Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,247	A→G	100%	L375M (TTA→CTG)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,247	0	A	G	100.0%	30.4 / NA	14	L375M (TTA→CTG)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/5);  total (9/5)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACA‑TGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTT  >  NC_000913/1196113‑1196300                                                                                                                                        |                                                      gAATGCGCCCGGTTTGAAGCCACCCACAGTACTGCGCCGAAATATGCCGGTCAGGACAAAGGAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTaa                                               <  1:124295/143‑1 (MQ=37)   aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                             <  2:33469/143‑1 (MQ=255)          cTGTTTGAAGCCATCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa                                      <  2:574646/143‑1 (MQ=37)           tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAACCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGGCCTGATTGGTAAAGGTATGGaa                                     >  1:596189/1‑143 (MQ=37)            gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAg                                    >  1:500875/1‑143 (MQ=255)              gtGAAGCCTCGCGCGGTACTGCGTCGGAAGATGCGTGTGAGGACAATGTCAATCCTGGCTGTATTGTTCGCTCGGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgc                                  <  2:407995/142‑1 (MQ=12)                        ccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg                        >  1:277124/1‑141 (MQ=25)                        ccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACCGAAGCGGCTGACCTGAGTCTTACAGGTACGGCAGGCgcaagcaatg                        >  2:531422/1‑141 (MQ=25)                                   cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGAGGAGGAGGCG‑CACACCGGCTTGGACTGAAGCGGCTGACCGGATTGTGGAAGGTATGGAAGGCGCAATCAATGCCAAGCCCGTa             >  1:456719/1‑143 (MQ=12)                                    gcCGTAATATGACGGTCAGGACGAAGTGAATCCTGGCTCTATTATGCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa            <  2:498115/143‑1 (MQ=17)                                     ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑GGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGACGGCGCACTCACTGCCCAGACCGTAAc           >  2:570296/1‑143 (MQ=18)                                      cGAAATATGCCGGTCAGGATAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct          >  1:341390/1‑142 (MQ=17)                                      cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGCCCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct          >  1:568751/1‑142 (MQ=18)                                              gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATA‑TGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTCTGACtt  >  1:46740/1‑143 (MQ=17)                                                                                                                                        |                                                      GAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACA‑TGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTT  >  NC_000913/1196113‑1196300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑