| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 98.9% | 76.0 / ‑6.7 | 27 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (13/13); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG > NC_000913/2132645‑2132924 | gCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTAGAATTTGTCTTTGTGCTTGCGGTACAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑CGAGTGACATGCGGGa > 2:129710/1‑143 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGc < 1:391937/143‑1 (MQ=255) aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAc > 1:338050/1‑143 (MQ=255) cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTGCTTTcc > 1:289569/1‑143 (MQ=255) atTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTGCCCGGAGAATTTTTTCGGTGACGGGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGt < 2:315359/143‑1 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGtt > 1:309727/1‑143 (MQ=255) tGCTGGCGCGGTCGAATGTGTCTGTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg < 2:201106/143‑1 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGATAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc < 1:28028/143‑1 (MQ=255) cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGTCGCTGCGTCGCCTCTTCTACTTCCTACGGTTAGCCGTACTcc > 2:572249/1‑143 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGATCTGCATTTCACCG‑TGATTGTTATGCAGGCGCGGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacca > 2:70803/1‑143 (MQ=255) aaTTTGTCTTTGTGCTTGCGGTATAAGTTTGAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCCCCAt > 2:546440/1‑143 (MQ=255) aaTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTACCCGGTTCGCGGTACTCCAGCACCAt > 2:342066/1‑143 (MQ=255) tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGCGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATcc < 1:90222/143‑1 (MQ=255) ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa > 1:307503/1‑143 (MQ=255) tgcGGAGAATTTTTTCGAGGACGAGGCGACCAGCAGGTCACCG‑CGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 1:228048/141‑1 (MQ=255) actgagaATTTTTTCGTCTACGAGTTCATCTGCATTTCACCT‑TTATTGATATGCAGGCTCTGCTTCGCTTCTTCTCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg < 2:81080/140‑1 (MQ=255) gcagaATTTTTTCGGTGACGGGGAGATCGCCATGTCACCG‑AGGTCAGTATCCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt < 1:84460/141‑1 (MQ=255) atctgttCGGTGACGAGGTGAGCTGCCTTTCCCCG‑GGATTGCGAGCCCGGCCCGGCGCCGCCTCCTCCACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTa < 1:23103/138‑1 (MQ=255) ttttttCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTACACttagatct > 2:284769/1‑137 (MQ=255) ttgttgggTGACGAGGTGATCTGCCTTTCCCCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc < 2:309727/137‑1 (MQ=255) ttCGGTGACGAGGTGCTCTGC‑TTCGATTGTTGGTGGATATGGAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 1:84857/143‑1 (MQ=255) tGATCTCCCTTTCACCG‑TGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTg < 2:561051/143‑1 (MQ=255) gCATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCCGGAAGATGTCGTAATCCTGTGCCCCGTTCGGTTCGCTCTCGAACAAACACTCATAGAAAccc > 1:35014/1‑141 (MQ=255) cATTTCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCACGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc > 1:312201/1‑143 (MQ=255) ttCACCG‑TGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCAACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATg > 2:23958/1‑143 (MQ=255) gattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTGATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCCTCTTTGAAACGCCATGCCCAggcc > 2:199442/1‑141 (MQ=255) ttgatGTGCAGGCTTTGTGTCGCCTCTTCTACTGTCCAGGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa < 2:253620/143‑1 (MQ=255) gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg < 1:104811/143‑1 (MQ=255) | GCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCG‑TGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG > NC_000913/2132645‑2132924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |