| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,184,543 | C→A | 100% | P1100Q (CCG→CAG) | rpoB → | RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0% | 75.1 / ‑6.7 | 32 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (18/13); minor base G (0/1); total (18/14) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG > NC_000913/4184404‑4184682 | cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTCCTGAACCAGcc > 2:206671/1‑142 (MQ=255)cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATACCTTACGAAGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCt > 1:492473/1‑143 (MQ=255) gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGCTGGCCGGTCGTCACGGTAACAACGGTGTAATTTCTAAGATCAACACGATCGAAGATATGCCATACGATGAAAACGGTACGCCGGTACACATCGTACTGAACCAGCTggg > 1:396015/1‑143 (MQ=255) gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTCACAAGGGTGTAATTTCTAAGATAAACAAGATAGAAGATATGACTTACGATGAAAACGGTACGCGGGTAGACATCGTACTGAACCAGCTGGGCGt > 1:231543/1‑143 (MQ=255) aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGCTCGAAGATATGCCTTACGATGAAAACGGTACACCGGTAGACATCCTACTGAACCAACTGGGCGCACCGt > 2:540835/1‑143 (MQ=255) ccGTATCTAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc < 1:207614/143‑1 (MQ=255) tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACACCGCACTGAACCAGCTGGGCGTACCGTCTCGTACCAACATCGGTc > 1:236707/1‑143 (MQ=255) accgCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt < 2:56101/140‑1 (MQ=255) aTGGCAGGGCGTGACGGGAACAAGGGTGTAATTTCTAAGAACGACCCGATCGAAGAGATGCCTTACGATGAAATCGGTGCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt < 1:151373/143‑1 (MQ=255) atgtcgtcACGGAAACAAGAGTGTAATAGCTAACATCGTCGCGAACGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa < 1:122702/141‑1 (MQ=255) cgtggTAACAATGTTGTAATTTTTTATATTAACTAGATCGAAGATAAGGCTTACGACGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt < 1:190412/140‑1 (MQ=255) aCAGGGGTGTAATTTGTGAGAGCAACCCGAGCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATg < 2:367231/143‑1 (MQ=255) tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa < 1:55193/143‑1 (MQ=255) tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGAGGGGTGCGcaa > 1:556612/1‑143 (MQ=255) aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACAGTCTCGTATGAACATAGGTCAGATAATCGAAACACACCTGGGTATGGCTGCGAAAgg > 1:480706/1‑143 (MQ=255) cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAACCACCTAGGTATGGCTACGAAAAGTATc > 1:172847/1‑143 (MQ=255) tAAGATCAACCCGATCGAAGATATGCCTTACTATGAACGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAACACCCCTGGTATGGCTGCGAAAGGTATCg < 2:197594/143‑1 (MQ=255) tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCCGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATcc > 2:541467/1‑142 (MQ=255) tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAAAGGTACGCCGGTAGACATCGTACTAAACCAGCCGGGCGTACCGTATCGTATGAACATCGGTCAGATAGTCGAAACCCACCTGGGTACCGCTCCGaaacgcacag > 2:556938/1‑136 (MQ=255) aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa < 1:448550/143‑1 (MQ=255) tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc > 2:96067/1‑143 (MQ=255) atatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACATGAGTATGGCTGCGAAAGGTATCGGACACAAAATCAACGCCATg > 1:54035/1‑143 (MQ=255) atatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCACGAAACCAACCTGGGAATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg > 1:138178/1‑143 (MQ=255) tatGTCGTACGATGAAAAGGGTACGCCGGGAGACAGCGTACTGAAGCGGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc < 2:160327/143‑1 (MQ=255) atGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATCGGCGACAAGATCACCGCCATGCt > 1:543978/1‑143 (MQ=255) ctaTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTACCGTCTCTTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc < 2:267219/141‑1 (MQ=255) gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAAACGATGAATGCCATGCTGAAAcagaaacc > 2:17409/1‑138 (MQ=255) acttcGGTAGACATTGTACTGAATCAGCTGGGCGTACCGTCTCGGATGAACAGCGGTCAGAGCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc < 1:487574/139‑1 (MQ=255) gcttgTAGACATCGTACTGACCCAGGGGGGAGTACCGTCTCGTATGGACATCGGTCAGAGCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa < 1:274356/139‑1 (MQ=255) aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc < 1:540037/143‑1 (MQ=255) gACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg > 2:125871/1‑143 (MQ=255) aCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTGGCTAAACTGCGCGAATTCATCCAg > 1:476433/1‑143 (MQ=255) | CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG > NC_000913/4184404‑4184682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |