Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	710,489	G→C	A53G (GCT→GGT)	fur ←	DNA‑binding transcriptional dual regulator Fur

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	710,489	0	G	C	100.0%	186.3 / NA	58	A53G (GCT→GGT)	fur	DNA‑binding transcriptional dual regulator Fur
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (30/28);  total (30/28)

TAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGC  >  NC_000913/710349‑710628                                                                                                                                             |                                                                                                                                            taccccagCCGCGGTGGAGGCAGTTGTCGTTATCGTGGAGCTGGCGCTGTGTCAGTTCACATACGCATTTACCGCCTTCAAAATCGTGGCCGGTGACGATACCACCGCCGTCAAACTGGTTCAGTACGCGATATACCGTAccc                                                                                                                                           <  2:566097/136‑1 (MQ=255) tAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAccc                                                                                                                                           >  1:975676/1‑143 (MQ=255)    gcTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGGTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGa                                                                                                                                        <  2:177458/142‑1 (MQ=255)    ccTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACCGGTTCAGTACGCGATATAACGTACCCaac                                                                                                                                        >  2:166840/1‑141 (MQ=255)     cTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAACCTGGTTCAGTACGCGATATACCGTACCCAGAc                                                                                                                                       >  1:94008/1‑143 (MQ=255)     cTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGCTCAGTACGCGATATACCGTACCCAGAc                                                                                                                                       >  1:390484/1‑143 (MQ=255)         ccGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGCGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAAt                                                                                                                                   <  1:381035/143‑1 (MQ=255)          cGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCACACTGGTGCAGTACGCGATATACCCTACCCAGACCaagt                                                                                                                                  >  2:767484/1‑141 (MQ=255)            cAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATttc                                                                                                                                <  1:345650/143‑1 (MQ=255)                           tggTGATCGGCGTGAGGTTGCTGTGTCACGTCGCATACGGCTGTACCGCCTTCAAAATTGCGGCTGGTGAGGAGACCGGCGTCGTCCAACTGGTTCAGTACGCGAGATACCGTACCCAGACCAATTTCTTCACCCATATCGAt                                                                                                                 <  2:432600/142‑1 (MQ=255)                           aGGTGACCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGAGTTACCGCCTTCAAAATTGTGGCGGGTGACGATAGCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt                                                                                                                 <  2:573377/143‑1 (MQ=255)                           aGGTCATCGTGGTGATGCTGCCGTGTCGGTTCGATGACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt                                                                                                                 <  2:772376/143‑1 (MQ=255)                             gTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCa                                                                                                               >  1:791979/1‑143 (MQ=255)                                  cGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGt                                                                                                          <  2:246571/143‑1 (MQ=255)                                    tggtgATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAAGTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGttt                                                                                                        <  2:785355/143‑1 (MQ=255)                                        gATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCATTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGCTCAGACGGTTGtat                                                                                                    >  1:1090634/1‑143 (MQ=255)                                               cTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCACACTGGTTCAGTACGCGATATACCGTACCCAGACCCATTTCTTCACCCATATCGATACGACGTTTGtatcgctctc                                                                                             >  2:932399/1‑136 (MQ=255)                                                tgtgTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCCATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTc                                                                                            >  1:1119067/1‑143 (MQ=255)                                                 gtgtCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTcc                                                                                           <  2:667354/143‑1 (MQ=255)                                                  tgtCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCg                                                                                          <  1:734577/143‑1 (MQ=255)                                                          cAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACg                                                                                  >  1:701587/1‑143 (MQ=255)                                                            aaTACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCCTATCGATCAGACGTTGGTATAAATCTTCCGCACTGaaggg                                                                                >  2:671620/1‑139 (MQ=255)                                                            aaTACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTAAGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAACTCTTACGCACTGACGTg                                                                                >  2:421051/1‑143 (MQ=255)                                                               aCGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGGACGCGAGATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGGATAAATATTCCGCACAGACGTGAtc                                                                             >  2:537103/1‑142 (MQ=255)                                                               aCGGATTTACCCCCTTCAAAAGTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATg                                                                             <  2:416767/143‑1 (MQ=255)                                                                       aCCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCg                                                                     <  2:770856/143‑1 (MQ=255)                                                                        ccGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCgg                                                                    <  2:649563/143‑1 (MQ=255)                                                                         cGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAAATGGTTCAGGACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTTTAACTCTTCACCACTTCGGTGATGGTTGTCCgca                                                                   >  2:519108/1‑141 (MQ=255)                                                                             ttCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATAAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCCGCTCCt                                                               >  2:1056768/1‑143 (MQ=255)                                                                               cAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTga                                                             >  2:941435/1‑143 (MQ=255)                                                                                       tgGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTc                                                     <  1:772014/143‑1 (MQ=255)                                                                                         gCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTccg                                                   >  1:309085/1‑142 (MQ=255)                                                                                         gCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCa                                                   <  1:1023686/143‑1 (MQ=255)                                                                                         gCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCa                                                   >  1:399244/1‑143 (MQ=255)                                                                                         gCGGGTCACGATACCAGCGTCGGCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACAGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCa                                                   <  2:91139/143‑1 (MQ=255)                                                                                          cGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAg                                                  <  1:552064/143‑1 (MQ=255)                                                                                           gggTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAACTCTTCCGCACTGACGTGATGGTTGTCCTGCTCCTGAAGAACTTCCAgg                                                 >  1:701802/1‑143 (MQ=255)                                                                                              tgccGATACCCGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGAtt                                              <  2:256918/140‑1 (MQ=255)                                                                                               gACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGAttt                                             <  1:1074764/143‑1 (MQ=255)                                                                                                 cGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGGACCCAGACCAATTTCTTCACCCACATCGATCCGACGTTTGTACAAATCTTCGGCACTCACGCGATGGTTGTCCGCCTCCTGAACAACGTCCCGGActttt                                           >  2:218708/1‑143 (MQ=255)                                                                                                    tACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTaaa                                        <  1:247018/143‑1 (MQ=255)                                                                                                     aCCAGCGTCGTCAAACTGGTCCAGGACGCCATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTGTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAAc                                       <  1:391749/143‑1 (MQ=255)                                                                                                       cAGCGTGGTCAAACTTGTTCAGTACGCGATATACCGTACCCAGTCCAAGGTCTTCACCCATATCGAGCAGACGTGTGTATAAACCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGa                                     <  1:13081/143‑1 (MQ=255)                                                                                                            tcgtcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGAATTTTAAACGAGAAAg                                >  2:259677/1‑143 (MQ=255)                                                                                                              gtcAAACTAGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCCGAAGAACTTCCAGGATTTTTAAACGAGGAAGCg                              <  1:417324/143‑1 (MQ=255)                                                                                                               tcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCCCTGACGTGATGGTTGTCCGGCTCATGAAGAACTTCCAGGAGTTTTAAGCGCAGAAGCGt                             >  2:858897/1‑143 (MQ=255)                                                                                                               tcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGCCGTGACGGCTGTCGGGCTCCTGACGAATAACTAGATTGGTTAAACGAGGAAGCGt                             >  1:1021545/1‑143 (MQ=255)                                                                                                               tcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGt                             >  1:693817/1‑143 (MQ=255)                                                                                                                  aaCTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTAc                          >  1:681228/1‑143 (MQ=255)                                                                                                                     tgtttCACTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCTCTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACttt                       <  2:1119087/140‑1 (MQ=255)                                                                                                                        ttCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTAAg                    >  2:618772/1‑143 (MQ=255)                                                                                                                        ttCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTATTAAACGAGGAAGCGTTACTTTCAg                    >  2:407954/1‑143 (MQ=255)                                                                                                                          cAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGc                  <  1:123876/143‑1 (MQ=255)                                                                                                                            gTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCa                <  1:616076/143‑1 (MQ=255)                                                                                                                             tACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGACGCGTTACTTTCAGGCCAg               >  2:1107211/1‑143 (MQ=255)                                                                                                                              aCGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGc              >  2:165738/1‑143 (MQ=255)                                                                                                                                  gATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAAGTTCCAGGATTTTTGAAGGAGGAAGGGGTGCTTGCAGGGCAGcgggc          >  1:123919/1‑139 (MQ=255)                                                                                                                                  gATATACCGTACCCAGACCAAGTTCTTCACCCAGATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGctttc          <  2:325569/143‑1 (MQ=255)                                                                                                                                       aCCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAg     >  1:125556/1‑143 (MQ=255)                                                                                                                                          gTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGc  <  1:516790/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                            TAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGC  >  NC_000913/710349‑710628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑