| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,489,152 | C→T | 59.7% | S6N (AGC→AAC) | yfdE ← | acetyl‑CoA:oxalate CoA‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,489,152 | 0 | C | T | 59.7% | 14.5 / 24.2 | 37 | S6N (AGC→AAC) | yfdE | acetyl‑CoA:oxalate CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base C (9/5); new base T (8/13); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGA > NC_000913/2489018‑2489278 | gggTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCCAAAGGTCCATTAAAGACATTTGTCATATCGA‑AAATAATAAACATTCAAACGGCCCTTTGCTTTCattat > 1:446193/1‑143 (MQ=255) tcaCCATGACCCGGCGACTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGATCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAAATAATAAGCCTTCAAACGACCCTTTGATTTCATTATTTGTCAt > 2:458438/1‑143 (MQ=255) gACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCACACCGCCCTTTGCTTTCATTATTTTTCATTTGATTa > 1:343993/1‑143 (MQ=255) ccGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTAtttcttattttcttatac > 1:386115/1‑141 (MQ=255) ccGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTTTCATTTGTTTATGc > 1:128528/1‑143 (MQ=255) cAACTTTAATTACCCTTGCTCCGATATTACAAAGAAGTTGAGTTCCGAAATGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGaaaaa < 2:283708/143‑1 (MQ=255) tcTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATATCTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAg < 2:252515/142‑1 (MQ=255) gcTTTATTTACGCTTGCGCCTATATTACAACGTTTTTGGTTTCTGTAATGTCCATTAAGGACATGTGTCATTTCGATAACTTATAAGCTTTCAAACTGCCCTTTTTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAg < 2:560778/142‑1 (MQ=255) tttAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTACGGACCTGTGTCATATCGATCACTACTAAGCCTTCAAACGGCACTTTATTTTAATAATTTGTCATTAGATTATGCCTTGAAAAAAGcc > 1:458609/1‑143 (MQ=255) ccTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTAATTATTTGTCATTTGCTTATGACTTGAAAAAAGCCGCAGGTGaa > 2:281221/1‑143 (MQ=255) ttGCGCTCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAAGAAGCCTTCAAACGGCGCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGaaaa < 2:166721/143‑1 (MQ=255) catgctatatTCCAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCGTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGa < 1:185259/137‑1 (MQ=255) atatTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTc < 1:246527/143‑1 (MQ=255) atTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACTGCCCTTTGCTTTCATTATTTGTCATTTGCTTATACCTTGAAAAAAGCCGAAGATGAAAAAACACTTCga > 1:173015/1‑142 (MQ=255) aaaGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAACAAGCCGAAGGTGAAAAGACACTTCTGCAtat > 1:238779/1‑141 (MQ=255) aaaGAAGTTAAGTTCCGAAAAGTGCATTAAGGGTAAGAGGTATATTTATAACAAATAAGCCTTCAAACGGCCCTTTATATTCAATATTTCTCATTTGCTCATAGCGTGATAAAAAGCGAAGGTGAAAAACAACTCCCGCAttt > 2:341890/1‑143 (MQ=255) cGAAAGGTCCATTAAGGTCATTTGTCATTTCGATTACTAATAAGCCTTCAAACGGCCCTTGGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATaa < 1:176514/143‑1 (MQ=255) cGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATaa > 1:213890/1‑143 (MQ=255) aaGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACAt < 1:498222/143‑1 (MQ=255) atgTCCATTATGGAGATGTGTCATATCGATAACTAATAAGCTTTCAAACGGCCCTTTGGTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATg < 2:146624/141‑1 (MQ=255) acgTCCATTAAGGACATGTGTCATATCGATAACTCATAAGCCTTCAAACGGCACTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTTAAAAGACACTTCGGCATTTATGTAAAATCAATAACATg < 2:250127/141‑1 (MQ=255) ccATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCCTGAAAAAAGCGGAAGGTGACACGACACTTCGGCATTTCCGTAAACTCAAGAACAGGaata > 2:17546/1‑143 (MQ=255) cATTAAGTACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCTCTTTTTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGattaa < 1:353432/143‑1 (MQ=255) ttAAGGACATGTGTCATTTCAATAACTAATAAGTCTTTAAACGGGACTTTGTTTTCATTATCTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGattaatt < 1:251123/143‑1 (MQ=255) ttAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACTGCCCTTAGTAAAAATTATTTGTCATTTGTAAATTCCTAGAAAAAAGCCGAAGGTCAAAAGACACTTCTGCATTTATGTAAAATCAATAACTTGattaatt > 2:64495/1‑143 (MQ=255) ttAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAGATGTTATTACGACAACTATGTAAAATCAATAACATGattaatt > 1:545790/1‑143 (MQ=255) aaGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTAt < 1:312058/143‑1 (MQ=255) aCATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGaaa < 2:449123/143‑1 (MQ=255) ttatatCGATAGCTAATACGCCTCTATACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGACTTGAAACAAGTCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCg < 1:524007/141‑1 (MQ=255) acATATCGATAACTAATAGGCCTTCAAACGGCCCTTTGTTTTCATGATTTGTCATTTGCTTATGCCTTGAAAAAAGTCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCg < 2:27946/142‑1 (MQ=255) tCGATAACTAATAAGCCTTCTCAGGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa < 2:491322/143‑1 (MQ=255) tCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGa < 2:12875/143‑1 (MQ=255) cTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGTCTTGAAAAAAGCCGAAGGTGAAAAGAGACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATaagt < 1:198647/143‑3 (MQ=255) aaGCCTTCAAACGGCCCTTTGCTTTCATTATTTTTCATTTGCATATGCCTTGAACAAAGCCGAAGGTGAAAAGGCACTTCGGCATGTATGTAAAATCAATAGCATGATTAATTAAGAAAACAGACAACTGACATAAATCcaca > 2:131473/1‑143 (MQ=255) aaGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTAGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAGTAACATGATTAATTATAAAACCAGCCGACTGACATAAATCcaca > 2:131748/1‑143 (MQ=255) aaGCCTTCAAACGGCCCTTTGCTTTCATTATTTGACATTTGCTTATGCCTTGAAAAAAGCCGAGGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGACAACTGACATAAATCcaca > 2:131433/1‑143 (MQ=255) ttCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAACTCAATAACATGATTAATTATGAAACAAGTCGACTGACATAAATCCACAAggaa > 1:24363/1‑141 (MQ=255) | GGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGA > NC_000913/2489018‑2489278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |