| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,466,201 | T→C | 14.2% | pseudogene (810/2518 nt) | ydbA → | putative outer membrane protein, N‑terminal fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,466,201 | 0 | T | C | 14.2% | 106.3 / 6.2 | 50 | pseudogene (810/2518 nt) | ydbA | putative outer membrane protein, N‑terminal fragment |
| Reads supporting (aligned to +/- strand): ref base T (13/27); new base C (6/2); total (19/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
CCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACC > NC_000913/1466062‑1466336 | ccACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGAc < 1:499884/143‑1 (MQ=255) caGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgc > 1:501309/1‑143 (MQ=255) aGTAAACAACAATGGCAAAACCACCGTTGTCTGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAgc‑g < 1:476539/143‑1 (MQ=255) caacaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGC‑GCAACgg > 2:194165/1‑143 (MQ=255) caacaaTGAGAAAACCACCGTGGACCGTAAAGATTCCACCGTTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGCACGTCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACgg < 1:34885/143‑1 (MQ=17) agggCAAAACCACCGTTGCCGGTAACGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGAt < 1:8782/141‑1 (MQ=255) taatcaccGTTGACGGTAAGGGTTCCACCGGTACGCAAATCAACGGGAATAACGGGAGAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGTGGTGACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAg < 2:559734/139‑1 (MQ=14) aaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGC‑GCAACGGt > 1:238579/1‑133 (MQ=255) aaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGC‑GCAACGGt < 2:238579/133‑1 (MQ=16) aaCCACCGTTGACGGTAAAGATGCCACCGGTACGGAAACCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAgg < 2:347307/143‑1 (MQ=255) ccaccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGCTATTACCGGTGACAGC‑GCGACGGTGGATAACAAGGGc < 1:147121/143‑1 (MQ=255) caccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCa < 2:563710/143‑1 (MQ=255) caccGTTGACGGTAAAGATTCCACCGGCACGGAAATCAACGGTAATAACGGGAAAGTGATGCAGGACGGCGAACTGGAAGTCAGCGGCGGCGGCCACGGAGTTGATACCACCGGTGACAGC‑GCGACGGCGGAGAACAAGGGCa > 2:159366/1‑143 (MQ=14) ccGTAGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCAcc < 1:300872/143‑1 (MQ=255) gACGGGAAAGATTCCAGCGGTACGGAAGTCAACGGTAACAACGGGAACGTGCTTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATACACGGACACAAC‑TCGATAGTGGATAACAATGGCACCATGAc < 2:446304/143‑1 (MQ=14) tAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCa < 1:45945/143‑1 (MQ=255) aaaGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGGCGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCAc < 1:616837/143‑1 (MQ=255) gATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGa > 2:76397/1‑101 (MQ=17) tCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGGAGCATGACCG‑TCACCGATcc > 1:173763/1‑143 (MQ=255) ccACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCg > 1:374872/1‑143 (MQ=40) gTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAg > 1:456645/1‑94 (MQ=25) gTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAg < 2:456645/94‑1 (MQ=17) aaaTCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGt > 1:299958/1‑143 (MQ=255) tCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATc < 2:501309/143‑1 (MQ=255) aCGGTAATAACGGGAAAGTGATTCAGGGAAACGTGCCGGAAGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGC‑GCGACGGTGGATAACAAGGGCACCAAGCCCG‑TCACCGATCCGGCGTCCATGGGTGACCAg > 2:45585/1‑143 (MQ=14) cGGGAATAACGGGAAAGTGATGCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGa < 1:19686/143‑1 (MQ=255) gttaataaCGGGAAAGTGATGCAGGTCGGCGATCTGGACGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGAt < 2:328570/141‑1 (MQ=255) ggTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGAt < 2:278407/143‑1 (MQ=255) ggTAATAACGGGAAAGTGATTCAGGACGGCGATCTCGAGGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGAt < 1:271001/143‑1 (MQ=255) taaCGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGC‑GCGACGGTg > 1:190328/1‑85 (MQ=18) aCGGGAAACGGCTTCCGGACGGCTAGCGGGATGTCAGCGGCGGCGGTCACGGTATTGATAGCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGt < 1:319234/143‑1 (MQ=14) aaaGTGATTCACGACTGCGATCTGGTTGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACaa < 2:88190/143‑1 (MQ=255) gTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGCTCGACGGTGACAAGGc > 1:43368/1‑143 (MQ=255) aGGACGGCTATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATACCAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTc < 2:599277/143‑1 (MQ=255) cGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACg > 1:406485/1‑143 (MQ=255) gATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGa < 2:117000/143‑1 (MQ=255) aTCTGGATGTCAGCGGCGGCAGTCACGGTACTGATAGCACCGGGGACAGC‑GCGACGGTGGATAACAAGGGCACCATGCGCGATCA‑CTATCCTTTGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGaa < 1:260914/143‑1 (MQ=14) gATGTCGGCGGCGGGGGTCACGGTATTGATATCACCGGTGGCAGCAGTGAACG‑GGATGACTGGGGGGGCAGGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCga < 2:130994/143‑1 (MQ=14) gATGTCAGCGGCGGCGGTCACGGTATTGATATCTCCGGTGACAGC‑GCGACGGTGGATATCAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCga < 1:298288/143‑1 (MQ=255) gATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCga < 2:583472/143‑1 (MQ=255) tGTCAGCGGCGGCGGTCACGGTAATGATATCACCGGTGACAGC‑GCGACGGTAGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCgaga < 1:331153/143‑1 (MQ=255) gcggcggcgGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGCGCAcc > 1:59070/1‑143 (MQ=255) cggcggTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGTTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCa < 1:277402/143‑1 (MQ=255) ggTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGAAAAGGCAATCGTCAATAACGAAGGCGAGAGCCCCATCACCaa > 2:445142/1‑143 (MQ=255) tCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACg > 1:94585/1‑143 (MQ=255) tCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACg < 1:604099/143‑1 (MQ=255) ttGAGATGACCGGTGGCAGC‑GGGACGGTGGAGAACAAGGGCTCCATGAGCG‑TCACGGATGCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCCCCAACGGttttacc < 2:252630/143‑7 (MQ=17) tGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTc > 2:533512/1‑66 (MQ=40) gATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCCATAACGAAGGCGAGAGCACCCTCACCAACGGTggcaccgg > 1:421618/1‑143 (MQ=255) tCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGGTCGACGGCGACAAGGCCGTCGTCAATAACGAAGGCGAGAGCCCCATCAGCATCGGTgccaccggcacg > 2:213349/1‑142 (MQ=14) | CCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGC‑GCGACGGTGGATAACAAGGGCACCATGACCG‑TCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACC > NC_000913/1466062‑1466336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |