Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 756,792 G→T E296* (GAA→TAA)  sdhA → succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,7920GT95.8% 130.5 / NA 48E296* (GAA→TAA) sdhAsuccinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (25/21);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAT  >  NC_000913/756652‑756929
                                                                                                                                            |                                                                                                                                         
cGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCCGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTaa                                                                                                                                         <  1:696440/143‑1 (MQ=255)
 gggCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGTCCGTGACGTGGTCGCGCGTTCCACTATGATCTaaa                                                                                                                                        >  1:41984/1‑143 (MQ=255)
 gggCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTaaa                                                                                                                                        >  1:666090/1‑143 (MQ=255)
          ggTCACCGAAGGGGGCCGCGGTGCAGGCGGTTATCTGCTGAACAGCCGTGTCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAg                                                                                                                               <  1:36619/143‑1 (MQ=255)
           gTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAgg                                                                                                                              >  1:693618/1‑143 (MQ=255)
            tCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGAGTTCCATCATGGTCTAAATCAGAGAATGt                                                                                                                             >  1:814832/1‑143 (MQ=255)
               ccGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTcgc                                                                                                                          <  2:922144/143‑1 (MQ=255)
                ggggggTTGGCGTGGTGGAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTAGGGGGCGTTGTGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTcgcg                                                                                                                         <  2:436650/139‑1 (MQ=255)
                cGAAGGTTGACGTGGTGAAGGCGGTTATATGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACATGGCGGGCCGTGACGTGGTTGCGCGTTCCATCCTGATGTAAAGCCGAGAAggcccca                                                                                                                         >  2:6600/1‑138 (MQ=255)
                    ggTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCtg                                                                                                                     >  2:221233/1‑143 (MQ=255)
                          cGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATgg                                                                                                               >  1:408130/1‑143 (MQ=255)
                             ggtgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCGATCATGATCTAAATCCGTGAAGGGCGCGGCTGTGATGGTcc                                                                                                            >  1:406229/1‑143 (MQ=255)
                                 aaGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTgg                                                                                                        >  1:918801/1‑143 (MQ=255)
                                    gCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTACATCCGTGGAGGTCGCGGCCGCGAGGCTGCGTggggg                                                                                                     >  1:912230/1‑143 (MQ=255)
                                          ctccgcggAACAAACACTGCGATCGTTGTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCacac                                                                                               <  1:216188/136‑1 (MQ=255)
                                               ctgAACAAACGTGGCGTACGTTTTATGGTGCGTTATGCGCCGATCGCCAAATACCTGGCGGGCCGTGTCGTGGTTGCGCGTTCCATCATGATCTAAAGCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaa                                                                                          <  1:572576/143‑1 (MQ=255)
                                               ctgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGCTGGTCCGTGGGGGCCACAcgcgca                                                                                          >  2:758362/1‑141 (MQ=255)
                                                 gAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaac                                                                                        >  1:643157/1‑143 (MQ=255)
                                                       acaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTACATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGCTGGTCCGTTGGGGCCACACGCgaaaccgcaac                                                                                  >  2:866308/1‑143 (MQ=255)
                                                          tGGCGAACGTGTTATGGAGCGTGGTGCTCCGAACGCCAATGAGCCTGCTTGGTGTGTCGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCg                                                                               <  1:132261/143‑1 (MQ=255)
                                                                  gttgtgtGGAGCGTGATGCGCCGAACGCCAACGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTg                                                                       <  1:19986/137‑1 (MQ=255)
                                                                    tttATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTggg                                                                     >  2:710866/1‑143 (MQ=255)
                                                                         gaggCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGCTAaag                                                                <  2:813616/140‑1 (MQ=255)
                                                                           aGCGTTATGCGCCGAACGCCAAAGACGTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaa                                                              <  1:981255/143‑1 (MQ=255)
                                                                           aGCGTTAGGGGCCGCACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGAGCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaa                                                              <  2:849639/143‑1 (MQ=255)
                                                                                tATGCGCCTAACGCCAAACTCCTTCCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAATGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtct                                                         <  2:79526/143‑1 (MQ=255)
                                                                                 aTGCGCGGATCGCCAAAGACCTGGCGGGTCGTGACGTGGTTGCGCGTTCCATCTTGATCTAAATCCGTGAAGGTCGCGGCTGAAATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtctc                                                        <  1:366501/143‑1 (MQ=255)
                                                                                   gcgcCGAACGCCAAAGACCTGGCGGGCCGTGCCGTGGTTGCTCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGa                                                      <  1:405765/143‑1 (MQ=255)
                                                                                    cgcCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGCAACTGAAACTCGATCACCTGGGTAAAGCAGTtctccca                                                     >  2:439034/1‑140 (MQ=255)
                                                                                     gcCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGCTCACCTGGGTCCAGAAGATGTCGAAt                                                    >  1:128121/1‑143 (MQ=255)
                                                                                         aaCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATAGCCTGGGGGAAGAAGGTCTCGAATCCCg                                                >  2:382269/1‑143 (MQ=255)
                                                                                             ccACAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAAGCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTg                                            <  1:761122/143‑1 (MQ=255)
                                                                                              cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACAAGCGAAACTGAAACTCGATCACCTGGGAAAACAAGCTGTCGAATCCCGTCTga                                           >  2:287212/1‑142 (MQ=255)
                                                                                                 aGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCgg                                        <  1:710866/143‑1 (MQ=255)
                                                                                                   aCCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGCAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCCCCTGGGTAAAGAAGTTCTCGAATCCCATCTGCCGGGt                                      >  2:122583/1‑143 (MQ=255)
                                                                                                   aCCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGACTCCCGTCTGCCGGGt                                      >  2:185505/1‑143 (MQ=255)
                                                                                                   aCCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGt                                      <  1:803441/143‑1 (MQ=255)
                                                                                                    ccTGGCGGGCCGTGACGTGGTTGCGCGTTTCATCATGATCTAAATTCTTGTGAAAGACGGCGGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTa                                     <  1:221233/143‑1 (MQ=255)
                                                                                                     cTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCCCACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTAt                                    >  1:491664/1‑143 (MQ=255)
                                                                                                          tggCCGCGACGTGGTTGCGCGTTCCATCATGATCTACATTCGTGGAGGGCGCGGCTGTGCTGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTgg                               <  1:764276/142‑1 (MQ=255)
                                                                                                           ggCGGTGGCGGGGTGGCGGGGTCCAGCATGATCGAGATCCGGGAAGGTCGCGGCTGGGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGa                              <  2:129961/143‑1 (MQ=255)
                                                                                                              cGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGCAGCt                           >  1:280699/1‑143 (MQ=255)
                                                                                                                       gTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTGCTCGAATCCCGTCTGCCGGGTAGCCTGGAGCGTTCCCGTag                  >  2:460208/1‑142 (MQ=255)
                                                                                                                         tGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACCCGCGAACCTGAAACTCGATCACCTGGGTAAAGACGTTCTCGCATCCCGTCTGCCGGTGCTCCTGGAGCTTTCCCGTAccc                >  1:174563/1‑142 (MQ=255)
                                                                                                                            gcgTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGATATACTGGAGCTTTACCGTACCTTCg             >  1:633164/1‑143 (MQ=255)
                                                                                                                                 ccATCATGAGCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCAc        <  2:918801/143‑1 (MQ=255)
                                                                                                                                    tcatGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGACGTTCTCGAATCCCGTCTGCCGGGTATCCTGGACCTGTCCCGCACCCTCGCtgtcgta     >  2:93414/1‑137 (MQ=255)
                                                                                                                                       tGATCTAAACCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt  <  1:315407/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
CGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAT  >  NC_000913/756652‑756929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑