| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,085 | Δ1 bp | 100% | coding (2069/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | . | 93.8% | 192.5 / 0.8 | 48 | coding (2069/2664 nt) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (27/18); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124951‑125221 | caGCCACATCCCGTCGCTTACTATCCCGAACTGTATCTCTTACGGCCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCt < 2:200146/143‑1 (MQ=255) aTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACAACCCTGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAACCAAGAGAACGCTTACTACTACA‑CACTACGCTGAACGaa > 2:599592/1‑143 (MQ=255) ttCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaaa < 1:392847/143‑1 (MQ=255) tCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTAc < 1:957638/143‑1 (MQ=255) tATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGcc > 1:759000/1‑143 (MQ=255) cGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGCGCGTATGTACGGTGAAAAACAAGAGAACGTTTCCTTAT‑CCTCACCACGCTGAACGAAAACTACCACATGCCGGCaa > 1:764588/1‑143 (MQ=255) cGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCaa > 2:576483/1‑143 (MQ=255) cGAACTGTATCTCTTACGACCCGGCTTACGCTTAAGAAGTTCATGCCATCATGCATGACGGTCTGGAGCGTGAGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAACCTACGACATGCCAGCaa > 2:442363/1‑143 (MQ=255) gAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGCCTACTACTACA‑CACTACGCTGAACGCAAACTACCACATGCCGGCAAt > 2:341256/1‑143 (MQ=255) cggTAGCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGcc < 1:221956/141‑1 (MQ=255) tGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCg < 1:640956/143‑1 (MQ=255) tATCCCTTACGACCCGGCTTACGCGTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTG‑AAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGaa < 2:781697/143‑1 (MQ=255) tctTGCGACCCTGCTCAGGCTTACGAAGCAGCGGTCATCATGCATGACGGTCTGGAGCGTATGGACGGCGAAAAACAAGAGAACGTATACGACTGC‑GCGCTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGt < 1:91337/143‑1 (MQ=255) cagccccGGCGTGCGCGGACGAGGTGGCTGTCATCAGGCATGGCGGGCTGGGGCGTAGGGACGGTGAAAAACAGGAGAACGTTTACTACTGCA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTg < 1:1864/139‑1 (MQ=255) cctggttacgcttacgTAGTTGCTGTGATAATGCATGACGGTCAAGAGTGGAGGGAGGGTGAAAAACAAGCGCAGATTTACGACCACA‑CGCTAGGCTGAACGAAAGCTACCACATGCAGGCAATGCCGGAAGGTGCTGAGGaa < 2:341497/138‑1 (MQ=255) ccGGCTGACGCCTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGTGTAAAAAAAAAAAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACGACATGCCGGCAGTGCCGGAAGGTGCTGAGGaa < 2:516336/143‑1 (MQ=255) gcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGGATGTACGGTGAAAAACAAGAGAACGTTTACTGCTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGt > 1:420156/1‑143 (MQ=255) gcttacgAAGTTGCTGTCATCATGCATGACGGGCTGGCGCGTATGTACGGTGAAAAACAAGAGAACGCTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGCAGGGGCTGCGGAAGGTATcccg > 2:124277/1‑141 (MQ=255) cttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTa < 1:262646/143‑1 (MQ=255) cgtacgAAGTTGCTGCCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTa < 1:967381/141‑1 (MQ=255) ttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCAGTaa > 2:703166/1‑143 (MQ=255) gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCCGAGGAAGGTATCCGTAAAGGt > 2:506914/1‑143 (MQ=255) aGTTGCTGTCATCATGCATGCCGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTAC‑CCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTAt < 1:684829/143‑1 (MQ=255) aGTAGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTAt < 1:519624/143‑1 (MQ=255) tcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCg > 1:126795/1‑143 (MQ=255) caggactgACGGTCTGGAGCGTAGGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGa < 1:832802/137‑1 (MQ=255) ccatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGTAACCCCCCACATTCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAAc < 1:139082/142‑1 (MQ=255) catgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaccg > 1:650274/1‑140 (MQ=255) gACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAtt > 1:521130/1‑143 (MQ=255) cTGGAGCGTCTGTACGGTGACAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGCAAACTACCACATGCCGGTAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGt < 1:193417/143‑1 (MQ=255) tGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACAATTGAAGGTa > 2:20636/1‑143 (MQ=255) ggAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCAGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGGAAAGGTATCTAAAAACGCGAAACTATTGGAAGTAg > 2:128682/1‑143 (MQ=255) gAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTGGAAACTGTTGAAGGTAGc > 1:671624/1‑143 (MQ=255) gcctATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaa < 1:443208/140‑1 (MQ=255) cGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaaa < 1:539988/143‑1 (MQ=255) gTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGACGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAg > 1:777629/1‑143 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCCACAAACTCGAAACCATTGACGCTCGCAAAgggcaag > 2:747354/1‑138 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTACA‑CAATACGCTGAACAAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAAACAGGTAAATGTGGAGGTATCTACAAACTCGACACTATAAAAAGCAGCACAGCTAAAg > 2:475613/1‑143 (MQ=255) ggTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTGGAAACTATTGAAGGTAGCAAAGGTAAAGtt > 1:965567/1‑143 (MQ=255) gTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAAATACCACATGCCGGCAGTGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTGGCAAAGGTAAAGTTc > 1:140577/1‑143 (MQ=255) tGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCa > 2:853658/1‑143 (MQ=255) gAAAGACAAGAGAACGTTTCCTACGACA‑CACGACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAg < 2:253495/143‑1 (MQ=255) gAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACAACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACACACTCGAAACTATTGAAGGTAGCAAAGCTAAAGTTCAg > 1:428123/1‑143 (MQ=255) aaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctg > 2:132995/1‑143 (MQ=255) agaACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGCAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAACGGTAAAGTTCAGCTGCTCgggt > 1:811789/1‑141 (MQ=255) gaACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAGGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGACGGTAGCAAAGGCAAAGTTCAGCTGCTCGGCTc > 1:619978/1‑143 (MQ=255) tactacA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTATATc > 2:803334/1‑143 (MQ=255) tactacA‑CACTACGCTGAACGAAAACTACCACATGCCGGAAAAGCGGGAAGGGGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTGCTATc > 1:773664/1‑143 (MQ=255) | CAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124951‑125221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |