| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,398 | T→G | 100% | L29R (CTT→CGT) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,398 | 0 | T | G | 100.0% | 93.8 / ‑6.7 | 35 | L29R (CTT→CGT) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (19/14); minor base A (1/0); total (20/15) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG > NC_000913/3041262‑3041533 | ctctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGCCGCTGATGACGCTTGATGCCTCGGCGCGTGccac > 2:357197/1‑143 (MQ=255) ctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccacca < 1:325649/143‑1 (MQ=255) ttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTa < 2:132530/143‑1 (MQ=255) cATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCACGTCAGCCTACGGCTTCTGCCCGTTTACAGCTGACGATCATGCCACTTGATGGCTGCGCGCGTGCCACCACTACTgg > 2:264458/1‑143 (MQ=255) aGTTATTATTACTCCTTTAAGCAACTCAGTACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGcgcg < 2:156486/143‑1 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTTCTCATTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCATTCCACCATTACTGGCGCTGa > 1:66136/1‑143 (MQ=255) aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCACTCAGCCTACGGCTTCTGCCCATTTACAACTAACGCTGATCACGCCTGATCAGCGGGCCCGTGCCACCATTACTGGCGCGGAct > 2:266052/1‑142 (MQ=255) actccGCCGTTAGGCAGCTCAGGACTCTTTTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCg < 2:8351/139‑1 (MQ=255) gCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaaa > 1:462419/1‑143 (MQ=255) aGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTTGGCGCGTGCCACCATTACTCGCGCGGACAGCGAAAAAAATATGCAAGGTCAg > 1:89332/1‑143 (MQ=255) tgcctctATTTTGGCTTTTACCCCTTTTCCTCCCCGTCAGCCTCCGGCTTCTGCCCGTTTTCCGCTGACGCTGATGGCGCGTGATGACTGGGCGCGTGCCACCAGTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg < 2:346168/140‑1 (MQ=255) ccctATTGTGGCTTTTCCACCGTCTCCTCCCCGTCCGCCTACGGCTTCTGCCCGGTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGa < 2:353507/141‑1 (MQ=255) ctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAACCATATGCCGGGTCACGTGAca > 2:491942/1‑143 (MQ=255) attatGGCGTTTACACCTTTTCCTCCCCGCCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagc < 2:495063/143‑1 (MQ=255) tttgtcTTTTCCACCTTTTCCTCCCCGTCAGCCTACTGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag < 2:265136/138‑1 (MQ=255) atGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa < 1:268640/143‑1 (MQ=255) ttttACACCTGTTCCTCCCCGTCAGCCTACGGCTTCTGCCAGTTAACCGCTGACGCTAATGACGCTTGATGACTGGGCGCGTACCAGCCTTACTGGCGCCGACAGCGAACAATGGATGCATGGACAGGTGACAGCAGATGtaa > 2:142211/1‑141 (MQ=255) tttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTCCCACCATTACTGGCGCCAACACAGAAAAATAAAAGCAGGGTCAGGAGACAGCAGATGTCAg > 1:46369/1‑143 (MQ=255) ttACATCTTTTCCTGCCCGTCAGCTTACGGCTTCTGCCCGTTTACTGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCAACGATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGc < 2:2989/143‑1 (MQ=255) tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGGCAGCGAAAAATATATGCAGGGACAGGTGACAGCAGATGTCAGCCAGATGgaagaat > 1:377432/1‑142 (MQ=255) tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGACGCGTGCCACCATTACTAGAACGAAAAGCGAAAAATATATACAGGGACAAGTGACAGCAGATGTCAGCCAGATGGCagaag > 1:41592/1‑143 (MQ=255) cTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAAAAAACAAAAGAAGTGAGCAAGATGGAAGAAGATAAGCAACTg > 1:147229/1‑143 (MQ=255) cTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGAAGAGACAGGTAACAGAAGATGTCAACAAGATAGCAGAAGATCAGCACCTg > 1:218635/1‑143 (MQ=255) ccTTCTGCCCTTTTGCCGGTGATGCTGATGCTGGTTTTTGTGTGGTTGCGTGTCACCATTGCTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGTAGATGTCAGCCAGTTGGCAGAAGATCAGCACCTGCTctc < 2:301261/142‑3 (MQ=255) tttCTGCCCTTTTATCGCTGACGTTGATTACGCTTGATGACGGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgcc < 1:411433/142‑1 (MQ=255) tACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATTTGCAGGGTCCGGTGACAGCAGATGTCAGCCAGATGGCAGAAAATCAACACCTGCTCGCTGCCCATTGCGAc > 1:418250/1‑143 (MQ=255) cgctgacgctgaTGACGCTTGATGACTGGGGGAGTGCCGTCATTGCTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCTGCTGTCAGCCAGATGGCAGAAGATCAGCACATGCTAGACGCACATTGCGACGcc > 1:391482/1‑143 (MQ=255) cgctgaTCATGCTTGATGACTGGGTGCGTGCCACCATTATTGGCGCTGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGGCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt < 1:266052/143‑1 (MQ=255) gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATGTGCAGGGTCAGGTGACAGCTGATGTCAGCAAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGACAAAGGtg > 1:74704/1‑142 (MQ=255) gatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaaaa < 1:491942/143‑1 (MQ=255) cGCTTGTGGACGGTGCGCTGGCCACCATTACTGGCGCTGACAGAGAAAAATATATGCAGGGTCAGGTGACTGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTgg < 2:233036/143‑1 (MQ=255) tgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCGGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt > 1:30598/1‑143 (MQ=255) tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATGTATGCAGGGTCAGGTGACAGCAGATATCAGCCAGAAAGAAGAAGATCAGCACCTGCACGACGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATATACg > 1:190899/1‑143 (MQ=255) tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTAATAGCCGCCCATAGAGACGCCAAAGGTAAAATGTGGAGCAATTTACg > 1:8797/1‑143 (MQ=255) tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATAAGCACATGCTAGACGACAATTGAGAAGAAAAAGGTGAAATGTGGAGCAATTTACg > 1:145967/1‑143 (MQ=255) | CTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG > NC_000913/3041262‑3041533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |