Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	116.7 / NA	39	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/23);  total (16/23)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926                                                                                                                                          |                                                                                                                                            tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGGATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  1:439424/143‑1 (MQ=255)    ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                     >  1:314600/1‑143 (MQ=255)      tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                   >  1:927996/1‑143 (MQ=255)              agacggCTGATATTCTTTGATGTGTCAGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                           <  2:846240/138‑1 (MQ=255)               aaCAGCTGATATTTGTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAc                                                                                                                          <  1:437571/143‑1 (MQ=255)                   gCTGAGATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                      <  1:837746/143‑1 (MQ=255)                       tgatTTGTTGCTGGCGCGGGCGAATTTGTCGTCGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCATCGCCTCTTCTACTTTCCACg                                                                                                                  <  2:296849/141‑1 (MQ=255)                             ggtGCGGGCGCGGTCGAATGTGTGTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc                                                                                                            <  2:727703/141‑1 (MQ=255)                              gtGCTGGCGCGGTAGAATGTGTCGTTGTGCTTGCGGGAAAAGTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGGGACCTGCATTTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                           <  1:51309/142‑1 (MQ=255)                               tGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGGAGGCGACGCGTCGCCTCTTCGACTATCCATGGTCAGCCg                                                                                                          >  2:96863/1‑143 (MQ=255)                                      gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTCCTcca                                                                                                   >  1:660949/1‑143 (MQ=255)                                      gcgGTCGAATCTGTCTTTGTGCTTGCGGTAAAAGTGGACGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca                                                                                                   <  2:75554/143‑1 (MQ=255)                                                 tGTCTTTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc                                                                                        <  1:900400/143‑1 (MQ=255)                                                        gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAGCAAGCGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTACACGGTTCGACGTCCTCTCACACCCCCCGCAGGaaca                                                                                 >  2:855677/1‑141 (MQ=255)                                                           cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCGGTACTCCACCACCATCCGCAGGACGATGt                                                                              >  2:917522/1‑143 (MQ=255)                                                            ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCACCGTACTCCACAAACAACCGCACGAACATGTc                                                                             >  1:509552/1‑143 (MQ=255)                                                               cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTa                                                                          <  1:787153/143‑1 (MQ=255)                                                                gttatacgTGGAAGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                         <  1:849511/136‑1 (MQ=255)                                                                    gaggTGGAAGTACCCGGAGAATTTGTTCGGTGACGGGGTGATCTGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCTCCCTCCGCAGGAAGATGTCGTAATCCt                                                                     <  2:811319/140‑1 (MQ=255)                                                                       gTGGAAGTACCCGGAGGATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  2:456533/143‑1 (MQ=255)                                                                       gTGGAAGTACCCGGAGAATGTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  1:55712/143‑1 (MQ=255)                                                                             gaaCCCGGCGACTTTTTTCGGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCtt                                                            <  1:351049/141‑1 (MQ=255)                                                                              tACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCAACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                           >  1:530827/1‑143 (MQ=255)                                                                                cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         <  1:721037/143‑1 (MQ=255)                                                                                  cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGCGCTCCACCCCCCTCCCCGGGCAGCTGTCGTCCTCCCTTGCGGCtttgcct                                                       >  1:190574/1‑139 (MQ=255)                                                                                        aTTTTTTCGGTGTCGGGGTGAGCTGCTTTTCTCCGTGTTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTa                                                 <  2:472177/143‑1 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                               >  2:783797/1‑143 (MQ=255)                                                                                           gttccGGTGACGGGGTGCTCTGCTGTTCCCCGTGGTTGGTACGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                              <  2:819462/139‑1 (MQ=255)                                                                                                 gTGGCGAGGTGAGCTGCATTCCACCGTGATTGATGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  2:813638/143‑1 (MQ=255)                                                                                                 gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  1:278212/143‑1 (MQ=255)                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  >  1:268547/1‑106 (MQ=255)                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  2:268547/106‑1 (MQ=255)                                                                                                             tCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                            <  2:497365/143‑1 (MQ=255)                                                                                                               tGCCTTTCACCGTGATTTTGATTCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCCCCAACCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg                          <  2:75140/143‑1 (MQ=255)                                                                                                               tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCATGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGAACAAACACTCTTTGAAACg                          >  1:142142/1‑143 (MQ=255)                                                                                                               tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCGCCATCCGCAGGAAGATGTCGTAATCCTGTGGCGCTTTCAGTTCGGTGTCGAGCAAAAACTCTTTGagtcg                          >  1:415988/1‑139 (MQ=255)                                                                                                                              ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACCCTTTGAAACGCCACGCCCCGGCaat           >  1:449933/1‑142 (MQ=255)                                                                                                                                    tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAGCGCCATGCCCCGGTCAAGccccg     >  2:331959/1‑141 (MQ=255)                                                                                                                                       aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCCACTCGGTATCGAACAAACACTCTTTAAAACGCCATGCCCAGGTAAAGCCCtagct  >  2:366509/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                            TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑