| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,085 | Δ1 bp | coding (2069/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | . | 95.2% | 164.6 / 1.6 | 43 | coding (2069/2664 nt) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (25/15); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124947‑125221 | ctCACAGCCACATTCAGCCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTa < 2:259283/142‑1 (MQ=255) tCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTCGGTACGGTGAAAAACAAGAGAACGTTTGCTACTACA‑CACTAc > 2:362069/1‑143 (MQ=255) aGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGAGTGACCGCCCGCAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTg > 1:714894/1‑143 (MQ=255) ccACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGGCCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTAC‑GCACTACGCTgca > 1:967368/1‑141 (MQ=255) tCAGTCGCTGTCTGTCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaaaa < 2:99739/143‑1 (MQ=255) gTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTa > 1:602024/1‑143 (MQ=255) gcggACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACca < 1:598670/140‑1 (MQ=255) cTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATAATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACcac > 1:224576/1‑143 (MQ=255) gACTATCCGGAACTGGATCTCTTCCGACCCGGCCTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACAt < 2:762592/143‑1 (MQ=255) tCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCgg > 2:218764/1‑143 (MQ=255) cccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGc > 1:235079/1‑143 (MQ=255) cGAACTGCATCTCATACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCaa < 1:451278/143‑1 (MQ=255) cgctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTAGCGCGTATGTACGATGAAACAGAAGAGCACGTTTACGACTACA‑CACTACGCAGAACAAAGACTACCACATGCCGGCAATGCCGGAAgg > 2:147074/3‑143 (MQ=255) ttctgACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTTAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGc < 2:851649/139‑1 (MQ=255) ttACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCCGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGc < 1:379057/143‑1 (MQ=255) cgccttgcggttacgAAGTGGCGGTCATGGTGCATGACGGGCTGGAGCGTATGGACGGTGAAAAAGAAGAGAGCGTTGACTACGACA‑CGCTACGCTGAACGAAAACTACCAGATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 2:416477/140‑1 (MQ=255) ttacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAACCTACAATATGGCTGTAATGCCGGAGGGTGCTGGGGCAGGGAt > 1:662762/1‑143 (MQ=255) acgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATcc > 1:660539/1‑143 (MQ=255) gcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGCGATGAGGAAGGTATCCGt > 2:681466/1‑143 (MQ=255) ttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaa > 1:319276/1‑143 (MQ=255) gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTAAGGAAGGTATCCGTAAAGGt > 1:840167/1‑143 (MQ=255) gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGAGCTGAGGAAGGTATCCGTAAAGGt > 1:849400/1‑143 (MQ=255) aGTTGCTGTCATCATGCATGACGGGCCGGAGCGTATGTACGGTGAAACACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTAt < 1:158556/143‑1 (MQ=255) tGCTGTCAGCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTa < 1:847356/143‑1 (MQ=255) gCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAACGGTATCTAc > 2:957706/1‑143 (MQ=255) gTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGGCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaaa < 2:17005/143‑1 (MQ=255) catcatGCATGACGGCCGGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACt < 1:628919/143‑1 (MQ=255) atgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTGTCCGTATCGGTATCTACAAATTCGaa > 1:233297/1‑143 (MQ=255) gcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAAAGCCGGAAGGAGCTGAGGAAGGGATCCGTAAAAGAATCTACAAAccgggaac > 2:808972/1‑136 (MQ=255) tattgcGGTCTGGAGCGTATGCACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTGCGCTGAACGAAAACGACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACt < 2:406090/138‑1 (MQ=255) gACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCCGCAATGCAGAAAGGTGCTGAGGAAGGTATCCGTAAAGGTCTCTACAAACTCGACACtgcc > 2:866430/1‑140 (MQ=255) tCTGGAGCGTACGTACGGTGAAAAACAAGAGAACGCTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGGATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAgg < 2:801593/143‑1 (MQ=255) gAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGc > 1:155048/1‑143 (MQ=255) gCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaa > 2:938146/1‑143 (MQ=255) gTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAg < 1:769514/143‑1 (MQ=255) tATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGACGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAGGGGTATCTACAAACGCGAAACTATGGAAGGGAGCAAAgg > 2:89631/1‑143 (MQ=255) cgTGAAAAACAAGAGAACTTTTACTTCTA‑TTCTCTTCTCTGAACAAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGtt < 1:410676/142‑1 (MQ=255) aaaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACGCGAAACTGTTGAAGGTAGCAGAGGTAAAGTTGAgct > 1:422774/1‑143 (MQ=255) aaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGCGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgccgc > 2:681204/1‑143 (MQ=255) ctagcgaACGTTGAGTAATAC‑TCACTAAGGTTACCGAAAACCACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGCTATCCGTAAATTTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTc < 2:25838/138‑1 (MQ=255) gaACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGCAGGTGCTGCGGAAGGTATACGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGAAAAGGTAAAGTTCAGCTGCTCGGCTc > 1:460771/1‑143 (MQ=255) cGTTTACTACTACA‑CACTACAGAGAACGATAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCgg > 2:859316/1‑143 (MQ=255) tactacA‑CACTACGGTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCCAtg > 1:700470/1‑142 (MQ=255) | GTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124947‑125221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |