTGGCAGCTGCGGCTTCGTCAACGgcg                                                                                                                          >  2:1113358/1‑143 (MQ=255)                   ccTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGGTAAt                                                                                                                     >  1:1059768/1‑143 (MQ=255)                     tAAGGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGACAGGCATTTATCGCAGGCCTGGCAGCCGCCTTTACCACGACGACGGGTGGCTACTGCAGTATCGTCAACGGCGATAGttg                                                                                                                   >  2:439692/1‑142 (MQ=255)                     tAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACCACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTa                                                                                                                   >  2:581893/1‑143 (MQ=255)                         gtcaTGTTGTTGAGATAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTc                                                                                                               <  2:1063166/140‑1 (MQ=255)                          tAATGTTGTTGAGAACACCGGTCAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCg                                                                                                              <  1:350525/143‑1 (MQ=255)                          tAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCg                                                                                                              >  1:62158/1‑143 (MQ=255)                                gggtgtgaAAACCGGTGGGACGGTCGCGGACGTGACGCCGCAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACGCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACg                                                                                                        <  2:1044576/137‑1 (MQ=255)                                  ttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCGGATTCGGCCACGGCGCGAAGGACTGCGCCAACGGc                                                                                                      >  1:327110/1‑143 (MQ=255)                                  ttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGc                                                                                                      >  2:99756/1‑143 (MQ=255)                                   tgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCCGACCACGGGTGGCATCTGCCGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCg                                                                                                     >  2:17561/1‑143 (MQ=255)                                     gcgagaCCGGTGAGACGGTCGCTCCAGTCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAt                                                                                                   <  2:779255/138‑1 (MQ=255)                                          aCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCCGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGCCCACGGCGCTAATTACTTCGCACACGGCgcggccgc                                                                                              >  1:709507/1‑136 (MQ=255)                                                           tCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc                                                                             <  1:413103/143‑1 (MQ=255)                                                           tCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc                                                                             <  1:995606/143‑1 (MQ=255)                                                           tCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACCGCGCTAATTACTTCGACAACGGCGATGCTGCTGGCATCTTTGCCTAAc                                                                             >  1:1134567/1‑143 (MQ=255)                                                            gcaaTCACGCCACCGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACa                                                                            <  2:728325/141‑1 (MQ=255)                                                            gaaaGCACGCCGCAGCGCGTTGGTCAGGCATTTATCGCCGCCGTGGCAGCGGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACa                                                                            <  2:34091/142‑1 (MQ=255)                                                                       acaGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCg                                                                 <  1:508496/143‑1 (MQ=255)                                                                                         aTTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTg                                               <  2:329965/143‑1 (MQ=255)                                                                                              tCGCCGCCCTGGCGGCCGCCGTTACCCTGACAACGGGTGGCAGCTGCAGATTCGTCAACCGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGGACGCTGGAAAc                                          <  2:384279/143‑1 (MQ=255)                                                                                              tCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAc                                          <  1:238503/143‑1 (MQ=255)                                                                                                gccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTCCTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTACCAGATAACCACCGCCTGGTCCACGCACGCTGCAAACCa                                        >  2:455616/1‑143 (MQ=255)                                                                                                 ccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAg                                       >  2:1026827/1‑143 (MQ=255)                                                                                                    ccccGGCAGCCGCCCTGACCCTGACAACGGGTGGCATCTGCAGACTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCCGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGAcc                                    <  1:728155/143‑1 (MQ=255)                                                                                                         gCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCAtttt                               >  1:737587/1‑143 (MQ=255)                                                                                                          cAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCAttttt                              <  1:432777/143‑1 (MQ=255)                                                                                                           aGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTa                             <  2:683158/143‑1 (MQ=255)                                                                                                             ccgccTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCCTTCTTAcc                           >  2:794223/1‑142 (MQ=255)                                                                                                             ccgccTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACg                           >  2:1033429/1‑143 (MQ=255)                                                                                                             ccgccTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCCGGTCCCCGTACGCTGGAAACCAGACCATTTCTACg                           >  2:131740/1‑143 (MQ=255)                                                                                                               gccTTCTCCCTGGTAACGGGTGGAATCTGCAGATTCGGCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCa                         <  1:439692/143‑1 (MQ=255)                                                                                                                  tttACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGAc                      >  1:560678/1‑143 (MQ=255)                                                                                                                   ttACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACg                     <  1:557179/143‑1 (MQ=255)                                                                                                                      cccTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTCTACGCAGACGGGa                  >  1:1109500/1‑143 (MQ=255)                                                                                                                              aCGTGTGGCATCTGCAGATTCGTTATCGGCGTTAATTACTTCGCCAACGGTGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAgtt          <  1:548756/143‑1 (MQ=255)                                                                                                                                gagTGCCATCTGCAGATTCATCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTCTCCCTAACACATAACCACCGCCTGGTGCACGTACGCTGGAAACCAGACCATTTTTCCGCACACGGGACAagagccgc        >  2:377402/3‑139 (MQ=255)                                                                                                                                    ggCATCTGCAGATTCGTCAACGCCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCa    <  1:694055/143‑1 (MQ=255)                                                                                                                                      cATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGa  >  1:987465/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                          TGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGA  >  NC_000913/2661734‑2662009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑