| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,489,152 | C→T | S6N (AGC→AAC) | yfdE ← | acetyl‑CoA:oxalate CoA‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,489,152 | 0 | C | T | 96.9% | 95.5 / NA | 32 | S6N (AGC→AAC) | yfdE | acetyl‑CoA:oxalate CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (16/15); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACA > NC_000913/2489021‑2489290 | tATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTAttt < 1:898016/143‑1 (MQ=255) cATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATACTTCATAAAGAAAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGc > 1:361418/1‑143 (MQ=255) tttaCCCGTCGTCTCATCTGTATTTACCGTTTCGCCCATATTACAAAGAAGTTGAGTTCCGATAGGTCCATTAATGACATGTGTCATATCGATAACTAATAAGTCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCt < 2:86162/140‑1 (MQ=255) ccGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCGTTTGTTTTCATTATGTGTCATTTGCGTGTGc > 2:25707/1‑143 (MQ=255) cggcggCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGCAGTTGAGTTCCGAAAGGTCCATTAAGGACGTGTGTCATATCGATGACTAATAAGTCTTCACACGGCCCTTTGTGTTCAGTATTTGACATTTGCTTATGct > 2:25666/1‑142 (MQ=255) ggcTCAACTTTAATTCCCCTTGCGCCTATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTAATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGa < 2:906786/143‑1 (MQ=255) caccgttAATTACCCTTGCGCCCATATTACAAAGAAGTCGAGTTCCGAAAGGTCCATTCAGTACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGaaaaa < 1:361455/138‑1 (MQ=255) tttAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCGTTATTTGTCATTTGCTTATGCATTAaaaaaacca > 1:233013/1‑140 (MQ=255) atccattGCTCCCATATTACATAAAAATTGAGTTACTAAAGGTCAATTAATGACATGTGTCATATCGATAACTAATAAGCCTTCAAAAGGCCCTTTATTTTCATTATTTGTCATTTACGTATGCCTTAAAAAATGCCAAAAGt < 2:501383/138‑1 (MQ=255) ttGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGaaaa > 1:529230/1‑143 (MQ=255) atTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAACCGCAGGTGAAAAGACACTTCgg > 1:931478/1‑143 (MQ=255) cAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCAtt > 1:280702/1‑143 (MQ=255) aaaGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGCATCATATAGATAAATAATAAGCATTCAAACGGCTCTATGCTTTCATTATTTATCACTTGCTTATGCAATGAACAACGCAGACGGTAGAAAAACATTTAGGCAttc > 2:95304/1‑142 (MQ=255) agaagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTAt > 2:593997/1‑143 (MQ=255) aGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGGAGGTGAAAAGACACTTCGGCATTTATGTAAAATc > 1:493424/1‑143 (MQ=255) ttCCCAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCTAACGGCCCTTTGTTTGCATTATTTGTCATTTGTTTATGCCTTGAAAAAAGCCTAAGGCGAAAAGACACTTCGGCATTTATGTAAAATCaa < 2:511524/143‑1 (MQ=255) cGAAAGGTCCATTAAGGACATGTGTCATATCGATCACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAACAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCactca > 1:324793/1‑139 (MQ=255) ggTCCATTAAGGACAGGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGGCATTTGCTTATGCCTTTAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGa < 2:64549/143‑1 (MQ=255) gTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTTAAAAAAGCTGTAGGTGAACAGACACTTCGGCATTTATGTAAAATCAATACCATGat > 2:951495/1‑143 (MQ=255) aTTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGattaat < 2:531523/143‑1 (MQ=255) ggACATGTGTCATATCGATAACTAATAAGCCTTCACACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCATTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGattaagtaata > 2:322697/1‑140 (MQ=255) ataTGTGTCATATCTATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGaaa < 1:118065/141‑1 (MQ=255) gtgtCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAg < 2:187077/143‑1 (MQ=255) tatCGCTAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATACCTTGAAAAAAGCAGCAGGTGAAACGACACTTCGGCATTTATGTAACGTCAATAACATGATTAATTATGAAATCAGTCGACt > 2:872171/1‑143 (MQ=255) cGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGAc < 1:593997/143‑1 (MQ=255) ctaagcaGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAAt < 1:172300/137‑1 (MQ=255) ccaataaGCCTTCACACGCCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAAt < 1:912126/141‑1 (MQ=255) atgtaGCCTTAAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCAACTGACATAAATcc < 2:373866/139‑1 (MQ=255) aGCCTTCAAGCGGACCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGAAACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACaa < 2:368563/143‑1 (MQ=255) ccTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCGACAAgg > 2:493686/1‑143 (MQ=255) ttCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAGAGACACTTCGGCATTTCTGTAAAATCAGTAACCTGATTAATTATGAAACCAGTAGACTGACATAAAGTCACAAGGGa > 2:849127/1‑143 (MQ=255) tttGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGAAGCAGTGACGACa > 1:337005/1‑143 (MQ=255) | TATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACA > NC_000913/2489021‑2489290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |