Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,232	C→T	100%	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,232	0	C	T	100.0%	29.5 / ‑6.7	15	T370T (ACC→ACT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/7);  minor base G (0/1);  total (7/8)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGA  >  NC_000913/1196097‑1196311                                                                                                                                        |                                                                                tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCTGATATTTGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATCCTCGCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCgg                                                                          <  1:417196/143‑1 (MQ=255)  gCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCTACCCACGGTACTGCGCCGAAATATGACGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGc                                                                         <  1:439554/143‑1 (MQ=255)        aTCGGTGACGACCGCGCCCTGCTTGACGCCTCCCACGGTTCTGCGCTTAAATATGCCGGTCAGTACATACTAGATCCTGGCTTTATTATTCTCTCCGCTGAGATGATGCCGCGCCAATTTGCGTGGTTTGAAGCGGCTGAcct                                                                   <  2:278595/143‑3 (MQ=255)            gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTATATCAAATGAGATAATACTGCGCCATATGGAATGGACTGAAGCGGCTGACCTGAtt                                                               >  1:4549/1‑143 (MQ=37)            gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAAACCTGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgatt                                                               <  1:500537/143‑7 (MQ=38)              gACGAACGCGCCCTGTTGGAAGCCACCCACGGTACTGCGCCGAAAGATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTAGTCGCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGt                                                             <  1:371937/143‑1 (MQ=37)                       gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGTACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                    <  1:295938/143‑1 (MQ=37)                                 aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGTCTCTATTATTCTCTCCTCTGAGATGATTCTGCGCCATATGGGTGGGACTGAAACGCCTCACCTGATTGTAAAAGGAATGCAAGGCGCa                                          >  1:167499/1‑143 (MQ=255)                                       cacacTGTACTCCGCCGAAATTTGCCGGTCAGGACAAAGTAACTCCTGGCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                    <  2:249990/141‑2 (MQ=21)                                                      cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCCAACAATGCCAAGACCGTAAct                     >  2:556030/1‑142 (MQ=18)                                                        aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTTTCCTCTGAGATGATGCTGCGCCAGATGGGATGGACGGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa                   <  1:64429/143‑1 (MQ=17)                                                             tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGTGGCTAACCTGATTGATAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt              >  1:280524/1‑143 (MQ=17)                                                                  gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAATTCGaa         >  2:413165/1‑142 (MQ=14)                                                                   tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGCTGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGGATGGAAGGCGCAATCCATGCCAAGACCGTAACTTATGACTTccaac        >  2:444172/1‑139 (MQ=14)                                                                         cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTCTGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCAAACGTCTga  >  1:536987/1‑143 (MQ=14)                                                                                                                                        |                                                                                TGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGA  >  NC_000913/1196097‑1196311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑