| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,389,186 | T→C | 100% | N51S (AAC→AGC) | aaeX ← | DUF1656 domain‑containing protein AaeX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,389,186 | 0 | T | C | 100.0% | 70.6 / NA | 26 | N51S (AAC→AGC) | aaeX | DUF1656 domain‑containing protein AaeX |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/14); total (12/14) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTAAAAATTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCC > NC_000913/3389047‑3389307 | ttAAAAATTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGa > 1:492801/1‑143 (MQ=255) tAAAAATTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAACCAAGCAGCAATAGAGCGCGGTGCTGaa > 1:188141/1‑143 (MQ=255) tAAAAATCGCGATGAAGGCCAGAATGACTAGTACGACCGTGATGGCCGTACGGGAGAATTTTCTTACGAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGaa < 2:64310/143‑1 (MQ=255) tAAAAAGTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGaa < 2:367439/143‑1 (MQ=255) aaaTTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGATTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGaacaa > 2:141497/1‑143 (MQ=255) ataaaGGCCAGAACGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTACAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGAt < 2:259050/140‑1 (MQ=255) aTGAAGGGCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGAt < 2:258265/143‑1 (MQ=255) aaGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAACACCAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGcc > 1:229178/1‑143 (MQ=255) gggcgtgAAGGACCAAGACGAGGGTGGTGGACGTACGGGAGAATTTGCTGATTAGTGTTTTCACGTCAAGCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCa < 2:196373/137‑1 (MQ=255) cAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGACCGACa > 1:127454/1‑143 (MQ=255) aaTGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAg > 1:604836/1‑143 (MQ=255) cTATTACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTa < 1:549241/143‑1 (MQ=255) cTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAGCGAGCAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCTGACAAAGTCGTa < 1:348294/143‑1 (MQ=255) cTAATACGACCGTGATGGCCGGACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACACGAAGCAATTTAGCGCTGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTa < 1:593435/143‑1 (MQ=255) tACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAAAAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGGCCTGGATa > 2:448363/1‑143 (MQ=255) cGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATGAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATAcc > 1:326114/1‑143 (MQ=255) gggCCGTACGGGAGAATGTTCGTATTAGTGTTTTCACTTCCACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGcac < 1:197895/142‑1 (MQ=255) gTACGGGAGTATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTa < 1:568709/143‑1 (MQ=255) tttCTTATTAGTGTTTTCACATTAACCACAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCACTAGAGCGTGGTGCTGAACAACGACGGCTGCCAGAAAAAGTCGTAGATACCGAGTGGCACAAGTACCCGGCGCAcaa > 2:161088/1‑141 (MQ=255) aGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGaaaa > 1:592537/1‑143 (MQ=255) tgtTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATc < 1:620000/143‑1 (MQ=255) cgagctCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATc < 1:155945/138‑1 (MQ=255) atCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTTGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTgg < 1:1201/143‑1 (MQ=255) tCAAATAAAACAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTggg > 1:563832/1‑143 (MQ=255) aaCAAGCAGCAATAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAg > 1:27229/1‑143 (MQ=255) ccgccgcaACAGAGCGCGGTGCTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGccc < 1:102834/138‑1 (MQ=255) | TTAAAAATTGCGATGAAGGCCAGAATGACTAATACGACCGTGATGGCCGTACGGGAGAATTTTCTTATTAGTGTTTTCACTTCAACCTCAAACGAACAGTCGCGATATCAAATAAAACAAGCAGCAATAGAGCGCGGTGTTGAACAACGCCGGATGCCAGACAAAGTCGTAGATACCTGTTGGCACAAGTACCCGGCGCACCAGCCAGAAAATCGCCAGTGATAAAAGCAATTCAAAAAATATCGGTGGGAAGGACAGCCC > NC_000913/3389047‑3389307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |