| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,389,186 | T→C | 64.9% | N51S (AAC→AGC) | aaeX ← | DUF1656 domain‑containing protein AaeX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,389,186 | 0 | T | C | 64.9% | 21.2 / 13.2 | 29 | N51S (AAC→AGC) | aaeX | DUF1656 domain‑containing protein AaeX |
| Reads supporting (aligned to +/- strand): ref base T (4/6); new base C (10/7); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACG > NC_000913/3389063‑3389317 | aGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCa < 1:433215/143‑1 (MQ=255) gtccAGATTGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAg < 1:218114/141‑1 (MQ=255) gCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAAGTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGCTATCAAATTAAACAAGCAGCAATAAAGAGAGGAAAAAAACAACGCCGGAAGCCAGa > 1:38841/1‑143 (MQ=255) tGATTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTGCCACTTCAACCTCAGACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTTAACAACGCCGGATGCCAGACAAAGTc < 1:365252/143‑1 (MQ=255) tACGACCGTGATGGCCGTACGGGAGAATTTTCTCATTAGTGTTTTCACTTCAACCTCCAACGAACAGTCTCGATATCAAATAAAACAAAAAGCAAAAAAGCGCGGTGACAAACAACGCCGAATGCCAGACAAAGTCGTAGATa > 1:437687/1‑143 (MQ=255) aCGACCGTGATGTCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCACAAACGAACAGTCGCGATATCAAATAAAACCAGCAGCAATAGATCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATAc < 1:234403/143‑1 (MQ=255) cGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCACACGAACAGTCGCCATATCAAATAAAAAAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTACATAcc > 1:189038/1‑143 (MQ=255) ccGTGATGGTCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGt < 1:42388/143‑1 (MQ=255) tGATGGCGTTAGAGGAGAATTTTCTTATTAGTGTTTGCAGTTCAACCCGAAACGAACAGTAGCGATATCAAATAAAACAAGCAGCAAGAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTgg < 1:470342/143‑1 (MQ=255) ggAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAACCAAGCAGCAATAGAGCGCTGTGCTGAACAACACAAGATAACAGACAAAATAAAAGATACCTGTTGGCACAAGTACCCgg > 1:288428/1‑143 (MQ=255) ggAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGCCACAAGTAccccg > 2:580460/1‑141 (MQ=255) gagaATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCCCGGTCCTAAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTATTGCCCCAAGTACCCGgc > 2:363383/1‑143 (MQ=255) tCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCGGGCGCAccagc > 2:231902/1‑143 (MQ=255) tCTTATTAGTGTTTGCACTCCAACCTCGCACGTGCAGTCGCGATATCAAATAAGACAAGCAGCCATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATTCCTGTTGGCACAAGTACCCGGCGCAccagc < 2:71296/143‑1 (MQ=255) cgtattaGTGTTATCACGTCAACCGCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCAccagcc < 1:394629/141‑1 (MQ=255) tgtTTTCACTTCAACCTCAAACGCACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCTGATGACATACAAAGTCGTAGATACCTATTGGCACAAGTACCCGGCGCACCAGCCAGAAAATc > 1:11556/1‑143 (MQ=255) aCTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCAAGAAAATCGCCAGTg > 2:336567/1‑143 (MQ=255) ttCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGCTGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCAAGAAAATCGCCAGTGAt > 1:278052/1‑143 (MQ=255) aCCTCAAGCGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCTGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATaaaa < 2:354992/143‑1 (MQ=255) ccTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGAGGCCAGACAAAGTCATAGATACCTGTAGGCACCAGTACCAGGCGCACCAGCCAGAAAATCGCCGGTGATATAAg > 2:270899/1‑143 (MQ=255) gTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGAATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATCAAAGCAAGCCCAAAAat > 2:532266/1‑143 (MQ=255) cgtaatatCAAATAAAATAAACAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAACGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAatat < 1:194589/139‑1 (MQ=255) cgATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTCGATACCTGTTGGCACAAGTACCCGGCGCACCAGCAAAAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCg > 1:535781/1‑143 (MQ=255) cgATATCAAAAAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCg < 2:68778/143‑1 (MQ=255) aaaTAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGACGCACCAACCAGAAAATCGCCAGTGATAACAGCAATTCAAAAAATAGCGGTGCGaa > 1:315971/1‑143 (MQ=255) aaTAAATCAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGACAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAg < 1:158153/143‑1 (MQ=255) aaTAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCTAGAAAATAGCAAGTGAAAAAAGCAATTCAAAAAATATCGGTGGGAAg > 1:114027/1‑143 (MQ=255) cagtaATAGAGTTCGGTGTTTAACAATTTCGGATGCTAGACAAAGTCGTAGATAGCTGGTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCaaa < 1:34695/143‑1 (MQ=255) agagCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCCGCCAGAAAATCGCCTGTGATAAAATCAATTCAAGATTTGTTGGGAGAAAGGACAGCCCAAACACCACg > 1:513931/1‑143 (MQ=255) | AGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCACG > NC_000913/3389063‑3389317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |