| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,398 | T→G | 100% | L29R (CTT→CGT) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,398 | 0 | T | G | 100.0% | 106.0 / ‑6.7 | 36 | L29R (CTT→CGT) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (21/14); minor base C (1/0); total (22/14) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTG > NC_000913/3041269‑3041537 | tccATTAGTCAGTTATACCCCCCTACGCAACTCAGGACTCTATTATGTCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACt < 2:295727/142‑1 (MQ=255) tAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCCCCCTTACTGgcgc > 1:35622/1‑143 (MQ=255) aGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGcgcg < 1:465423/143‑1 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa < 1:209775/143‑1 (MQ=255) aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACAATAGAGGGGTGGGCGCGTGCCACCATTACTGGCGCGGACa > 1:421926/1‑143 (MQ=255) cGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCGCCATGACTGGCGCGGACAGCGaaaa > 1:182791/1‑143 (MQ=255) gCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCa < 2:454259/143‑1 (MQ=255) cAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTATGCCCGCGTATCGCTGTCGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAg > 1:558873/1‑143 (MQ=255) aaCTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAgg < 1:122414/143‑1 (MQ=255) aGGACTCTTTTATGGCTGTTACGCCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCGGAGGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg < 2:156716/143‑1 (MQ=255) aGGACTCTATTATGGCTTTTACGCCTATTACCACCCATTACCCTACGGCTTCTGCCCGTTTACCGCCGCCTCGCATGGCGCTTGTGGACATTTCTCCTCCCACCATTACGGGCCAGGACAGCGGGAAATATATACAGCGTCAg > 2:304535/1‑143 (MQ=255) gACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCAGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCAACATTACTGGCGCGGACAGCGACAAATATATGCAGGGGCAggg > 2:334800/1‑142 (MQ=255) ctgtcttagGGCGTTTACACCTGTCCCCCCCCGTCAGCACCCGGCTTCTGCGCGTCCAACGCAGACGCCGAAGACGCTTGATGACTGGGGGCGTGCCAAAATCACTGACGCCGACAGCGAAAAATATATGCAGGGTCAGGTGa < 2:412516/138‑1 (MQ=255) ctctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGa < 1:316503/143‑1 (MQ=255) ctccATTATGCCTTTTTCACCTCGTCCTCCCCGTCGGCCTACGGCGTCTGCCCGTCTACCGCGGACGTTGATGGCGCGTGAGGACTGGGCGCGTGCCTCCGTTGCTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGa < 2:340927/143‑1 (MQ=255) tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAGCGGAAAATATATGCAGGGTCAGGTGAcgg > 1:403698/1‑141 (MQ=255) tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCCTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAccg > 2:456580/1‑141 (MQ=255) ttatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGGCTGGGCGCGTGCCACCATTACTGGCGCGGACCGCGAAAGATACATGCAGGGCCAGGTGAcagcc > 2:302006/1‑142 (MQ=255) gCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACAATTACTGGCGCGGACAGCGAAAAGTATATGAAGGGTCAGGAGACAGCAGATgg > 1:51408/1‑142 (MQ=255) ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa < 2:104628/143‑1 (MQ=255) gtttACACCTTTGCCTCCCCGTCAGCCTACGGCTTCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa < 2:8067/142‑1 (MQ=255) acCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCGGGGTCAGGTGACAGCAGATGTCAGCCAgg > 1:333607/1‑142 (MQ=255) ttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAGATATATGCAGGGTCAGGTGAAAGCAGGTGTCAGCCAGATGGCa > 2:526315/1‑143 (MQ=255) ctccCCGTCAGCCTACGGCTGCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa < 2:14919/143‑1 (MQ=255) ccttCAGCCTACTGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc < 2:53663/140‑1 (MQ=255) tCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGc > 1:55216/1‑143 (MQ=255) ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCt > 1:469785/1‑143 (MQ=255) ttCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccg > 1:101868/1‑143 (MQ=255) cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGACACATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAGCAGCACCTGCTCGCCGCCCAt > 2:342379/1‑143 (MQ=255) tttACCGCTGACGATGATGACGCTTGATGTCTGGGCGCGTGCCATCATTACTGGCGCGGATAGCGAAACATATATGCGGGGTCAGGCGACAGCCGCTGTCAGCCAGATTGCAGAAGATCAGCACCTGCTCCCCGCCCATTCCg > 2:119630/1‑143 (MQ=255) tACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCgcc > 1:398204/1‑141 (MQ=255) aCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCGGGGTCAGGTGACAGCAGATGGCAGCCAGATGGCAGGCGAGCAGCACCTGCTCGCCGCCCAGTacgacg > 2:244523/1‑143 (MQ=255) aCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGAACAGCAAAATATATGTGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCCCCTGCTCGCCGCCCATTGCGACg > 1:559035/1‑143 (MQ=255) gaCGCTTGATGACTGGACGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTACAAtgt > 2:619519/1‑143 (MQ=255) tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGACGCCCATGGCGACGCCAAAGGTAAAATGTGGAGCAATTGACg > 2:372516/1‑143 (MQ=255) gggCGCGTGCCACCATGACTGGCGCGTACACCGAAAAATATATGCAGCGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCATCACCTGCGCTCCGCCCATTGCGACGCCAAAGGTAAAATTTGGAGCAATTTACGt < 1:304535/143‑1 (MQ=255) cgcgTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCGGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACggccg > 2:69712/1‑139 (MQ=255) | ACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTG > NC_000913/3041269‑3041537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |