| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,954,273 | G→T | 8.4% | E266* (GAA→TAA) ‡ | ilvD → | dihydroxy‑acid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,954,273 | 0 | G | T | 8.4% | 139.2 / 3.5 | 58 | E266* (GAA→TAA) ‡ | ilvD | dihydroxy‑acid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (23/29); new base T (3/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01 | |||||||||||
GCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATAAGCTTTCCCGCAAGGTTCCACA > NC_000913/3954133‑3954406 | gCTCGCTGCTGGCACCCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGaa < 2:568647/143‑1 (MQ=255)gCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGaa < 1:219848/143‑1 (MQ=255) ctgctgGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGc < 1:360651/143‑1 (MQ=255) tgctgGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTTCAAACGcc < 1:607505/143‑1 (MQ=255) cttgCAACTTACGCCGACCGTAACTAGCTGTTCCCTAATGGTGGTAAACGCATTGTCGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTTCAAACGccgc < 2:174714/140‑3 (MQ=255) ctgGCAAGCCACGACGACCGTAAGCAGCTGTTCCTTAATGCTGGGAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCAt < 1:563656/143‑1 (MQ=255) ctgGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTGAGGCGGCGTTTGAAAACGCCag > 2:613529/1‑142 (MQ=255) tgGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAGCGTTATTACGAGCAAAACGACGAAAGTGCACTGACGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCagg > 1:372090/1‑141 (MQ=255) ggCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTTAAAACGCCATGa > 1:356680/1‑143 (MQ=255) ggCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGa < 1:107308/143‑1 (MQ=255) aCCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGGATGGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGTGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCt < 1:54865/143‑1 (MQ=255) cGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTCAAAACGCCATGACGCTGCata > 2:190263/1‑143 (MQ=255) gACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGCTATTACGAGCAAAACGACGAAAGTGCAGTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATcgc < 1:357245/143‑1 (MQ=255) ccGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGa < 1:366596/143‑1 (MQ=255) cGTAAGCAGCTGTTCATTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAACCGCCATGACGCTGGATATCGCGAt > 2:526386/1‑143 (MQ=255) tAAGGAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATgg < 2:348436/143‑1 (MQ=255) tAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATgg < 2:296295/143‑1 (MQ=255) cTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTCAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTaa > 1:327964/1‑143 (MQ=255) ttAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGAGGAACGACTaaa > 1:324897/1‑142 (MQ=255) ttAATGCTGGTAAACGCATTGTAGAAATGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAac > 2:546615/1‑143 (MQ=255) gtGCTGGTAAACGCATTGTTTAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGTCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACAcc < 2:589923/142‑1 (MQ=255) cTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTa < 1:344023/143‑1 (MQ=255) tGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTAc > 1:306013/1‑143 (MQ=255) ggTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACt > 1:332372/1‑143 (MQ=255) gTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACtt > 1:140039/1‑143 (MQ=255) tAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCCAGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTCCTTc > 1:139769/1‑143 (MQ=255) caaaCGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTc < 1:330642/142‑1 (MQ=255) ttgttgAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACctgctg > 1:110084/1‑143 (MQ=255) ttgAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTggcc > 2:197675/1‑142 (MQ=255) gAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTggcggc > 1:569302/1‑143 (MQ=255) gACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGgcgc < 1:360671/143‑1 (MQ=255) aCCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCa > 1:386739/1‑143 (MQ=255) aCCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGAGTAAAAG‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGCTCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCa > 1:171323/1‑143 (MQ=255) ccgaaCGTTTTTACGAGTAAAACGATGAAAGTGCACTGTCGCGTAATAT‑CGCCTGTAAGGCGGCGGTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAg < 1:396206/140‑1 (MQ=255) cttataCGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCgg < 2:162095/139‑1 (MQ=255) caGCAAAACGACGAAGGGGCACTGCCGCGTAATAC‑CGCCAGGAAGGCGGCGTTGGAAGACGCCATGACGGTGGCTATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGa < 1:80007/142‑1 (MQ=255) gCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAAAACCGTACTTCGCCTGCTGGCGGCGGGGCAGGAGGAGGAAATCGACt > 1:48362/1‑143 (MQ=255) aaaCGACGAAAGTGCACTGCCGCGTGATAT‑CGCCAGTAAGGCGGCGGTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCa > 2:218201/1‑143 (MQ=255) acgaAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATg > 1:568579/1‑143 (MQ=255) cgaAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGa < 2:388792/143‑1 (MQ=255) cgaAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGa < 2:246313/143‑1 (MQ=255) aaaggacACTGCCGCGTGATAG‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGt < 1:528750/137‑1 (MQ=255) aGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCAAGAGTgg > 1:110150/1‑142 (MQ=255) gCACTGCCGCGTAATCTACACCAG‑ATGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTCACACCGTACTTCACCTGCTGGCGGCGGCGCAGGCAGCGGAAAGCGACTTCAGCATGAGAGatag > 2:379532/1‑142 (MQ=255) aCTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGAGGCTGGATATCGCGATGGGTGGCTCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCCGGAAGCGGAAAGCGACGTCCCCCTGCGTGTTATCg > 1:434606/1‑143 (MQ=255) aCTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCg > 1:197348/1‑143 (MQ=255) tGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGAt < 2:110084/143‑1 (MQ=255) tGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGAt > 1:473505/1‑143 (MQ=255) gtcctcgTACTAT‑CGCGCGTAAGGCTGCGGTTGAAGACGCCATGAGGCTGGACAGCGCGATGGGTGGATCGACTAACACCGTATTTCACCTCCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGAt < 2:241046/138‑1 (MQ=255) ccGCGTAATAT‑CGCCAGTAGGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATaa < 2:491815/143‑1 (MQ=255) ccGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATaa < 1:403112/143‑1 (MQ=255) gcgTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATAAGc < 1:565586/143‑1 (MQ=255) ccAGCAAGGCGGCGTTGGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATAAGCTTTCCCGCAAg < 2:233847/143‑1 (MQ=255) gcgtcatggcgTTTTCAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATAAGCTTTCCCGCAAGGtt > 2:292429/8‑143 (MQ=255) gTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATAAGCTTTCCCGCAAGGtt < 1:515487/143‑1 (MQ=255) aaGGCGGCGTTTGATAGCGCCAGGACGCAGGATATCGCGATGCGTGGATTCACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATAAGCTTTCCCGCAAGGTTcc < 2:69300/143‑1 (MQ=255) aaGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATAAGCTTTCCCGCAAGGTTcc < 1:307799/143‑1 (MQ=255) atggcgTTTTCAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCCCCATGCGAGATATCGATAAGCTCTCCCCCACGCTTCcaca > 2:370787/3‑143 (MQ=255) | GCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATAT‑CGCCAGTAAGGCGGCGTTTGAAAACGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAATCGACTTCACCATGAGTGATATCGATAAGCTTTCCCGCAAGGTTCCACA > NC_000913/3954133‑3954406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |