Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,026,927	A→T	V30E (GTG→GAG)	mgsA ←	methylglyoxal synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,026,927	0	A	T	100.0%	134.3 / NA	44	V30E (GTG→GAG)	mgsA	methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/23);  total (21/23)

TGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATC  >  NC_000913/1026788‑1027064                                                                                                                                            |                                                                                                                                          tGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAACTTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGATGTTCCAGTAACGGTTGGTGCCGCTCCTccc                                                                                                                                        >  2:1135544/1‑143 (MQ=255) tGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTccc                                                                                                                                        <  2:304939/143‑1 (MQ=255)    gcCAACCTGCTGGTCACCCCCCATTAGGCCACTCAACATCGCGTTGCCGTTCATGCCGGTCGCGCGGGCAATTCAGTTACCGGTAGTGCCTGCTGCATACAGTACGTGTTCTTCCAGTAACGGTGTATGCCGTTCCTCCCAGc                                                                                                                                     >  2:73651/1‑143 (MQ=255)      cAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTGGTGGCCGTTCCTCCAAGCtc                                                                                                                                   >  1:33826/1‑143 (MQ=255)        aactgctgGGCAGCCCCCATTGGGCCACTCAACACCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCtcat                                                                                                                                 <  1:885348/141‑1 (MQ=255)            gctgGTCGCCCCCCTTTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGc                                                                                                                             <  2:568393/143‑1 (MQ=255)            gctgGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGc                                                                                                                             >  2:307287/1‑143 (MQ=255)            gctgGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGc                                                                                                                             >  1:756459/1‑143 (MQ=255)                   cccccccATTGGGCCCCTCTACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCAtttgtt                                                                                                                      <  2:354679/142‑1 (MQ=255)                       cccGTTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCa                                                                                                                  <  2:8051/143‑1 (MQ=255)                          aTTGGGCGACTCAACATCGCGTTGGCGTGCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTg                                                                                                               <  2:700551/143‑1 (MQ=255)                          aTTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATGAGCATTTGTTTGTAGTg                                                                                                               >  2:397483/1‑143 (MQ=255)                          aTTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTg                                                                                                               <  1:633413/143‑1 (MQ=255)                                 cACTCATCATCGCTTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCgtgt                                                                                                        <  2:191936/143‑1 (MQ=255)                                 cACTCAACAGCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCgtgt                                                                                                        <  1:752044/143‑1 (MQ=255)                                       aCATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGGAGGGATCGTGTTccacg                                                                                                  >  1:369977/1‑142 (MQ=255)                                        cATCGCGTTGACGTTCATGCCGGTCGCTTGTTACTTTTTGTTTTCTGTAGTGCCTGTTGCATACAGTACGTGTTGTGCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGccacca                                                                                                 <  1:306819/143‑1 (MQ=255)                                           cgcgTTGACGGTGATGCCGGTCGCGTGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAgcg                                                                                              <  2:612205/143‑1 (MQ=255)                                               ttGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAat                                                                                          >  1:14729/1‑143 (MQ=255)                                                   cGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGt                                                                                      <  2:787047/143‑1 (MQ=255)                                                     ttCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCCACCCAATATGtgt                                                                                    >  2:602716/1‑141 (MQ=255)                                                     ttCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGttt                                                                                    <  2:468414/143‑1 (MQ=255)                                                          gCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGGGCCACCACCGCCATACGTTTCcccg                                                                               >  2:1063739/1‑143 (MQ=255)                                                             ggTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAg                                                                            <  1:328626/143‑1 (MQ=255)                                                               tCGCGCGGGCAATTAAGTTACGGGTAGGGCCTTTTGCATACAGTACGTGTTGTTCCGGTAACGGTTGAGGCTGTTCCTCCCAGCTCATTAGCATTTGTTTTCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGt                                                                          <  2:585677/143‑1 (MQ=255)                                                                  cgcgcgAAATCAACTGACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTaaa                                                                       <  1:709127/143‑1 (MQ=255)                                                                  cgcgGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCGCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTaaa                                                                       <  2:279963/143‑1 (MQ=255)                                                                    cgGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGt                                                                     <  1:1089167/143‑1 (MQ=255)                                                                           ttAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCCATCTGTTTCCGCGCAGGTAAAGTGCGAgtc                                                              >  1:1117247/1‑143 (MQ=255)                                                                                       tAGTGCCTGTTGCATACAGTACGTGTGGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGAAACGTGCGAGTCGTCAGTTCCATa                                                  >  2:882305/1‑143 (MQ=255)                                                                                                     tACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAg                                    >  1:893161/1‑143 (MQ=255)                                                                                                      aCAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGCTCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGt                                   >  1:1046400/1‑143 (MQ=255)                                                                                                            cGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCACAGGTAAAGTGCGAGTCGTCGGTTCCATAATGTACATCCGAAGTTAAcat                             >  2:53578/1‑141 (MQ=255)                                                                                                              tgttgtTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTGTGCAGTGATCGTGCGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTc                           <  1:715835/143‑1 (MQ=255)                                                                                                                  gttCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCACTATGTCTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTAc                       >  2:382920/1‑143 (MQ=255)                                                                                                                       aGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCACTATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTCCCATAAAGTACATCCGTAGTTAACTTTCCTACAGagt                  >  2:2142/1‑141 (MQ=255)                                                                                                                       aGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGAtt                  >  1:56485/1‑143 (MQ=255)                                                                                                                         caaCGGTTGATGCCGTTCCTCTCAGCTCATCAGCATCTGTTTGCCGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAAGTAACGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTAc                <  1:141669/142‑1 (MQ=255)                                                                                                                         caaCGGTGGATGCTGTCCCTGCCATCTCATCAGCATTCGTTTGCAGTGATCTTTTGCCACCAGCGTAATTTTTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTAc                <  1:780542/142‑1 (MQ=255)                                                                                                                            cGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGt             <  2:14729/143‑1 (MQ=255)                                                                                                                             ggTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCCGTTCCGTAATGTACATCCGTAGTGAACTTTCCTACAGACTACTGTa            >  1:1020622/1‑143 (MQ=255)                                                                                                                                  aTGCCGTTCGTCCTAGCTCATCAGTATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCAc       <  1:333278/143‑1 (MQ=255)                                                                                                                                       gTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCGGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATc  >  1:372429/1‑143 (MQ=255)                                                                                                                                       gTTCCTCCCAGCTCATCAGCATATGTTTGCAGTGATAGTGTGCCACCAGCGCACTATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAAATTTCCTACAGATAACCCCAAGCACTTATc  >  2:651966/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                          TGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATC  >  NC_000913/1026788‑1027064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑