| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 192,963 | G→A | 15.0% | R31H (CGT→CAT) | frr → | ribosome‑recycling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 192,963 | 0 | G | A | 15.0% | 60.0 / 3.3 | 32 | R31H (CGT→CAT) | frr | ribosome‑recycling factor |
| Reads supporting (aligned to +/- strand): ref base G (13/14); new base A (3/2); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01 | |||||||||||
GTGTGTGACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCT > NC_000913/192823‑193096 | gtgtgtgtgtgtGTGCTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCGGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTg < 2:567358/143‑1 (MQ=255)gtgtgtgACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGCGTCGTg > 1:94945/1‑143 (MQ=255) gtgACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCGTGGACAAATGCGTAGAAGCGTTCAGAACCCAAATCAGCAAAATACGCACGGGTCGTCCtta > 2:478280/1‑142 (MQ=255) gACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTCACGTGATTCGCGATATCAGAAGCGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCCAATCCGCCAAATACCCACGGGCCGTGCCtccc > 2:247342/1‑141 (MQ=255) tGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCATGCTTCTCCCa < 2:218552/143‑1 (MQ=255) tctggtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGACGCGTTCAAAACCCAAATCAGCAAACTACGCACGGGGCGTGATTTGCCCAGc > 2:66497/1‑143 (MQ=255) ggtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCctg < 1:225528/143‑1 (MQ=255) aCAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAACGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCAtt > 1:184372/1‑143 (MQ=255) aaGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCATGCTTCTCCCAGCCTGCTGGATGGCATTGt > 1:43670/1‑143 (MQ=255) tCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGACTGCTGGATGGCATTGTCGAGGa > 1:523696/1‑143 (MQ=255) gATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATAtt > 1:64558/1‑143 (MQ=255) aTTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGAGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGCCATGCTTCTCCCAGCCTGCTGGATGGCACTCTCGTGGAATAttg > 2:560146/1‑142 (MQ=255) aTTCGTAACGGGAGTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTa < 1:302671/143‑1 (MQ=255) ttAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCacgccgacg < 1:375240/143‑1 (MQ=255) atatCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATCGTCGTGGAATATTCCGGCCCGCCGACGCCGCtc > 2:361470/1‑142 (MQ=255) tatCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTGAAAAACCCAAATCAGCAAAATACGGAAGGGTCGTGCTTCTCCGAGCCTGCTGGATGGCATTGTGGTGGAATATTACGGCACGCCGAGGCCGCTGc > 1:297474/1‑143 (MQ=255) aGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATGACGGCACGCCGACGCCGCTGCGTCa > 1:220358/1‑143 (MQ=255) aaaaTATGGTCAAGTACTCATGGACAAATGCGTATAAGCGTTCCAAACCCAAATCAGCAAAATACGCACGGTTCGTTCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTAAAGCACGCCGACTCCGCTGCGTCAGc < 2:379845/143‑1 (MQ=255) aGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTgg < 1:501191/143‑1 (MQ=255) gaggAAGTACGCATGGACAAATGCGTAGAAGCGTTGAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAg < 1:238988/140‑1 (MQ=255) gAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGt > 1:180130/1‑143 (MQ=255) actaggCATGGACAAATGCGTGTAAGCGTTCAAAACTCACCTCAGCAAAATCCGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTaa < 2:174277/138‑1 (MQ=255) tGGACAATTGCGTAGAAGCGTTCAAGACCCAAGTCAGCAAAATCCGCACGGGTCGTGCTTCCCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGTCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTagaa < 1:158757/143‑1 (MQ=255) tGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGTTGCCATTGTCGTGGAATAGTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTagaa < 1:57257/143‑1 (MQ=255) gtgACAAAGTCGTAGCAGCCTTCAAACCCCAAAACAGCTAAACACGCACGGGTCGTGCTTCTCCCAGCCGGCTGGAAGGAATCGTCGCCGAATATTACGTCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTagaa < 1:56854/141‑1 (MQ=255) cAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAACATTCCCGTACACTGGAAATCa > 1:63359/1‑143 (MQ=255) ataaCCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCaa < 2:64558/141‑1 (MQ=255) aaaCCCAGAGCAGCAAAACACGCACGGGTCATGCTTCGCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAAc < 1:82356/143‑1 (MQ=255) ccAAATCAGCAAAATACGCACGGGGCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACgtgt < 2:403265/143‑1 (MQ=255) cAAAATACGCACGGGTCATGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGCTTGATCGtt > 1:471534/1‑143 (MQ=255) aaTACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCaa > 1:306796/1‑143 (MQ=255) gCACGGGGCGTGCTTCTCCCACCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGtct < 1:185679/143‑1 (MQ=255) | GTGTGTGACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATTGTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCT > NC_000913/192823‑193096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |