| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,489,152 | C→T | S6N (AGC→AAC) | yfdE ← | acetyl‑CoA:oxalate CoA‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,489,152 | 0 | C | T | 97.8% | 133.1 / ‑5.6 | 45 | S6N (AGC→AAC) | yfdE | acetyl‑CoA:oxalate CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (21/23); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
GTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACA > NC_000913/2489014‑2489290 | gtgCGGGTATCAGCACCATTACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTc < 2:69354/143‑1 (MQ=255) cGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCatt > 1:3846/1‑143 (MQ=255) tATCCTCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTAttt < 2:828121/143‑1 (MQ=255) tATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTAttt < 2:681406/143‑1 (MQ=255) catcaCCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATCTGTc > 2:167178/1‑143 (MQ=255) atcaCCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGGTCCGAAAGTTACATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTcg > 1:433245/1‑142 (MQ=255) tcaCCAGGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCAt < 1:712257/143‑1 (MQ=255) ccATGCGCCGGCGGCGCAACTGTAATTACCCTTGCGCTCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTg < 2:132441/143‑1 (MQ=255) ccGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACAGGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGc < 1:153522/143‑1 (MQ=255) gcTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGaa < 1:738704/143‑1 (MQ=255) tCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGaaaa < 1:191443/143‑1 (MQ=255) aaCTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGaaaaaa < 1:749447/143‑1 (MQ=255) aaCTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGaaaaaa < 1:76253/143‑1 (MQ=255) aaCTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGATTAAGCATTAaaaaaa > 2:309059/1‑143 (MQ=255) aCTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTACGAAAGGTACATTAAGGACATGTGTCATATCGAGAACTAATAAGCCTACAAACGGCCCTTTGTTTTCATTATTTGGCATTTGCGTATGCCTCGAAAAAAg > 2:587472/1‑143 (MQ=255) ttAATTACCCTTGCGCCCATATTACAACGAAGTTGAGTTCCGAAAGGCCCCTTAAGGCCATGTCTCATATCGACAACTAATAAGCATTCAAACGGCCCTTTGTTTTCATTATTTGTTATTTTCTTATGCCTTGAAAAAAGCCg > 1:500134/1‑143 (MQ=255) ttGCGCCCATATTGCCAAGGCGTGGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGaaaa < 1:712718/143‑1 (MQ=255) tggcgcCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGaaaa < 1:537048/141‑1 (MQ=255) agatgaCAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTc < 1:611631/138‑1 (MQ=255) atTACAACGAGGTTGAGTTCCGAAAGGTCCATTCAGGACATGTGTCATATCTATAACTCATAACCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCGTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCgg < 2:679786/143‑1 (MQ=255) aCAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGGCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCAt > 1:506378/1‑143 (MQ=255) cAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCAtt > 1:545694/1‑143 (MQ=255) gTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTaa > 1:810028/1‑143 (MQ=255) ttGAGTTCCGAAAGGTCCATTAAGGACATGTGGCAAATCGATAACTAGTAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTaaa > 1:882122/1‑143 (MQ=255) ttCCGAAAGGTCCATTAAGGTCATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTGTCATTATTTGTCATTTGCTTACGCCTTGAAAAAAGCCGAAGGGGAAAAGACACTTCGGCATTTATCCAAAATCaa > 2:481668/1‑143 (MQ=255) ttCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCaa < 1:100438/143‑1 (MQ=255) tCCGAAAGGTCCATTAAGGACATGGGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAAt < 2:614959/143‑1 (MQ=255) aaaGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACa < 2:666807/143‑1 (MQ=255) aGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAACATCAATAACATg < 2:512044/143‑1 (MQ=255) gtgtCATAGCGATAACTAATAAGCCTTCAAACGGCTCTTTGTGTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAg < 2:71876/143‑1 (MQ=255) atatCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTCTGCCTTGAAAAAAGCCGAAGATGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGAc > 1:63031/1‑143 (MQ=255) atatCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCACTAACATGATTAATTTTGAAACCAGTCAAc > 1:643516/1‑143 (MQ=255) atatCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGAc < 2:853465/143‑1 (MQ=255) cGATAACGAATCAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGAc < 2:921827/143‑1 (MQ=255) taataaGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTGTGTAAAATCAATAACTTGATTTATTATGAAACCAGTCGACCGACATaaaca > 1:276975/1‑141 (MQ=255) taataaGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGCCATAAATc > 1:156399/1‑143 (MQ=255) ttCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGCAGGTGAAAAGACACTTCGCCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCACCAAGGGa > 1:650280/1‑143 (MQ=255) tCAAACCGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAATGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAg < 1:181936/143‑1 (MQ=255) cAAACTGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACGTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGc < 1:846404/143‑1 (MQ=255) cAAACCGCCCTATGCTTTCATGATTGGTCATTTGCTTATGCCTTGAAAAAATCCGAAGGTGAAAAGACACTTCGGCATTTACGTAAAATCCATAACAGGAGGAATGACGAAACCAGTCGACTGACATAAATCCACACGGGAGc < 2:276085/143‑1 (MQ=255) aaCGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGCAGCCg > 1:610219/1‑143 (MQ=255) aaCGGCCCTTTATTTTCATTATTTGTCATTTGCTTATGCCTTGACAGAAGCCGAAGGTGAAAAGACACTTCGGCATTTGTGTAAAATCAAAAACATGAGTAATCATGAAACCAGTCGACAGAGATCAATCAACAAGGAAGACg > 2:536808/1‑143 (MQ=255) cTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCGCAAGGGAGCCGTgacgac > 1:23716/1‑143 (MQ=255) cTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTgacgac > 1:543073/1‑143 (MQ=255) tttGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACGAGGGAGCCGTGACGACa > 2:308518/1‑143 (MQ=255) | GTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACA > NC_000913/2489014‑2489290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |