| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,486,157 | A→C | E13A (GAA→GCA) | crp → | DNA‑binding transcriptional dual regulator CRP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,486,157 | 0 | A | C | 97.9% | 138.9 / NA | 48 | E13A (GAA→GCA) | crp | DNA‑binding transcriptional dual regulator CRP |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (30/17); total (31/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAG > NC_000913/3486017‑3486294 | aGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCACACCGCAACCAGACCCGACTCGC‑GCAt > 2:1009041/1‑143 (MQ=255) gTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATg < 1:543583/143‑1 (MQ=255) cccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTCATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCACGGTTCTTGtc > 2:475668/1‑143 (MQ=255) ccccGGTAGCGGGAGGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCAtt < 2:295919/143‑1 (MQ=255) cccAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCAtt > 2:1089215/1‑143 (MQ=255) aGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTCATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACAt < 1:28468/143‑1 (MQ=255) cATATTTCGGCAAGCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTa < 1:132539/143‑1 (MQ=255) atatTTCGGCAATCCAGAGACAGCGGCGTTATATGGCTCTGGAGAAAGCTTATAGCAGAGGATAACCACGCATGTTGCTGGGCAAACTGCGGACAGACTCGACTCAC‑GCGGGGTTCTGGTCGCCGTGCCACAGTCATATGtcc > 2:193378/1‑141 (MQ=255) atTTCGGCAACCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTAccc < 1:710368/143‑1 (MQ=255) tttCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTCCCCa > 1:65141/1‑143 (MQ=255) tttCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCa > 1:973330/1‑143 (MQ=255) cGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCCCAAAGAGACCCGACTCTC‑GCATGATTCTTGTCTCAGTGCCACATTAAAAATTACCCATca > 2:799711/1‑142 (MQ=255) gCAATCCAGACACAGCGGCGTTATCTGGCCCTGGAGAAAGCTTATAACAGAGGATCACCGCGCATGGTGCTTACCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCaa < 1:488795/143‑1 (MQ=255) tCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGc < 1:170518/143‑1 (MQ=255) gagaCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGc < 2:451022/143‑1 (MQ=255) ccAGCGGGGTTATCTGGCTCTGGAGTAAGTGTATAACAGAGGATAACCGCTCATGGTGCTTGGCAAACCTCAAACAGACCCGACTCTC‑GCCTGGTTCTTGTCTCATTGCCACATTCATAAGTTCCCATCCAAGAGCACGCTTa < 2:1055794/142‑1 (MQ=255) cgcgtcgTTATCTGGCACTTGCGAAAGCCTGTACCACAGCATACCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGGGTCTC‑GCACGGTTCTTGTCTCATTGCCACTTTCATAAGTACCCATCCAAGAGCACGCTTAtt < 2:491775/142‑1 (MQ=255) cggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCa > 1:729648/1‑143 (MQ=255) cggcgTGATCTGGCTGTGGAGAAAGCTCATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCa < 2:789817/143‑1 (MQ=255) ggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCAGCACCAC‑TCCCTTTTCGTGTCTCCATGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCAc > 1:261256/1‑143 (MQ=255) ggcgTTATCTGGCTCTGGAGAAAGCATATAACAGAGGATAACCGCGCATGGTAAATTGCAAACCGCAAAATGACCGGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTAGCCATCCAAGAGCACGCTTATTCAc > 2:814189/1‑143 (MQ=255) gcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCAcc > 1:760447/1‑143 (MQ=255) cggtATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa < 2:460874/140‑1 (MQ=255) cgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCACCAGTGCCCATCCCAGAGCACGCTTCTTCAccc > 2:232755/1‑142 (MQ=255) tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAggg < 1:865112/143‑1 (MQ=255) tGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGCCTCTC‑GAATGGTTATTGTCACATTGCCACATATATAAATAACAACACAAGAGCACTATTATTCACCAGAGTGaa > 2:768350/1‑143 (MQ=255) gCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCGAAGAGCACGCTTATTGACAAGGGTGaaaa > 1:938012/1‑143 (MQ=255) tctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAg > 1:447709/1‑143 (MQ=255) ctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGc > 1:841559/1‑143 (MQ=255) tGGAGCAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCCAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACGTTCATCACTACCCATCCAAGCGCACGCTTCTCCACCAGGGTGACACAGCg > 2:197663/1‑143 (MQ=255) gggaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGCCTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa < 2:726070/141‑1 (MQ=255) aGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACAGAAAACAGAGACGACTCTC‑GCATGGTTCTTGTGTCATTGCCAAATTCATAAGTACACATACAAGAGCACGCTTATTCACCAGGGTGAAAAAGAGGAAACGc > 2:162636/1‑143 (MQ=255) ttATAACAGAGGATAACCGCGCATGGTGCTTGGCTAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAACGCGGAAACGCCGt > 2:759554/1‑143 (MQ=255) ttATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGCGCACGCTTATTCACCCGGGCGACACAGCCGCAACGCTGt > 2:542573/1‑143 (MQ=255) ataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtac < 2:841559/143‑1 (MQ=255) gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCCTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGACACGCTGTACTACATcacg > 1:511365/1‑140 (MQ=255) aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGATCTTGTCTCATTGCAACATTTATAAGTACCCCACCAAGAGCACCCTTATTCACCAGCGTGAAAAACCGGAAACGCTTTCCTAAATCgcgcaa > 2:660304/1‑138 (MQ=255) cgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc > 1:823198/1‑143 (MQ=255) cTTGGCAAACCGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGt > 1:533798/1‑143 (MQ=255) cAAACCGCAAACAGACCCGAC‑CTCAGCTTGGTTCTTGTCTCATTGCCACATTCATGAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGTAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGa < 1:207248/143‑1 (MQ=255) cGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCCTTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTGCTACATCGTTAAGGGCTCAGCAGCCGAGGTGATCaaa > 2:571724/1‑143 (MQ=255) cGCAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaaa < 2:1028493/143‑1 (MQ=255) cAAACAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGa > 2:1147346/1‑143 (MQ=255) aCAGACCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa > 2:300192/1‑143 (MQ=255) aCCCGACTCTC‑GCGTGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCCCGCTTATTCACCAGGGTGACAAAGCGGAAACGCTGTACCACCTCGTTAAAGGCTCTGGGGCAGCGCTGATCAAAGACGAagag > 2:302079/1‑143 (MQ=255) aCCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCACGAGCACGCTTGTTCACCAGCGTGACAAACCGGAAAAGCTACACTACGTCGTTAACGACACTTAGGGAGCGCTGATCAAAGCCAAagcg > 2:287778/1‑141 (MQ=255) aCCCGACTCTC‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACACATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAACGCTCTGTGGCAGTGCTGATCAAACACGAagag > 1:1031897/1‑143 (MQ=255) tctc‑GCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTCCTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAg > 1:935997/1‑143 (MQ=255) | AGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTC‑GAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAG > NC_000913/3486017‑3486294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |