Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,032	IS1 (–) +8 bp	intergenic (‑110/‑488)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	45 (0.880)	38/284	0.3	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 1293039	0 (0.000)					intergenic (‑117/‑488)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
*	?	NC_000913	1293032 =	0 (0.000)	36 (0.700)	27/284	1.0	100%	intergenic (‑110/‑495)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

CGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293167‑1293032 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGC  <  NC_000913/1979270‑1979129                                                                                                                                                                                                                                                                                        CGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATG                                                                                                                                         <  1:658204/143‑1                      TGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGT                                                                                                                    >  1:94002/1‑143                        ACATGAATCAGGAAGTTGTACCCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGT                                                                                                                  <  2:830989/143‑1                          tTGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATGGTCGGTGTAAATAGGGCTATATGCTGCGTCTTTTCTGGCTAATTTTATGAGAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTAT                                                                                                                <  2:191725/142‑1                          ATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTAT                                                                                                                >  1:749576/1‑143                                       TTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTG                                                                                                   <  2:864652/143‑1                                       TTTCAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTG                                                                                                   <  1:354456/143‑1                                         tttgCCTTACGTTGTGCGAAATTAGCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAG                                                                                                 <  2:418228/139‑1                                                   GTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGT                                                                                       <  2:584984/143‑1                                                    TGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTG                                                                                      <  1:20271/143‑1                                                    TGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTG                                                                                      <  2:94002/143‑1                                                      CTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGC                                                                                    <  1:508131/143‑1                                                            AATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGT                                                                              >  1:808884/1‑143                                                              tcgTCGGTGTAAATAGGGTTATATGCTGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTT                                                                            <  2:814313/140‑1                                                                ATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAACGTGCTGCTGCCAACTTACTGATTTAGTGTATGATGGTATCTTTGAGATGCTCCAGTGTCCTGTGTTTCT                                                                          >  2:593505/1‑143                                                                 gCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTA                                                                         <  1:223265/142‑1                                                                         AATAGGGCTATGTGCCGCGTCTGTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGT                                                                 <  2:678431/143‑1                                                                         AATAGGGCTATATGCCGCGTCTTTTCTGGCTCATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGCTGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGCGGTGCTCCCGTGGCTTCTGTTTCTATCAGCTGT                                                                 >  2:116710/1‑143                                                                         AATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGT                                                                 <  1:556078/143‑1                                                                         AATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAAATAGTTCATGGAGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGCATGATGGGGTGTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGT                                                                 >  2:604575/1‑143                                                                            AGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCC                                                              >  1:730421/1‑143                                                                              ctCTATATGCCGCGTCTTTTCTCGCTAATTTTATCAAACGATATTTATTGGCTGCACAAGGTGATGCTCCCAACTTCCTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTC                                                            <  1:657798/141‑1                                                                                      GCCGCGTCTTTTCTGGCGAATTTTATGAAAAGATATGTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATGGAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAG                                                    <  1:601623/143‑1                                                                                         GCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTA                                                 >  1:149335/1‑143                                                                                                 TCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGG                                         <  1:55864/143‑1                                                                                                 TCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTGCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAGGGG                                         <  2:808884/143‑1                                                                                                     GCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGG                                     >  1:519019/1‑143                                                                                                     GCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCGGTTTCTATCAGCTGTCCCTGCTGTTCAGCTACTGACGGGGTGG                                     >  1:74666/1‑143                                                                                                      CTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGT                                    <  1:611519/143‑1                                                                                                      CTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGT                                    >  2:367265/1‑143                                                                                                          TTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGACAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATGCGCTTTCCCTCCTGTTCACCTACTGTCGGGGCGGCGCGg                                >  2:605801/1‑142                                                                                                                 AAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCA                         >  2:389302/1‑143                                                                                                                        ATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCAC                  >  2:352675/1‑143                                                                                                                                       AAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCG   <  1:710103/143‑1                                                                                                                                        AGGTGATGCTGCCAACTTGCTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTGCTGTTTCGATCAGCTGTCCCTCCTGTGCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGC  <  2:99934/143‑1                                                                                                                                        AGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGCGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTCCCGGCAAAAGCCCCGCCGGACAGCAGCGC  >  2:49214/1‑143                                                                                                                                                                                                                                                                                        CGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293167‑1293032 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGC  <  NC_000913/1979270‑1979129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.