Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,389,186	T→C	N51S (AAC→AGC)	aaeX ←	DUF1656 domain‑containing protein AaeX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,389,186	0	T	C	100.0%	142.6 / NA	50	N51S (AAC→AGC)	aaeX	DUF1656 domain‑containing protein AaeX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/27);  total (23/27)

ATTAAAAATTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCAC  >  NC_000913/3389046‑3389316                                                                                                                                             |                                                                                                                                   aTTAAAAATTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTg                                                                                                                                  <  2:130157/143‑1 (MQ=255)  ttAAACATTCCGATGAAGGCCAGAATGATTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGGGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGa                                                                                                                                 <  2:144745/143‑1 (MQ=255)     aaaaTTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGaaca                                                                                                                              >  1:842836/1‑143 (MQ=255)                    gCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGa                                                                                                               <  1:347157/143‑1 (MQ=255)                    gCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGa                                                                                                               <  1:358314/143‑1 (MQ=255)                    gCCACAATGACTAACACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGa                                                                                                               <  1:368462/143‑1 (MQ=255)                     ccAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCGCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGAc                                                                                                              <  1:73319/143‑1 (MQ=255)                              cTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTa                                                                                                     <  2:88946/143‑1 (MQ=255)                              cTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTa                                                                                                     <  1:406788/143‑1 (MQ=255)                               tAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAg                                                                                                    >  2:750645/1‑143 (MQ=255)                               tAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGGTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAAAGGGCGGTGCTGAACAACGCCGGATGCCAGACAGAGGCGGAg                                                                                                    >  2:647552/1‑143 (MQ=255)                                 aTACGACCGTGATGGCCGTACGGGAGAATTTTCAAATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAAAGCGCGGGGCTGAACAAGCACAGATGCCAGACAAAGACGGAggc                                                                                                  >  2:447179/1‑141 (MQ=255)                                   aCGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGTGATATCAAATAAAACAAGCAGCAATAGAGAGCGGTGCTGAACAACGCCGGATGCGAGACAACGTCGAAGATaa                                                                                                >  1:110633/1‑142 (MQ=255)                                          gaagggCCGTAGGGGGGGATTTTCGTATTAGTGTCGTCACTTCAACGTCCACCGAGCAGTCGCGATATCAAATAAAACGAGCAGCAAGAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTgg                                                                                         <  2:642519/139‑1 (MQ=255)                                             tGGCCGTACGGGAGAACTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACCAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGcac                                                                                      <  1:35697/143‑1 (MQ=255)                                               ggcGTACGGGAGAATTTGCTTATTAGTGGTGTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACaa                                                                                    <  1:456345/141‑1 (MQ=255)                                               gCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGCACAAGTCGTAGATACCTGTTGGcacac                                                                                    >  1:45724/1‑142 (MQ=255)                                                 cGTACGGGAGAAGTTTCTTATTAGTGTGTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAACACAAGCGTCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATTCCTGTTGGCACAAGt                                                                                  <  1:196712/143‑1 (MQ=255)                                                    aCGGGAGAATTTTCTTATTAGTGTGTTCACTTCAACCTCATACGAACAGTCGCTATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTAcc                                                                               <  2:277392/143‑1 (MQ=255)                                                        gagaATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACACCGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGTCACAAGTACCCggg                                                                           >  1:832249/1‑142 (MQ=255)                                                          gaATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGTACAAGTACCCGgcgc                                                                         <  2:129840/143‑1 (MQ=255)                                                            atgttCTTACTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCAc                                                                       <  1:473875/140‑1 (MQ=255)                                                             ttttCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGAAATAGAGCGCGGTGCTGAACAACGACGGATGAGAGACAAAGTCGTAGATAACTGTTGGCAAAAGTACACGGGGCaac                                                                      >  2:357035/1‑141 (MQ=255)                                                              tttCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACACCGCCGGATCCCAGACAAAGTCGTCGATACCTGTTGGCGCGAGTACCCGGCGCAcag                                                                     >  2:775166/1‑141 (MQ=255)                                                              tttCTTATTAGTGTATTCACTTCAACCTCAAACGAACTGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCAcca                                                                     <  1:513246/143‑1 (MQ=255)                                                               ttCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCAacag                                                                    >  2:326277/1‑143 (MQ=255)                                                                ttgtattaGTGTTTTCACTTCAACCGCAAACGAGCAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCAccagc                                                                   <  2:683481/140‑1 (MQ=255)                                                                tCTTATTAGTGTTCTCACTTCAACCTCACACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACACCGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCAccagc                                                                   <  1:56598/143‑1 (MQ=255)                                                                        gtgtTTTCACTTCAACCACAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGAGGTGCTAAACAACGCCGGATGCCAGACAAAGTCGTAGATCCCTGTTGGCACAAGTACCCACCGACCCAGCCAGaaaac                                                           >  2:253573/1‑142 (MQ=255)                                                                         tgtTTACACTTCAACCGCAACCGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGCATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATc                                                          <  1:750100/143‑1 (MQ=255)                                                                           ttttCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGc                                                        >  1:660067/1‑143 (MQ=255)                                                                             ttCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAGATCGCCa                                                      >  2:114820/1‑143 (MQ=255)                                                                             ttCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCa                                                      <  1:639389/143‑1 (MQ=255)                                                                             ctCACTTCATCCTCACACGAAGGTTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGCACACCGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCa                                                      <  2:24128/142‑1 (MQ=255)                                                                               cACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACACGTACCCGGCGCCCCAGCCCGAGCATCGCCAgc                                                    >  1:350316/1‑142 (MQ=255)                                                                                  ttCAACCTCAAACGAACAGTACCGATATCAAATAAAACAAGCAGCAATAGAGCGCGATGCTGAACCACGCCAGATGCCAGACAAAGTCGTAGATACCTGTTGGCCCAAGTACACGCAGCACCAGCCAGAAAAACGCCAGtagt                                                 >  2:226779/1‑140 (MQ=255)                                                                                         tCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCa                                          >  1:521392/1‑143 (MQ=255)                                                                                                   aGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCaaaaaa                                <  1:203948/143‑1 (MQ=255)                                                                                                         gATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCCAAAAATCTCgg                          >  1:396444/1‑143 (MQ=255)                                                                                                         aatagCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAAAAACGCCGGATGCCAGACAAAGTGGTAGATACCTGTAGGAACAAGTACCAGGCGTACCAGTAAGAAAATCGCAAGTGATAAAAGCAATTCAAAAAATATCgg                          <  2:791252/142‑1 (MQ=255)                                                                                                               aaaTAAAACAAGGAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGaa                    <  2:903665/143‑1 (MQ=255)                                                                                                               aaaTAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATAGCGGtagcaa                    >  2:146419/1‑138 (MQ=255)                                                                                                                aaTAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATACAAGCGAATCAAAAAATATCGGGGGGAAg                   >  1:429379/1‑143 (MQ=255)                                                                                                                aaTAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCAACAGAAAGAAAAACGCCAGTGCTAAAAGCAATTCAAAAAAAATCGATGGGAAg                   >  2:14322/1‑143 (MQ=255)                                                                                                                      aCAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGc             >  1:369305/1‑143 (MQ=255)                                                                                                                         tggcgcgatatAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCa          <  2:808004/136‑1 (MQ=255)                                                                                                                           cagcaACAGGGCGCGGTGCTGGACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCaaa        <  1:202184/143‑1 (MQ=255)                                                                                                                            agcaATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGAACACGGCGCACCAGCCAGAAAATCGCCAGAGATGAAAGCAAATCAAAAAATCTCGCTGGAAAGGACAGCCCacac       >  2:343267/1‑143 (MQ=255)                                                                                                                                aTAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCCAAAAATATCGGTGGGAAGGACAGCCCAAAcacca   >  2:932597/1‑143 (MQ=255)                                                                                                                                 tAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAAcaccac  <  1:875693/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                   ATTAAAAATTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCCAAACACCAC  >  NC_000913/3389046‑3389316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑