| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0% | 40.4 / ‑6.7 | 22 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base C (12/5); minor base T (0/2); total (14/8) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132653‑2132926 | ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGGATTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAAAATCTTTTTGTTGACGAGGTGATCTGCATTTCACCGTGATTGATAAGAAGGAtgtgggac > 2:498954/1‑139 (MQ=255) cgctGTCTAATTTGTTTTTTTTCTTGCGTTAAAAGTGGAAGTACGCGGAGAATTTTGTCGGAGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc < 1:235917/143‑1 (MQ=255) ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTAGCCGGAGAATTTGTTGGGAGGCCACTTCATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCTGTACTccacc < 1:416981/143‑1 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCACGGTTCGCCGTACTccaacac > 2:327362/1‑143 (MQ=255) aaTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCTGAGTCTAGGTGAATTTGACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccccccccct > 1:232814/1‑141 (MQ=255) tttGTGCTTACGGTAAAAGTGAGAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTATGCGTAGCCACTTCTACTTTGCAGGGTTCGCCGTACTCAACCACCATCCGCAAGa > 2:197999/1‑143 (MQ=255) ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGGAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCCCCATCGGCAGGAAGATgcc > 2:140420/1‑141 (MQ=255) gtgacgaGTGGGAGTCCGCGGAGAATTCTTGCGGTGACGAGGTGATCTGCATTTCAGCGTGATTGATATGCAGGGTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTaa < 2:237445/137‑1 (MQ=255) gTGAAAGTACCCGGAGAATCTTTTCGGTGAAGAGGTGATCGACCTTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTCTTCAACTCTCCACGGTTCGCCGTACTCCTCACCCCACCGCAGCACGATGGCGAAAtattgtc > 2:368836/1‑137 (MQ=255) tactcatagaATTTTTTCGGTGACGAGGTGATTTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGACTCTTCTACTTTACACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt < 1:322011/136‑1 (MQ=255) ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGGTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGCGAGATGTCGTAATGCTGTGACGGTTTCAg > 2:374408/1‑143 (MQ=255) agaATTTTTTCGTTGACGAGGTGATCTGCATTTCATCGTGATTGATATGCAGGTTCTGTGTCGCCTCTTCTACTTGCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:86234/143‑1 (MQ=255) gATCTGCCTTTCTCCGTGATTGATATGCAGGCTCTGCTTCGCTTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa < 2:579383/143‑1 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCAGCAGGAAGATGACGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTGTGaa > 1:132161/1‑143 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTATCAGTTCGGTATCGAACAAACACTCTTTGAAAc > 1:26436/1‑143 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAATACACCACCATCCGCAGGAAGATGTCGTAATACTGTGCCGCTCTCAGGTCAGGATCGAATAGACACTCCATGAAAc > 2:576886/1‑143 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTCCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTGCAGTTCGGACACGAACCAACACTCATTGCAAca > 2:407680/1‑142 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCATCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCATGAAGATGTCGTAATCCGGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg > 2:320029/1‑143 (MQ=255) gCATTTCACCGTGATTGATATGCAGGTTCTGTTTCGCCTCTTCTACTTTGCAGGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc < 1:561314/143‑1 (MQ=255) gCATTTCACCGCGATTGATAGGCAGGCTCTGCGGCGCCTCTTCTACTTTCCACGGTTCGCGGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc < 1:7033/143‑1 (MQ=255) ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGCACTCCACCACCATCCGCATGAACATGTCGTAATCCTGTGCGGCGTTCAGCTCGGTAGCGAACGAACACCCTTTGAAACGCCCTTcccc > 2:105890/1‑142 (MQ=255) aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCCGCTCGGTCTCGCACATACACTCTCTGAACCCCCATTCCCCGGTACAGACTCGGtt > 1:89940/1‑143 (MQ=255) | GGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132653‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |