Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	98.0%	133.9 / NA	50	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (25/23);  minor base A (0/1);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041258‑3041533                                                                                                                                             |                                                                                                                                        aTAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCATCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCACTGACGCTGATGACGCTTTATGACTGGGCGCGTg                                                                                                                                       >  1:782498/1‑143 (MQ=255)       ctctgaCCAGTGGTCATTAGTACGCCGTGAGGCAACTCGGGGCTCTATTATGGCTTGTACACCTGTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccacca                                                                                                                                 <  2:15500/138‑1 (MQ=255)        ttttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAt                                                                                                                                >  2:201558/1‑143 (MQ=255)        ttttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTACTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGCTGACTGGGCGCGTGCCACCAt                                                                                                                                >  1:132819/1‑143 (MQ=255)          ttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCTTCCCCGTCAGCCTACGGCTTCTTCCCGTTTATCCCGGACGCTGATTACGCTTGGTGACTGGGCGCCTCCCCCCATTa                                                                                                                              >  1:733875/1‑143 (MQ=255)              cATTAGGCGTTGATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTCGATGACTGGGCGCGTGCCACCATTCCtcg                                                                                                                          >  2:160526/1‑141 (MQ=255)                    tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTCGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                                    >  1:454777/1‑143 (MQ=255)                      aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATAACTGGCGCGGACa                                                                                                                  >  1:243234/1‑143 (MQ=255)                       ttAACACGCCGTGAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAg                                                                                                                 <  1:166803/143‑1 (MQ=255)                          aTACGCCGTTAAGCAACTCAGGACTCTATTAGGGCTTTTACACCTTTTCCTCCCCGTCAGGCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGa                                                                                                              <  2:338482/143‑1 (MQ=255)                              gCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCGCGTTTACCGCTGACGCTGATGGCGCTTGATGACTGGGCGGGTGCCACCATGACTGGCGCGGAGGGCGaaaga                                                                                                          >  1:234334/1‑141 (MQ=255)                                 gTTAAGCACCTCGGCTCTCTATTATGGCTTTTACTCCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGGTGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatat                                                                                                       <  2:530959/143‑1 (MQ=255)                                 gTTAAGCAACTCAGGACGCTATTATGGCTTTTACACCTTTGCCTCCCCGTCAGCCTACGGCTTCGGCCCGTCTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatat                                                                                                       <  1:549131/143‑1 (MQ=255)                                     atcAACGCAGGGCGTTATTATGCCTTTTGCACCTTTTCCTCCCCTTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGTTTGATTACTGGGCTCGTGCCACCATTACTGGCTCGGACAGCGAAAAATATATGc                                                                                                   <  2:504663/141‑1 (MQ=255)                                      gCAACTCCGGACTCTATTATGGCTTTTACACCCTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCa                                                                                                  <  2:54001/143‑1 (MQ=255)                                       cAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACCGCGCACAATATACGCAg                                                                                                 >  2:845326/1‑143 (MQ=255)                                        aaCTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAgg                                                                                                <  1:201558/143‑1 (MQ=255)                                          cacAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGGCAGCGAAAAATCTATGCAGGGt                                                                                              >  1:585713/3‑143 (MQ=255)                                             actaCTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACCCTGATGACGCTTTATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg                                                                                           <  1:612219/140‑1 (MQ=255)                                                    tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGGAAGAAATATGCGGGGTGAGGTGAcag                                                                                    >  1:500873/1‑143 (MQ=255)                                                    tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGCAAAATATATGCAGGGTCAGGTGAcag                                                                                    >  1:748631/1‑143 (MQ=255)                                                           gCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGt                                                                             <  1:811802/143‑1 (MQ=255)                                                                 acacCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCCGATGACGCCTGAGGAGTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGAAAGAGCAGGTGCAAGCAGATATCAGcaa                                                                       >  2:919253/1‑141 (MQ=255)                                                                   acCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGAGAGCGAAAAAGATCTGCAGGGTCAGGCGACAGCAGATGACCGCCAgc                                                                     >  2:739826/1‑142 (MQ=255)                                                                      ttgtcccccCCTTCAGCCTACGGCTTCTGCCCGTCTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                                                                  <  1:822467/140‑1 (MQ=255)                                                                      cttccctccCCGCCTGCCTACGGCTTCTGCCCGTTTACCGCTGCCTCTTCTCACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAATAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                                                                  <  2:312614/139‑1 (MQ=255)                                                                       tttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGc                                                                 <  2:231158/143‑1 (MQ=255)                                                                             ccccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTTATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACACCGATGTATGGATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATCGCaggaga                                                           >  1:773283/1‑143 (MQ=255)                                                                               ccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                         <  2:132819/143‑1 (MQ=255)                                                                                      ccTACCGCTTCTGCCCGTTCGCCGCTGACGCTGATGACGCTTGATGACTGCGCGCGTGTCTCTGTTAGTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCTGCCAGATGGCAGAAGATCAGCACCt                                                  <  2:803041/143‑1 (MQ=255)                                                                                       gtACGGCTTCGGCCCGTGTCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTg                                                 <  1:517371/142‑1 (MQ=255)                                                                                          cGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCGGCTc                                              >  2:444009/1‑143 (MQ=255)                                                                                           gtcTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCGACCATTACTTGCGCAGACAGCGACAAATATATGCAGGGTCAGGTGACAGCAGCAATAAGCCAGATGACAGAAGATACGCAACTGCTct                                             >  2:513493/3‑142 (MQ=255)                                                                                                 tGCCCGTTTACCGCTGGCGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATGACTGGCGGGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc                                       <  2:733875/143‑1 (MQ=255)                                                                                                 tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc                                       <  2:706943/143‑1 (MQ=255)                                                                                                   cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCAGGCCAt                                     >  1:70816/1‑143 (MQ=255)                                                                                                        ttACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGa                                <  1:528252/143‑1 (MQ=255)                                                                                                         tACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCACTACTGGCGCGGGCAGCGCAAAATATAAGCAGGGGCAGGTGGCAGCAGATGTCAACCAAATGGCAGAACATCAGCACCTGCGCGCCGCGCATGGCggc                               >  2:174604/1‑141 (MQ=255)                                                                                                            cgctgacgctgaTGACGCTTGCTGACTGGGCGCGTGCCACCATTACTGGCGCGGCCAGCGAAACATCTATGCTGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCCCCTGCTCGCCGCCCATTGCgccgcc                            >  1:346483/1‑143 (MQ=255)                                                                                                            cgctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGcc                            >  1:788367/1‑143 (MQ=255)                                                                                                               tgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaaa                         <  1:796280/143‑1 (MQ=255)                                                                                                                     tgatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGAAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAgctcaa                   >  2:542667/1‑138 (MQ=255)                                                                                                                        ggaCGCTTGATGACTGGGCGCGGGCCACCATTACTGGCGCGGAGAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg                <  1:845326/142‑1 (MQ=255)                                                                                                                          aCGCGTGATGAATGGGAGCATGCAACAAGTAGTGGCGCGGACAGCGAAAAATATAGGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgtg              <  2:204933/143‑1 (MQ=255)                                                                                                                            gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATCTGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAAGTGGa            >  2:578587/1‑143 (MQ=255)                                                                                                                                  tgaCAGGGCGCGTGACACCATGACTGGCGCGGACAGCGCAAAATATATGCACGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt      <  2:677933/143‑1 (MQ=255)                                                                                                                                   gaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAttt     >  2:431550/1‑143 (MQ=255)                                                                                                                                    aCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCCGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCACTTTa    >  2:610541/1‑143 (MQ=255)                                                                                                                                     cTGGGCGCGTGCCACCATAACGGGCGCGGAGAGCGAAAAATATAGGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTAc   <  1:161123/143‑1 (MQ=255)                                                                                                                                      tGGGCGCGTGCCACCATTACTGGCGCCGACAGCGAAAAATATATGCAGGGTCAGGTGACCGCAGATGTCAGCCAGATGGCAGAAGATCAGGACCTGCTCGCCGCCCATTGCGACGCCAAAGGTACAAAGTGGAGCCATTTGCg  >  2:786199/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                        ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041258‑3041533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑