Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,247	A→G	100%	L375M (TTA→CTG)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,247	0	A	G	100.0%	16.7 / NA	11	L375M (TTA→CTG)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/4);  total (7/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGA  >  NC_000913/1196108‑1196311                                                                                                                                            |                                                                 gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTATATCAAATGAGATAATACTGCGCCATATGGAATGGACTGAAGCGGCTGACCTGAtt                                                               >  1:4549/1‑143 (MQ=37)   gACGAACGCGCCCTGTTGGAAGCCACCCACGGTACTGCGCCGAAAGATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTAGTCGCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGt                                                             <  1:371937/143‑1 (MQ=37)            gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGTACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                    <  1:295938/143‑1 (MQ=37)                      aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGTCTCTATTATTCTCTCCTCTGAGATGATTCTGCGCCATATGGGTGGGACTGAAACGCCTCACCTGATTGTAAAAGGAATGCAAGGCGCa                                          >  1:167499/1‑143 (MQ=255)                            cacacTGTACTCCGCCGAAATTTGCCGGTCAGGACAAAGTAACTCCTGGCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                    <  2:249990/141‑2 (MQ=21)                                           cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCCAACAATGCCAAGACCGTAAct                     >  2:556030/1‑142 (MQ=18)                                             aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTTTCCTCTGAGATGATGCTGCGCCAGATGGGATGGACGGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa                   <  1:64429/143‑1 (MQ=17)                                                  tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGTGGCTAACCTGATTGATAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt              >  1:280524/1‑143 (MQ=17)                                                       gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAATTCGaa         >  2:413165/1‑142 (MQ=14)                                                        tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGCTGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGGATGGAAGGCGCAATCCATGCCAAGACCGTAACTTATGACTTccaac        >  2:444172/1‑139 (MQ=14)                                                              cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTCTGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCAAACGTCTga  >  1:536987/1‑143 (MQ=14)                                                                                                                                            |                                                                 GTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGA  >  NC_000913/1196108‑1196311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑