| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 97.3% | 105.4 / NA | 37 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (16/20); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132651‑2132926 | cTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCg > 2:245863/1‑143 (MQ=255) ggTAGAGGGTAAACGGCTGATAGTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTc < 1:197511/143‑1 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTC‑TGCGTTGc > 1:171613/1‑143 (MQ=255) agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCctc > 1:602983/1‑143 (MQ=255) gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCctct > 1:483515/1‑143 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACACGGAGAATTTTTTCGGTGACACAGTGATATACTTTTCACGGTGATTGATATGCAGGCTC‑TGCGTGGCCtctt > 2:335973/1‑143 (MQ=255) gTAGACAGCTGGTATTTTTTGCTGTGGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCtcttc < 2:577738/143‑1 (MQ=255) cAGCTGAGATCTTGTCCGGGCGCGGCCGAATCTGTAAATGTGCGTGCGTTATATGTGGAAGTACCCGGCGAATTTTGTCGGCGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACtt < 1:550549/143‑1 (MQ=255) aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGC‑GGACCGTGCGTCGCCTATTCTACttt > 2:510967/1‑143 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGtt > 2:155440/1‑143 (MQ=255) tgttGCTGGCGAGGTCGAATTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCg < 1:250760/141‑1 (MQ=255) gcgcGGTCTTATTTGTCTTTTTGTTTGCTGTAAAATTGGAAGTAGCCGGAGAATTTGTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc < 1:299685/143‑1 (MQ=255) cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCGACGGTTCGCCGTACTcc > 2:879752/1‑143 (MQ=255) cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTcc > 2:411032/1‑143 (MQ=255) cgcgGTCCACTTTCTCTTTGTGCTTGCGGTAAAGTGGGATGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCACGTGATTTATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc < 2:958444/143‑1 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa > 1:910436/1‑143 (MQ=255) ttcccGGAGTATTTTTTCGGTGACGGGGTGATCTGCATTTCACCTTGATTGATAGGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt < 2:323786/141‑1 (MQ=255) gtatacttttttCGGTGACGATGTCATCTGCATTTCTCCGTGTTTGATATGCACGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt < 1:418755/137‑1 (MQ=255) ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCCTTCAttt > 1:542682/1‑143 (MQ=255) gagaATGTTGTCTGTGTCGGGGGTGTCTGCATTTCACCCGGATTGTTATGCAGGCTC‑TGCGTCGCTGTTTCTACTTTCCACGGTTCTCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc < 2:306098/143‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCGGGCTC‑TGCTCCTCCGCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 1:447957/143‑1 (MQ=255) ttgtCGGTGACGGGGTGATCTGCATTTCACCGTGATTGAGATGCAGGCTC‑TGCGTCGGCTCTTCTACTGGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 2:228439/140‑1 (MQ=255) gggatgAGGTGATCTGCACTTCACCGTGATTGAAATCCAGGCTC‑TGCGTCGCCTCGTCTACTTTCCACGGTTCGCCGAACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 1:415504/138‑1 (MQ=255) aGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct < 2:602983/143‑1 (MQ=255) cgtgggcTGCTTTTCTCCGTGACTGCTATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt < 2:973533/137‑1 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGCACTCCTGTGCCGCTTTCAGTTCGGTATCGAACAACCACCCTTTGaacc > 2:77611/1‑141 (MQ=255) gCATTTCACTGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTATGAAACGc > 1:773957/1‑143 (MQ=255) cATTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc < 2:483515/143‑1 (MQ=255) aTTTCACCGTGATTGATATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGCAACCCCa > 2:226934/1‑143 (MQ=255) cGTGCTTGATATGCAGGCTC‑TGCGCCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg < 1:379214/143‑1 (MQ=255) gattgatATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTa < 1:155440/143‑1 (MQ=255) attgatATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg > 2:566954/1‑89 (MQ=255) attgatATGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg < 1:566954/89‑1 (MQ=255) atatGCAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCCCTTTCAGTTCGGTCTCGAACAACCACCCTTTGAAACGCCACGCCCCGGTACAGAc > 1:163801/1‑143 (MQ=255) gCAGGCTC‑TGCGTCGCCTCCTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg < 1:584950/143‑1 (MQ=255) cAGGCTC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCACCGCAAGATGTCGTAATCCTGTGCCGCTTTCAGTACGCTATCGAACACACACTCTTCGCAACCCCATGCCCAGGTAAACACCTgac > 2:532034/1‑141 (MQ=255) aGGCTC‑TGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt < 1:332038/143‑1 (MQ=255) | CTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATC‑TGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132651‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |