| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,173,361 | Δ2 bp | 100% | pseudogene (917‑918/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 94.3% | 144.2 / 0.7 | 35 | pseudogene (918/1358 nt) | gatC | galactitol‑specific PTS enzyme IIC component |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (15/18); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 94.3% | 140.7 / 0.3 | 35 | pseudogene (917/1358 nt) | gatC | galactitol‑specific PTS enzyme IIC component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (15/18); minor base T (0/2); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTG > NC_000913/2173223‑2173497 || ttgcACGAGGCACGGCGACCGCCAGCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc < 2:284443/140‑4 (MQ=255)ttCCACGATGCACGGCGACCGCCATCGCCACGAAGACGCCGATGGTGGCAAGATCGCCAAACGGCCGCACCGGATTACCCGGCACAAAAACAGC‑AATCAAAATGGTGAGCGGCAGAAAAACCAGCCTCACCGATaccgccgcc > 2:808366/1‑140 (MQ=255)ttCCACGATGCACGGCGACCGCCATCGACACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc < 2:657491/143‑4 (MQ=255) cACGATGCACTGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGC‑AATTACAATGGTGAGAGGGAGAAAAATCAGGCGTGCCGATaccaccgccgga > 2:754746/1‑137 (MQ=255) cccGGCGACGGCCATCGCCACGAAGAAGCGGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAACATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCca < 2:786111/141‑1 (MQ=255) aCGGCGACCGCCATCGCGACGAAGAGGCCGATGGGGGCAAGAGCGCCAAACGGCAGCACCTGATTACCCGGCACACCAACAGC‑AATTAAAACGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcag < 2:575422/143‑1 (MQ=255) ggCGACCGCCATCGCCACGAAGACGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca < 1:763292/143‑1 (MQ=255) ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCTCCCAACAGC‑AATTAAAATGGTGAGTGGGATAAAACTCAGGCTTGCCCATACCA‑‑CCGCCGTATGTCCcagca > 1:565927/1‑143 (MQ=255) ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca > 1:71925/1‑143 (MQ=255) ccATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCCACGcc > 1:158871/1‑143 (MQ=255) accgagaagCCGATGGTGGCAACATCGCCAAACGGCAGCACGTGCGTCCCCGGCACACCAACGGC‑AATTAAAATGGTGGGTGGGATACACAGCCGGAAAGCCGTGAC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg < 1:937395/139‑1 (MQ=255) aCGAAGAAGCCGCTGGTGGCAAGATCGCCGAGCGGCAGCACCGGATTACCCGGCACACAAACAGC‑AATTAACATGGTGAGTGGGAGAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg < 1:487227/143‑1 (MQ=255) gaagaaGCCGATGGCGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AACTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc < 1:333235/143‑1 (MQ=255) ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg < 2:807366/143‑1 (MQ=255) ccGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg < 1:168156/143‑1 (MQ=255) cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg < 1:827779/143‑1 (MQ=255) ctcgcggCACGCTCGCCAGTCGGCACCACCTGATTACCCGGCCCACAAACAGC‑AATTAAAATGGTCAGTGGGATGAAACTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGTATCAAGGCCAATCAGGaa < 1:404959/138‑1 (MQ=255) aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa < 1:203558/143‑1 (MQ=255) aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAACGGCGAGCGCGTTACGTATCATGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa > 1:458803/1‑143 (MQ=255) gCAAGATCGCCAAACGGAAGCACCTGATTACCCGGCACACGAACAACAAACCACAATGAT‑ATTTGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTg < 1:979187/143‑1 (MQ=255) aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGCTCACGGCCAATCCGGAACTCCTGAccgc > 2:107792/1‑143 (MQ=255) aaCGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt < 1:655190/143‑1 (MQ=255) aaCGCCCGCACCTCATCACCCGGCACACCACCAGC‑AATTAAAATGCTCACTGGGATAAAAATCAGGCTCGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt < 1:174218/143‑1 (MQ=255) aCGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTAGCCGATTC‑‑CTGTGCAGTACAGCCCAACAGCAACGCCGGATCAAGGCCAATCAGGATCTCCTGACCGCCGAACTTc < 2:700832/143‑1 (MQ=255) cTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc > 2:577861/1‑143 (MQ=255) aTTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACGTCGCCCGTAAACGAc > 2:1954/1‑143 (MQ=255) ccGGCACACAGAAAGC‑AGTTTAAATGGTGAGTGGGATAAAAATCAGGCTTGCCTATACAA‑‑CCGCCGTGTGTCCCAGCAGCAACACCGGATCAAGGCAAATCGGGAACTACTGACCACCGAAATACGCCTATAAAAGAATAcaa > 2:355323/1‑141 (MQ=255) acacaAACAGC‑AATTAAAATGGTGAGTGGTATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCGGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTg < 2:972858/143‑1 (MQ=255) cacgAACAGC‑AATTAAAATGGTGAGTGGGAGAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGc < 2:278801/143‑1 (MQ=255) aaaCAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCCACGCCGGATCAAGGCCCATCCGGAACTCCTGACCGCCGCACTTCGCCCGTAAACGGCCACCACCCTGccca > 1:431663/1‑140 (MQ=255) ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc > 1:809807/1‑143 (MQ=255) tAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg > 2:45384/1‑143 (MQ=255) aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGtt > 1:503385/1‑143 (MQ=255) tGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACCAGGCAATGACATCCTCCTTGCTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGGTaaa > 1:455529/1‑143 (MQ=255) ggTGGGATAAAAATCAGGCTTGCCGGCACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatc < 1:447980/142‑1 (MQ=255) aTAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCCGCACTGCCGGACCAAGACCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgat < 1:454480/143‑1 (MQ=255) aaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt < 1:731651/143‑1 (MQ=255) ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTCAATCACCCGt > 2:478555/1‑143 (MQ=255) cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGACTGTAAACGACTACGAGCCTGCTTCGCGATGGGCGTTAAACCATCCATGATGGGGTTAACCATCCGTg > 2:780573/1‑143 (MQ=255) || TTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTG > NC_000913/2173223‑2173497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |