Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,792	G→T	E296* (GAA→TAA)	sdhA →	succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,792	0	G	T	100.0%	75.7 / NA	27	E296* (GAA→TAA)	sdhA	succinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/15);  total (12/15)

GGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCC  >  NC_000913/756654‑756931                                                                                                                                           |                                                                                                                                            ggCGTATTGGTTAGCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAAt                                                                                                                                         <  1:352566/143‑1 (MQ=255)                       gCCGTGGTGAAGTCGGGTGTCTGCAGAACAAACATGGCGAACGTCGTATGGAGCGTGATGCGCCGAACGCCAAAGTCCGGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGAt                                                                                                                   <  2:518697/143‑1 (MQ=255)                        ccGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGagg                                                                                                                  >  1:907316/1‑141 (MQ=255)                           tggtgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGCTCTAAATCCGTGAAGGTCGCGGCTGTGATGGTc                                                                                                               >  2:473952/1‑143 (MQ=255)                           tggtgAAGGCGGTTATCTGCTGAACAAACACGGCGACCGCATTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCCTCATGCTCTAACTCCGTGAAGGTCGCGGCTGTGATGGTc                                                                                                               >  2:330247/1‑143 (MQ=255)                               gagggcggcTATCTGCTGAGCAAACATGGCGAACGTTTTAGGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTg                                                                                                           <  1:150465/140‑1 (MQ=255)                                aaGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTgg                                                                                                          <  1:386607/143‑1 (MQ=255)                                             gctgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCCCACGCga                                                                                             >  2:873078/1‑143 (MQ=255)                                               tgAACGAACCGGGCGAACGTTTTATGGAGCGTTAGGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaa                                                                                           <  1:419481/143‑1 (MQ=255)                                               tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaa                                                                                           <  1:810397/143‑1 (MQ=255)                                               tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTCGGGACCACACGCgaac                                                                                           >  2:175957/1‑142 (MQ=255)                                                    aaacaCGGCGAACGATTAATTGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactgaa                                                                                      <  1:573253/143‑1 (MQ=255)                                                                 gTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCACCGTGATCTAAATCCGTGGAGGTCCCGGCTCACAAGGTCCGTGGGGGGCTCACGCGAAACTGGAACTCGGTCGCCTg                                                                         >  1:721681/1‑143 (MQ=255)                                                                          ggCGTCACGCGCCGACCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaa                                                                <  2:65853/142‑1 (MQ=255)                                                                          aGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaa                                                                <  1:378898/143‑1 (MQ=255)                                                                                  gcgcCCGACGCCAAATACCTGGCTGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGa                                                        <  2:496947/143‑1 (MQ=255)                                                                                                          ggCCGTGAGGTGGGTGCGCGTGCCATCAGGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGa                                <  2:272757/143‑1 (MQ=255)                                                                                                           gCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAg                               <  1:839275/143‑1 (MQ=255)                                                                                                                gccGTGGTTTCGCGTTCCATCATGATCTAATTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGACGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTc                          <  1:266542/141‑1 (MQ=255)                                                                                                                   gTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTGCTCGAGTCCCGTCTGCCGGGTATCCGGCAGCGTTCCCg                       >  1:575195/1‑143 (MQ=255)                                                                                                                     cgTTGCGCTTCCCGTCATGATCTGAACCCGTGAAGTTCGCGCCTTTGATGGTCCGTGTGGCGCACACGCGAAACTCAAACTCGATCACCTTGCTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTCGAGCTTTTCCTTa                     <  2:767970/142‑1 (MQ=255)                                                                                                                        tGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCAGGAGCTTTCCCGTAACt                  >  2:683382/1‑143 (MQ=255)                                                                                                                           gcgtcgcatcttGATCTACATCCGTGAAGGGCGCGGCTGTGATGTTCCGTGGGGGCCAGACGCGAAACTGATACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg               <  2:762004/137‑1 (MQ=255)                                                                                                                           gcgTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGCAGTTCTCGAATCCCGTCTGCCGGGTATGCTGGAGCTTTCCCGTACCTTCg               >  2:664373/1‑143 (MQ=255)                                                                                                                                 catcatGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACg         >  2:210624/1‑143 (MQ=255)                                                                                                                                       gATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGGGCTTTCCCGTACCTTCGCTCACGTCGATc   >  1:394996/1‑143 (MQ=255)                                                                                                                                        aTCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGAGCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATcc  >  1:922287/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                            GGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCC  >  NC_000913/756654‑756931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑