mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Glucose_ALE_4_FLASK_237_Isolate_1_S3_L001_R1_001.good.fq | 1,620,875 | 271,410,797 | 100.0% | 167.4 bases | 239 bases | 99.5% |
| errors | Glucose_ALE_4_FLASK_237_Isolate_1_S3_L001_R2_001.good.fq | 1,620,875 | 271,381,836 | 100.0% | 167.4 bases | 239 bases | 96.5% |
| total | 3,241,750 | 542,792,633 | 100.0% | 167.4 bases | 239 bases | 98.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 118.5 | 6.0 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 14304 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 31 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.003 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.78852 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 07:43:19 13 Jun 2021 | 07:44:07 13 Jun 2021 | 48 seconds |
| Read alignment to reference genome | 07:44:08 13 Jun 2021 | 07:51:49 13 Jun 2021 | 7 minutes 41 seconds |
| Preprocessing alignments for candidate junction identification | 07:51:49 13 Jun 2021 | 07:52:34 13 Jun 2021 | 45 seconds |
| Preliminary analysis of coverage distribution | 07:52:34 13 Jun 2021 | 07:54:47 13 Jun 2021 | 2 minutes 13 seconds |
| Identifying junction candidates | 07:54:47 13 Jun 2021 | 07:55:14 13 Jun 2021 | 27 seconds |
| Re-alignment to junction candidates | 07:55:14 13 Jun 2021 | 07:57:01 13 Jun 2021 | 1 minute 47 seconds |
| Resolving best read alignments | 07:57:01 13 Jun 2021 | 07:58:02 13 Jun 2021 | 1 minute 1 second |
| Creating BAM files | 07:58:02 13 Jun 2021 | 07:59:58 13 Jun 2021 | 1 minute 56 seconds |
| Tabulating error counts | 07:59:58 13 Jun 2021 | 08:00:46 13 Jun 2021 | 48 seconds |
| Re-calibrating base error rates | 08:00:46 13 Jun 2021 | 08:00:47 13 Jun 2021 | 1 second |
| Examining read alignment evidence | 08:00:47 13 Jun 2021 | 08:10:32 13 Jun 2021 | 9 minutes 45 seconds |
| Polymorphism statistics | 08:10:32 13 Jun 2021 | 08:10:32 13 Jun 2021 | 0 seconds |
| Output | 08:10:32 13 Jun 2021 | 08:10:46 13 Jun 2021 | 14 seconds |
| Total | 27 minutes 26 seconds | ||
