Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	146.2 / NA	49	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/23);  total (26/23)

ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTT  >  NC_000913/3041258‑3041530                                                                                                                                             |                                                                                                                                     aTAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGGTGATTGGGCGCGTg                                                                                                                                    >  1:357344/1‑143 (MQ=255)        ttttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGTCGCTGCTCACGCTTGATGACTGGGCGCGTGccaccac                                                                                                                             >  2:417183/1‑142 (MQ=255)        ttttACCAGTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTGTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAt                                                                                                                             <  1:27670/143‑1 (MQ=255)          ttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATCATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGGGTGccaggattg                                                                                                                           >  1:830468/1‑137 (MQ=255)             ccATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGTCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGCTCACTGGGCGCGTGCCACCAtccctc                                                                                                                        >  2:447997/1‑138 (MQ=255)                    tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                                 <  1:347294/143‑1 (MQ=255)                      aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTTCACATTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGATGAAAACACTTGATTACCGGGCTCGTCCCGCCATTACCGGCGCGGagg                                                                                                               >  2:525622/1‑141 (MQ=255)                       ttAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAg                                                                                                              >  1:92180/1‑143 (MQ=255)                                cGTTAAGCAACTCAGGACTCGATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCACCATCACTGGCGCGGACAGCGAAGAata                                                                                                     >  2:860736/1‑143 (MQ=255)                                  ttAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatata                                                                                                   <  2:417760/143‑1 (MQ=255)                                    aaGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGCGCCACCATTACTTGCGCTGACACCGGAAAatacact                                                                                                 >  2:445444/1‑139 (MQ=255)                                       cAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTCCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAg                                                                                              <  1:373096/143‑1 (MQ=255)                                         aCTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAggg                                                                                            <  1:673377/143‑1 (MQ=255)                                             aGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg                                                                                        >  1:792046/1‑143 (MQ=255)                                               gACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAAGATATGCAGGGTCAGGt                                                                                      >  2:117878/1‑143 (MQ=255)                                                  tctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgggggc                                                                                   >  1:541206/1‑139 (MQ=255)                                                  tctATTATAGCCTTTACACCTGCACCGCCCCGTCAGCCTATGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc                                                                                   <  1:607684/143‑1 (MQ=255)                                                    tattatGGCTTTTACACCGTTCCCTCCCGCTCAGCGGACGTCGTCTGCCCGTTTCCCGCTGACGCTGATGGCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACATCGAAACACCTATGCAGAGTCAGGTGAcag                                                                                 <  2:700458/143‑1 (MQ=255)                                                      ttctGGCTTTTACGCCTTTTCCTCCCTGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGTTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagca                                                                               <  2:610935/140‑1 (MQ=255)                                                      ttatGGCGTTTACACCTGTCCCTCCCCGTCAGGCTACGGCTTCTGCCCGTTGACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagca                                                                               <  2:221275/143‑1 (MQ=255)                                                        atGGCTTTTACTCCTTGTCCTCCCCGTCAGCCTACGGCTTCGGCCCGTTTACCGTCGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAGTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa                                                                             <  2:925588/143‑1 (MQ=255)                                                            cTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGCAAAAGAGAGGCAGGGTCAGGTGACAGCAGATGTc                                                                         >  1:728901/1‑143 (MQ=255)                                                              tttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACTGCGAAAAATATCTGCCGGGTCAGGTTGCCGCGGATGTCAg                                                                       >  1:301567/1‑143 (MQ=255)                                                              tttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCCGACAGCGCACAACATATGCAGGGGCAGGTGCCCGCAGATGCCAg                                                                       >  2:659211/1‑143 (MQ=255)                                                                tACACCTTTTGCTCCCCGTCAGCCTACGGCCTCTGCCCGCTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGcc                                                                     <  2:41705/143‑1 (MQ=255)                                                                   acCGTTTCCCCGCCGTCGACCTCCTTCTTCTGCCCGTTTCCCGCTGACGCTGATGACGCTTGATGACTTGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGa                                                                  <  2:333860/143‑1 (MQ=255)                                                                          cccccccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCaga                                                           <  1:172152/140‑1 (MQ=255)                                                                            tccCCGTCGGCCTACGGCTTCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                                                         <  2:774318/143‑1 (MQ=255)                                                                            tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATCCATGCAACGTCAGGTGACAGCAGTGGTCGGCCAGATGGCagaag                                                         >  1:445187/1‑143 (MQ=255)                                                                             ccccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCCGGTGCCGGCAGATGTCAGCCAGATGGCagaaga                                                        >  1:505603/1‑143 (MQ=255)                                                                              cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGAGGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAt                                                       <  1:385843/143‑1 (MQ=255)                                                                                        gcctgCTTCTGCCCGTTGTCCGCTTACGCTGATGTCGCTTTATGCCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGCGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGc                                             <  2:213885/139‑1 (MQ=255)                                                                                                 tGCCCTTTTACCGCTGACGCTGATGACGCTTGATGACTCGTCGCGTGCCACCATTACTGGCGCGCACAGCGATAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc                                    <  2:293331/143‑1 (MQ=255)                                                                                                      gTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt                                                                 >  1:44777/1‑109 (MQ=255)                                                                                                      gTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt                                                                 <  2:44777/109‑1 (MQ=255)                                                                                                        ttACCGCTGACGCTGATGACGCTTGATGACTGGGCTCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATCGCGa                             >  2:859673/1‑143 (MQ=255)                                                                                                          aCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGCCg                           >  2:373172/1‑143 (MQ=255)                                                                                                             gctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgcc                                        >  2:408021/1‑127 (MQ=255)                                                                                                             gctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgcc                                        <  1:408021/127‑1 (MQ=255)                                                                                                              ctgacgctgaGGACGCTGGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaa                       <  1:397420/143‑1 (MQ=255)                                                                                                                   gctgaTGACGCTTAATGACTGGGTGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGCCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTa                  <  2:318434/143‑1 (MQ=255)                                                                                                                      gagcaCGCGTGGTGGCTGGGCGCGTGCCACCATGACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCTCATTGCGACGCCAAAGGTaaaa               <  1:72182/139‑1 (MQ=255)                                                                                                                       atgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGCACAGCGAAAAATAAATGCAGGGACAGAAGACAGCAAAAGTCAGCCAGATGGCAGAAGATCAGCACCTGCGCGCCGCCCATTGCGACGCCAAAGGTAAAAt              >  1:311676/1‑143 (MQ=255)                                                                                                                            gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGCTCAGGTGACAGCAGCTGTCAGCCAGATGGCAGAAGATCCGCACCTCCTCCCCGCCCCGTGCGCCGCCAAACGCAAAACGCGGa         >  2:761373/1‑143 (MQ=255)                                                                                                                             cTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAg        >  1:532948/1‑143 (MQ=255)                                                                                                                              ttGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCGCGCCGCCCCTTGCGACGCCAAAGGTAAGATGTGGAGc       >  1:107863/1‑143 (MQ=255)                                                                                                                                   gaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCATGTGACAGCAGATGTCACACAGATGGCAGAAGATCCGCACATGCTCGCCGCCCATTGCAACGCCAAAGGTAAAATGTCGAGCaagcg  >  2:391378/1‑140 (MQ=255)                                                                                                                                   gaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGGGCAAttt  >  1:305583/1‑143 (MQ=255)                                                                                                                                   gaCGGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAAGATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAttt  <  2:244367/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                     ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTT  >  NC_000913/3041258‑3041530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑