BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,287,423:1	+T	100%	intergenic (‑52/‑341)	acs ← / → nrfA	acetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,423	1	.	T	95.0%	343.5 / 10.7	120	intergenic (‑52/‑341)	acs/nrfA	acetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase
Reads supporting (aligned to +/- strand): ref base . (4/2); new base T (66/47); total (71/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

ATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑G‑T‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCAT  >  NC_000913/4287281‑4287561

aTTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑g                                                                                                                                               <  2:671143/143‑1 (MQ=255)

 ttgttgATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑G‑t                                                                                                                                             >  2:217476/1‑143 (MQ=255)

   gttgATACATTGGCGCGTGGTGCTGAGGGTTTATCAGGCAACGGTCGGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑G‑tta                                                                                                                                           <  1:527751/143‑3 (MQ=255)

   gttgATACATCGCCTCGTACTGCTGGGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑G‑tta                                                                                                                                           <  1:784696/143‑3 (MQ=255)

    ttgATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑G‑ttag                                                                                                                                          <  1:901006/143‑4 (MQ=255)

      gATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAGTTTGGCTCATGCTCTTGTTCTGCGTGTAGGATGTTGCTACTAAGTGGCATAAGCGATACAT‑G‑ttaggg                                                                                                                                        >  2:474520/1‑138 (MQ=255)

      gATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTGGTGAATTTGGCTCATGCTTGTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑G‑ttaggg                                                                                                                                        >  2:490334/1‑138 (MQ=255)

       aTACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑Agg‑gg                                                                                                                                      >  2:709514/1‑143 (MQ=255)

           aTCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTAtt                                                                                                                                  >  2:103283/1‑143 (MQ=255)

            tCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTg                                                                                                                                 >  1:741693/1‑143 (MQ=255)

            tCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTAGAAT‑GTT‑AGG‑GGTATTg                                                                                                                                 >  1:241025/1‑143 (MQ=255)

            tCGCCTCGTACTGCTGAGGGTTTATCAGCCAACGGCCTGCGGTGTGGG‑CAGGAGTGCGGGGGTTGGGAATGTGGCTCATGCTTTTGTTCTCCTTGGCGGATGTTGATAATATGTGGCATAAGCGTTAAAT‑G‑T‑TGGTGGTATTg                                                                                                                                 >  1:211690/1‑143 (MQ=255)

               ccTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTGAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCa                                                                                                                              >  1:58745/1‑143 (MQ=255)

                cTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTggacg                                                                                                                             >  2:834622/1‑140 (MQ=255)

                 tCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGt                                                                                                                            <  1:378599/143‑1 (MQ=255)

                  cGTACTGCTGAGGGTTTATCACGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAACTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGtt                                                                                                                           <  1:4324/143‑1 (MQ=255)

                     aCTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGGATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATCT‑T‑ACG‑GGCATTGGCAGCTTTg                                                                                                                        >  1:273488/1‑143 (MQ=255)

                     aCTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGGTTTg                                                                                                                        >  1:976621/1‑143 (MQ=255)

                      ctgctgAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGt                                                                                                                       <  2:962436/143‑1 (MQ=255)

                       tgctgGGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGGGTGTATTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGtt                                                                                                                      <  2:799906/143‑1 (MQ=255)

                       tgctgAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTGTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGtt                                                                                                                      >  2:677756/1‑143 (MQ=255)

                         ctgAGGGTTTATCAGGCAACTGTCGGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCATTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTa                                                                                                                    <  1:519309/143‑1 (MQ=255)

                         cggAGGGTTTTACAGGCAACTGTCTGCGGTGTTGGCCACTGCTGGT‑TGTGTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTa                                                                                                                    <  2:695556/141‑1 (MQ=255)

                          tgAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CGGGAATGGTGTGTTTGTGAATTTGGCTCGTGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAACT‑GTT‑CGG‑GGTCTTGGCAGTTTTGTTCAg                                                                                                                   >  1:631817/1‑143 (MQ=255)

                          tgAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGTCATAAGCGTGCAAT‑G‑TAAGG‑GGTAGTGGCAGTTTTGATTAg                                                                                                                   >  2:60373/1‑143 (MQ=255)

                          tgAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGTATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAg                                                                                                                   >  1:930733/1‑143 (MQ=255)

                            aGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTa                                                                                                                 >  1:442991/1‑143 (MQ=255)

                               gTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTAttt                                                                                                              <  2:328403/143‑1 (MQ=255)

                                  tATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAGTATGAGGCATAATCTGTACAT‑GCT‑AGG‑GTTATTTGCAGTTTTGTTTAGTATTTggg                                                                                                           >  2:242652/1‑143 (MQ=255)

                                  tATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTggg                                                                                                           <  1:53689/143‑1 (MQ=255)

                                  tATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGACAGTTTTGTTTAGTATTTggg                                                                                                           >  2:807461/1‑143 (MQ=255)

                                   aTCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATAGGCAGTCTTGTTTAGTAGTTGGGc                                                                                                          >  2:963117/1‑143 (MQ=255)

                                        gCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAg                                                                                                     >  1:21419/1‑143 (MQ=255)

                                        gCAACGGTCTGCGATGTTGG‑CAGGAATGGTCAGTTTGTGAATTTGGCTCATGCTTTTGTTCTACTTGTAGGATGATAATAATAGGTGGCATAAGCGTTAAAG‑GTT‑AGG‑GGTAGTGGCAGTTTTGATTAGTATTTGGGCGACAg                                                                                                     >  1:326098/1‑143 (MQ=255)

                                          aaCGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCCTGCTTTTGTTCTCCTTGGAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAt                                                                                                   <  1:470595/143‑1 (MQ=255)

                                           aCGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATc                                                                                                  >  1:351336/1‑143 (MQ=255)

                                            cGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCa                                                                                                 <  1:709514/143‑1 (MQ=255)

                                              gTCTGCGTTGTTGT‑GTGTGATTGTGTGTTTGTGGTTTTTGCTCAGGCTTGTGTGCTTCTTGTAGTGTGTTAATGATATGTGGCATATGCCTTAAGT‑G‑TGATG‑GGTATGGGCAGTTTTGTTTAGTATTTTGTCGACAGATCACg                                                                                               <  2:925390/143‑1 (MQ=255)

                                                 tGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GCTATTGGCAGTTTTGTTTAGTATTTGAGCGACAGATCACGcga                                                                                            >  2:856492/1‑141 (MQ=255)

                                                 tGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATCTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGAGACAGATCACGCaa                                                                                            >  2:593469/1‑143 (MQ=255)

                                                    gATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTTGCATAAGCGTTAAAT‑GTT‑AGG‑TGTATTGTCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAg                                                                                         <  1:700588/143‑1 (MQ=255)

                                                      tGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAAGATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTa                                                                                       >  2:744818/1‑143 (MQ=255)

                                                       gTTGG‑CAGGAATGCTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAg                                                                                      <  1:818082/143‑1 (MQ=255)

                                                         tGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGaa                                                                                    <  1:389060/143‑1 (MQ=255)

                                                         tGG‑CACGAATCGTGTGTTTGTGAATTTGGCTCATGCTTTTGTGCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGaa                                                                                    <  1:293986/143‑1 (MQ=255)

                                                           g‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCGTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAAtt                                                                                  >  2:935494/1‑143 (MQ=255)

                                                           g‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCATTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGGAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAAtt                                                                                  >  1:313614/1‑143 (MQ=255)

                                                             cAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCCAAAGTAGAATtg                                                                                 >  2:300159/1‑143 (MQ=255)

                                                             cAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAGAGTAGAATtg                                                                                 >  1:593224/1‑143 (MQ=255)

                                                              aGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGGAGTTTTGTTTAGTATTTGGGCGAATGATCACGCAAAAGTAGGATtgt                                                                                >  1:876905/1‑143 (MQ=255)

                                                                gAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTCCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGc                                                                              <  1:429948/143‑1 (MQ=255)

                                                                 aaTGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGCATTGTGCa                                                                             >  2:326634/1‑143 (MQ=255)

                                                                 aaTGGTGTGTTTGTGAAGTTGGCGCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCa                                                                             <  1:108313/143‑1 (MQ=255)

                                                                 aaTGGTGTGTTTATGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCa                                                                             >  1:15549/1‑143 (MQ=255)

                                                                 aaTGGTGAGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCa                                                                             >  2:924660/1‑143 (MQ=255)

                                                                    tgtgtgtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGAGGTTAGTAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAAt                                                                          <  1:520274/142‑1 (MQ=255)

                                                                      tgtgtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTGGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATaa                                                                        >  1:636938/1‑143 (MQ=255)

                                                                       gttttgttgAATTTTGCTCATGCTTTTGTTCTGCATGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATaaa                                                                       <  2:646541/136‑1 (MQ=255)

                                                                            tgtgAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTGTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCa                                                                  >  2:17122/1‑143 (MQ=255)

                                                                             gtgAATTTGGCTCATGCTGTTGTTCTCCTTGTAGGATGTTAAGAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGGTTTGTTTGGTATTTGGCCGACAGATAACGCAAAAGTAGAATTGTGCAAATAACCGGCAg                                                                 >  2:493204/1‑143 (MQ=255)

                                                                              tgAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAgg                                                                <  1:855499/143‑1 (MQ=255)

                                                                              tgAATTTAGCTCATGCTTTTGTTCTCCGTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑CGG‑GGTATTGTCAGTTTTGTTTAGTATTCGGGCGACAGATCAAGCAAAAGTAGATTTGTGCAAATAAACGGCAgg                                                                <  2:515935/143‑1 (MQ=255)

                                                                                aaTTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTAGGTGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGt                                                              >  2:588049/1‑143 (MQ=255)

                                                                                aaTTTGGCTCATGCTGTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTATTGTTAAGTAGATGGGCGACAGATCACGCAAATGAAGAATTGTGTAAATAAACGGCAGGGt                                                              >  2:199562/1‑143 (MQ=255)

                                                                                    tGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtt                                                          <  1:43226/143‑1 (MQ=255)

                                                                                        tCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATACGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTg                                                      >  2:369308/1‑143 (MQ=255)

                                                                                          ctGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGT‑GGTATTGTCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGaa                                                    <  1:475249/142‑1 (MQ=255)

                                                                                          aTGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGaa                                                    >  1:309179/1‑143 (MQ=255)

                                                                                            gCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTCGAATTGTGCAAATAAACGGCAGGGTAATTTTTGagga                                                  >  2:335890/1‑140 (MQ=255)

                                                                                              ttttGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACTGATCACGCAAAAGTAGAATTGTGCAAATACACGGCAGGGTAATTTTTGAAGGTc                                                >  2:521117/1‑143 (MQ=255)

                                                                                              ttttGTTCTCCTTGTAGGATGTTAATAATATGAGGCATAAGCGTTAAAT‑G‑TGAGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTc                                                >  2:518660/1‑143 (MQ=255)

                                                                                               tttGTTCTCCTTGTAGGATGTTACTAATATCTGGCAGAAGCGGTAAAT‑GTT‑AGG‑GGTATTGGTAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCa                                               <  1:500355/143‑1 (MQ=255)

                                                                                                ttGTTCTGCTGGTCGGGTGTTAATAATCTGGTGCATGAGCGTTAAAT‑GTT‑ACG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAg                                              <  2:832027/143‑1 (MQ=255)

                                                                                                ttGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAg                                              <  1:54892/143‑1 (MQ=255)

                                                                                                 tGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGc                                             <  1:675520/143‑1 (MQ=255)

                                                                                                   ttCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGCCAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGcaa                                           >  1:319049/1‑143 (MQ=255)

                                                                                                   ttCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGcaa                                           >  2:539428/1‑143 (MQ=255)

                                                                                                   ttCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTGGATTAGTATTTGGGCGACAGATAACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATATTTGAAGGTTAGcaa                                           >  2:466987/1‑143 (MQ=255)

                                                                                                   ttCTCCGTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAG‑GTT‑AGG‑GGTAGTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGGAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGcaa                                           >  1:541508/1‑143 (MQ=255)

                                                                                                   ttCTACTAGAAGGATGATAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGAATTGGAAGTTTTGTTAAGTATATGAGCGACAGGTCACGCAAAAGTAGAATTGTAAAAATAAACGAGAGCGAAATTTTAGAAGATAAGcaa                                           >  2:76427/1‑143 (MQ=255)

                                                                                                      tcCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGcaacaa                                        >  1:565865/1‑143 (MQ=255)

                                                                                                       ccTTGTGGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACaaa                                       <  2:718938/143‑1 (MQ=255)

                                                                                                             aGGATGTTAAAGATGTGTGGAATACGCGTCAAAC‑GTT‑GGG‑GGTGTTGGCGGTTTTGTGTAGTATTTGGGCGACAGATCACGCAAAAGTAGGATCGTGCAACTAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGCTGa                                 <  2:109078/143‑1 (MQ=255)

                                                                                                               gATGTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAtt                               >  2:711438/1‑143 (MQ=255)

                                                                                                                 tGTTAATAATATGTGGCATAAGCGTTAAAG‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTaa                             <  2:659061/143‑1 (MQ=255)

                                                                                                                  gTTAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAAt                            >  1:191475/1‑143 (MQ=255)

                                                                                                                   ttAATAATATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAATTGAGTAAtt                           >  2:810562/1‑143 (MQ=255)

                                                                                                                     aataatATGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATCAATTCt                         >  2:211066/1‑143 (MQ=255)

                                                                                                                       taataTGTGGCATAATCGTTAAAT‑GTT‑AGG‑AGTATTGGGAGTTTTGTTTAGTATTTGAGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                       <  1:647750/143‑1 (MQ=255)

                                                                                                                       taataTGTGGCATAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAATGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                       <  2:77035/143‑1 (MQ=255)

                                                                                                                                 cgtgaGCGTTGAGT‑GTT‑AGT‑GGTATTGGCAGGTTTGTTTAGTATTTGGGCGACAGAGGACGCAGAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATg             <  2:14376/139‑1 (MQ=255)

                                                                                                                                 cATAAGCGTTAAAT‑GTT‑AGG‑GGTAGTGGCAGTTGTGTTTAGTATTTGGGCGAAAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATg             <  1:12112/143‑1 (MQ=255)

                                                                                                                                   tAAGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTGCGTTTATTATTTAGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCa           >  2:72612/1‑143 (MQ=255)

                                                                                                                                    aaGCGTTAGGT‑G‑TGAGG‑GCAACTGTCAGGTTTGGTTAGTATGTGGGCGAGAGATCACGCGAAAGTAGAATTGTGCAAATAAACGGCAGGGTTATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAg          <  2:5924/143‑1 (MQ=255)

                                                                                                                                    aaGCGTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAg          >  1:268750/1‑143 (MQ=255)

                                                                                                                                        gTTAAAT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTAGATTAATTCTTTGAGGAACATGCAGatat      >  2:874215/1‑143 (MQ=255)

                                                                                                                                          tAAAT‑GTT‑AGG‑GGTATTGGCGGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatgc    <  2:355870/143‑1 (MQ=255)

                                                                                                                                           aaaT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATCAACGGCAGGGTAATTTTTGCAGGTAAGCAACAAAAGTTGATTACTTCTTTGAGGAACATGCAGTTatgca   >  1:451964/1‑143 (MQ=255)

                                                                                                                                           aaaT‑GTT‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATCAATTCTTGGAGGAACATGCAGTTatgca   >  2:78115/1‑143 (MQ=255)

                                                                                                                                            gaggg‑T‑AGG‑GGTTGTGGCATTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGGAGAATTGTGCAAATAAACGGCATGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatgcat  <  2:908848/139‑1 (MQ=255)

ATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGG‑CAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAT‑G‑T‑AGG‑GGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCAT  >  NC_000913/4287281‑4287561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`