Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	3,815,859	Δ82 bp	100%		[rph]	[rph]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3815859	3815940	82	48 [0]	[1] 50	[rph]	[rph]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3815858	0 (0.000)	43 (1.660)	38/244	NT	100%	intergenic (‑90/+5)	pyrE/rph	orotate phosphoribosyltransferase/truncated RNase PH
	?	NC_000913	3815941 =	0 (0.000)					coding (609/687 nt)	rph	truncated RNase PH

CCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3815707‑3815858 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCT  >  NC_000913/3815941‑3816082                                                                                                                                                                                                                                                                                                         CCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGGTATGGTTTCATTCCTTCGCTCATCATCTTACTATTCTACAAACAAAAAAAAAGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCC                                                                                                                                                         >  1:167378/1‑143        TTAACACGTGCTTGTTAAGCGCAAATTCAATAAACTGGCGTTGATATGTTTTCATGCCTTCTCTCCTCATCTTACGTATCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCT                                                                                                                                                  <  1:45744/143‑1        TTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCGTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCT                                                                                                                                                  <  2:518475/143‑1        TTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCATCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTCAAAATCAGTTTGCCAGAGCCGCATTCT                                                                                                                                                  >  2:213049/1‑143           ACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCC                                                                                                                                               <  1:193696/143‑1                TGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCC                                                                                                                                          <  2:152091/143‑1                   TTGTTAAGCGAAAATTCAATAATCTGGCGCTGATATGGTTTCATGCCTTCGCTCTTCATCTTACTTTTCAACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTG                                                                                                                                       <  1:502416/143‑1                      CTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTATCTACAAACAAAAAAAAAAAGACAAATCA‑ATCACCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCAC                                                                                                                                    >  1:508910/1‑143                        AAGCGCAAATTCAATAAACTGGCGCTGAAATAGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑ATCACCTTAAAAATCAGTTTGCAAGGGCCGCCATCTGCCGTACACTGGACTT                                                                                                                                  >  2:6019/1‑143                                   CAATAAACTGGCGCTGATATGGTTTCATGTCTTCGCTCTTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACGCATCA‑GTCGACTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGC                                                                                                                       <  1:186629/143‑1                                    AATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCg                                                                                                                      >  1:260845/1‑142                                      TAAACTGGCGCTGATATGTTTTCATGTCTTCGCTCCTCATCTTACAGTTCTACAGACAAAAAAAAGGCGACGCAGCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCG                                                                                                                    <  1:162289/143‑1                                            GGCGCTGAGATGGCTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCG                                                                                                              <  2:472895/143‑1                                              CGCTGATATGGTTTCATGCCTTCGCTTCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGT                                                                                                            <  1:9530/143‑1                                              CGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTTCATACAATATATAGGCGTCTCATCA‑GTCGCCTTAAAAATCAGGTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGT                                                                                                            <  1:19590/143‑1                                                      TGGTTTCATGCCTTCGCTCCTATAGTTGCGTGTCGACAGACATAAAAAATGTGTCTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTA                                                                                                    <  1:513875/143‑1                                                         TTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCAACA‑GTCGGCTTAAAAATCAGTTTGACAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCACGACTTCGGTCACCACTcctg                                                                                                 >  2:508540/1‑139                                                           TCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAACAAGGCGACTCATCA‑GTCGCCTTAAAAATCCGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTACACTTCAATGATGCGCCCGTCCTCGGACCTCACCACGccc                                                                                               >  2:173696/1‑140                                                               gcgTTCGCTGCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCAGCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGT                                                                                           <  2:143850/140‑1                                                                 CTTCGCTCCTCATCTTACTTTTATACAGACAAAATAAAGGCGTTTCATCA‑GTCGCCTTAAAAATCAGTTGGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAAGGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCG                                                                                         <  1:192095/143‑1                                                                  TTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTCACAAAAAAATAACCAACGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATACACCCGTCTTCGATCATCACTACGTTCATGTCGG                                                                                        >  1:75576/1‑143                                                                       TCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCACTGATGCGCCCGTCTTCGCTCATCCCCACGCTCATGGCGCTCcca                                                                                   >  2:327426/1‑140                                                                         CTCATCTTACTTTTCTATTTACAAAAAAAAGGCTACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGC                                                                                 <  1:364390/143‑1                                                                          TCATCTTACTTTTCTACAGACAAAAAAAAGGCTACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCG                                                                                <  1:394072/143‑1                                                                                  ccTTTCTACGGACGAAAAAAAGGCGACTCACGAGGTCGAC‑CAGAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCTCTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC                                                                        <  2:112284/141‑1                                                                                   TTTTCTACAGACAAAAAAAAGGCGACTCATCG‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTTCCCTGCACTTCAATCATGCGCCCGACTTCGGTCATCACTACGTCCATGACGGACTCTGCGGCAGAGACT                                                                       >  1:441736/1‑143                                                                                   TTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATTATGCGCCCGTCTTGGGCCACCACCACGTTCATTTCGGTCCCTGCGGCAGAGTCT                                                                       >  2:243639/1‑143                                                                                     TTCTACATACTAAAGAAAGGCGATTCATCA‑GTCGCCTTAAAAATCAGTTCGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTC                                                                     <  1:378101/143‑1                                                                                      TCTATACACAAAAAAAAGGCGACTCACCA‑GCGGCCTGAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCA                                                                    <  1:72949/143‑1                                                                                      TCTACACACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCA                                                                    <  2:245179/143‑1                                                                                        TACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATTATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAAC                                                                  >  1:186966/1‑143                                                                                         ACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACG                                                                 >  1:487535/1‑143                                                                                          CAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGATTACAAGCGCCGCCTTCTGCGGTTCCCTGCCCTTAAATGACGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCCGCGGCAGAGTATTCAAAGT                                                                >  2:8178/1‑143                                                                                             ACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGCTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCACGTCTTCGGTCATCACTACGTTCATGTCGGTCACTGCGGCAGAGTCCTCCACGTATg                                                             >  2:446695/1‑142                                                                                              CAAAAAAAAGGCGCCTCATCA‑GTCGCCTTAAAAATCATGTTGCCGGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTC                                                            <  2:389396/143‑1                                                                                                  AAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCCTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGACATCACTACGTTAATGTCGGTCTCTACGGCAGAGTCTTCAACGTATTCCAGA                                                        >  1:263649/1‑143                                                                                                   agAAGGCGACTCATCA‑GTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTTCGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT                                                       <  2:40945/141‑1                                                                                                   AAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCAGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT                                                       <  2:360130/143‑1                                                                                                        GCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTATACAGAAATCACTACGTTCATGTCGGTCTATGCGGCAGAGTCTTCAACGTATTCCAGATCGCAA                                                  >  1:333474/1‑143                                                                                                              CATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCCATGATGCGCCCGTCTTCGGTCATCACTACATTCATGTCAGTATATACAGAAAAGTCTTCAACATATTCCAGATCACAAACCGCT                                            >  1:454709/1‑143                                                                                                                       GCCTTGAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGATCATGTCGATCTCTGCGACAGAGTCTCAAACGAATTCCAAATCGGAAACACGTTCGCCGTT                                    >  2:477811/1‑143                                                                                                                       GCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTTCACTTTTATGATGCGTCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCGGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTT                                    <  1:195290/143‑1                                                                                                                        CCTTAAAAATCAAGTCACCAGTGCCGTCTTCTGCCGTCCCCTGCACTTCACTGATGCGCCCGTCTTCGCTCATCACAACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTC                                   <  2:158415/143‑1                                                                                                                         CTTAAACATCAGTTTGCCCGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCA                                  <  2:440865/143‑1                                                                                                                               AATCAGTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACATTAATGTCGTTCTCTGCGGCAGAGTCTCCAACGTATTCCAGCACGCAAACAGCTTCGCCGTCCCCAATac                            >  2:339008/1‑141                                                                                                                                    GTTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGACTTCAACGTATTCCAGATAGAAAACCACTTCGCCGTTCACAATTCCGACA                       >  1:262919/1‑143                                                                                                                                     TTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCACTGCTGCGCCCGTCTTCGGTCATCACTAAAAACATGTAAATCTCTGCAGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAG                      >  1:36660/1‑143                                                                                                                                     TTTGCCAGCGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGTGTCTTCAACGTATTCCAAATTACAAACCCCTTCGCCGTTCACAATTCTGACAG                      >  1:82213/1‑143                                                                                                                                             CGCCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGCTCTCTGCGGCAGAGTCTTCAACGTATTCAAGATCGCAAACCGCTTCGCCGTTAACAATTCCGACAGAAACTGag              >  1:34818/1‑141                                                                                                                                               CCGCCTTCTGCCGCCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGCTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGCAACTGCGtc            >  1:405858/1‑141                                                                                                                                                 GCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAAAGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCTGACAGAAACTGCGGCTA          >  1:38825/1‑143                                                                                                                                                         CCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCTTTCACAATTCCGACAGAAACTGCGGCTACCATCCCT  <  1:367437/143‑1                                                                                                                                                                                                                                                                                                         CCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCA‑GTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3815707‑3815858 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCT  >  NC_000913/3815941‑3816082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.