| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 756,792 | G→T | 68.6% | E296* (GAA→TAA) | sdhA → | succinate:quinone oxidoreductase, FAD binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 756,792 | 0 | G | T | 68.6% | 6.6 / 14.0 | 23 | E296* (GAA→TAA) | sdhA | succinate:quinone oxidoreductase, FAD binding protein |
| Reads supporting (aligned to +/- strand): ref base G (3/4); new base T (5/11); total (8/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-01 | |||||||||||
GCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCG > NC_000913/756655‑756932 | ccGTACTGGTCACCGAAGGTTGCCGTGGCGAAGGCGGTTATCTGCTGAACGCACATGGCGAACGTTTTATGGAGCGTTATGCGCAGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATc < 2:277842/142‑1 (MQ=255) gAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCAGGCAGGCCGTAACGTAGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCgg > 1:302128/1‑143 (MQ=255) cgTTGGCGTGCTGAAGGCGGGTAGCAGGGGAACAAACATGGCGAACGTGTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCtg < 1:329991/142‑1 (MQ=255) tGCCGTGGTGAAAGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATACGCCGAACGCCAAAGGCCTGGCGGGCCGTCACGTGGTTGCGCGTTCCATCATGATGTAAGACCGACAAGGGCGCGGcggcgg > 2:464964/1‑138 (MQ=255) cGGTTATCTGTTGAACAAATTTGGCGAACGTTTTTTGGAGCGTTATGCGCGTAACGCCAAAGACCTGGCGGGCGGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGc < 1:455531/143‑1 (MQ=255) gggtagctgcggAACAAGCGGGGGGAACTTTTGCTGGAGCGTTATGCGCCGACCTCCAAAGGCCTGGCGGGGCGTGCCGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGcc < 2:516242/137‑1 (MQ=255) gTTATCTGCTGAACAAACATGGCGAACGTTTTATGGTGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGGTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCca < 1:331134/143‑1 (MQ=255) gctgAACAAACATGGCTAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTGCATCATGATCTCAATGCGTTAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCga < 1:550733/143‑1 (MQ=255) tgAACAAACATGGCGAACGTTTTATGGTGCTTTTTGTGTTGATCTTTTAAGTTCTGGTGGGCCGTGACGTGGTTGCGCGGGCCAGCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaa < 1:158089/143‑1 (MQ=255) aaaccgggCGAACGTTTTGTGGAGTGGTATGCGCCGAACGCCAGCTACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCAGGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactgaa < 2:180729/137‑1 (MQ=255) gAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCCTGATCTAAATCCGTTAAGGTCGAGGCTGTGATGATCCGTGAGGGAAAAACGCaaaacaaaaacaatataa > 1:70618/1‑136 (MQ=255) gAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCAGGCGGTCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCAGTGGGGGGCACACGCgaaactgaaactgcaaca > 2:567067/1‑137 (MQ=255) accGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGTGGGTCGTGACGTGGTTGCGCGTTTCATCATGATCTAAAGCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCAc < 1:105792/141‑1 (MQ=255) ttttATGTAGCGTTATGCGCCGAACGCCAAAGTCCTGGCGGGCCGGGACGTGGTTGCGCGTTCCATCATGAGCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTgg < 1:345592/143‑1 (MQ=255) tATGGAGCGTTATGCGCCGAACGCCAAAGTCCTGTTGGGTCGTGATGTGGTTGCGCGTTCCATCATGATCTAAAGCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTa < 1:540974/143‑1 (MQ=255) ggAGCTTTATGCGCCGAACGCCAAAGTCCTGGCGGGTCGTGACGTGGTTGCGCGTTCCATCAAGATCTAAATCCGTGAAGGGCGCGGCGGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaag < 1:375912/143‑1 (MQ=255) ccGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATTGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATc < 1:450980/143‑1 (MQ=255) ggCCGTGATGTGTGTGCGCGTTCCATCATGATTTTACTCTTTGTAGGTCTCTGTTGTGATGGTCCGTTGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGa < 1:273583/143‑1 (MQ=255) gTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGCACAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGAAGCTTTCCCg > 2:63580/1‑143 (MQ=255) ggTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGACACTGAAACTCGATCACCTGGGTAAAGAAGATCTCGAATACCGTCTGCCGGGAATCCTGGAGCTTTCCCGTa > 1:336071/1‑143 (MQ=255) gcgTTCCATCATGATCTAAATCCCTTAAGTTCGCGGCTGTGGCGCGCCCAGAGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg < 1:413494/143‑1 (MQ=255) tCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAACCTGAAACTCGATCACCTGGGTAAAGAAGTTATCGAATACAGTCTGCCGGGTAGCCTGGAGCTTTCACGTACCTTCGCTCACGTCGATCCg > 1:491280/1‑143 (MQ=255) tCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGGCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCACTCACGTCGATCCg > 2:519158/1‑143 (MQ=255) | GCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCG > NC_000913/756655‑756932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |