Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 16,923 T→G 9.7% K13T (AAG→ACG)  mokC ← regulatory protein MokC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091316,9230TG 9.7% 100.1 / 3.2 47K13T (AAG→ACG) mokCregulatory protein MokC
Reads supporting (aligned to +/- strand):  ref base T (18/22);  new base G (3/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

TCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCG  >  NC_000913/16789‑17057
                                                                                                                                      |                                                                                                                                      
tCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGCCCTTGCGGTcc                                                                                                                                >  2:373722/1‑142 (MQ=255)
tCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTGTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGGTGACGATCAGGGCGAGAATCCTCGCCTTATGCTGCTTCATTGCTCTCTTCTGCTTGGCCTTACGGTCa                                                                                                                                >  1:448855/1‑143 (MQ=255)
   ggCCAGTTCCGACGTGAACCTCCCAGGGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCGTAAGGTCaagg                                                                                                                             <  2:615715/143‑4 (MQ=37)
    gacggtacGGAGGTGAACCAGACAGAGGTCGGTTCTCGTTACCAGCGCCGCCACGACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTGCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTaa                                                                                                                            <  1:401165/136‑1 (MQ=37)
       gggtCGGGTGTGAGCCTCACAGAGGTCTCTTCTGGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACTATCATCGCCTTATGCTGCTTCATTGCTCTCTGCTCCTTGACCTTACGGTCAGTAagag                                                                                                                         <  2:58279/140‑1 (MQ=37)
       aGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAagag                                                                                                                         <  2:887097/143‑1 (MQ=255)
          ttgcgtgtgACCCTCCCCGCGGTCTTTTCCCGTTACCATCCCCGCCACTACGGCGCTGATACCGATGACGATCAGCGCTACAATCCTCGCCTTCTGCTGCTTCCTTGCTCTCTTCTCCTGGACCTTACGGTCAGTAAGAGGCa                                                                                                                      <  2:610465/138‑1 (MQ=255)
             gaggtcaaCCGCACAGAGGTCCTTGCACGGTACCACCGGCGCGCCTACGGTCGTGGTACAGATGACGATCAGGGCGACAATCATCGCCTTAGGCTGCTTCAGGGCTCTCTTCTCCTTGACCTTACGGTCCGTAAGAGGCGctc                                                                                                                   <  2:647197/137‑1 (MQ=255)
                gggAACCCCACCGAGGTCTTTTCTCGTTGCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTAc                                                                                                                <  2:604270/141‑1 (MQ=255)
                 tgAACCACACAGAGGTCTTTTCTCGTTACCAGCGCCGCAACTACGGCGGTGATACATATGACGATCAGGGCGACAATCATCGCCTTATGCGGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACa                                                                                                               <  1:88868/143‑1 (MQ=255)
                  gAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGCGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACAt                                                                                                              <  2:121953/143‑1 (MQ=255)
                           agagGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGc                                                                                                     >  1:923828/1‑143 (MQ=255)
                                  ttttCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACTATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCGCTCTTCTCCTTGACCTTACGGTCAGTAAGGGGCACTCTACACGTGTTCAGGAtatatg                                                                                              >  1:775336/1‑141 (MQ=255)
                                   tttCTCGTTACCGGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATagga                                                                                             <  2:558041/143‑1 (MQ=255)
                                              cAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGtt                                                                                  >  1:559313/1‑143 (MQ=255)
                                                  gccgccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATg                                                                              <  1:339305/143‑1 (MQ=255)
                                                  gccgccACCACGGCGGTGATACAGATGACGATCAGGGCGACAATCAGCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATg                                                                              <  1:351902/143‑1 (MQ=255)
                                                   ccgccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTATCTTCTCCTTGAACTTACGGTCAGAAAAAGGCACTCTCCATGTGTTAAGCTTATAGGAGGCCTCCGGTTGATgg                                                                             >  1:406895/1‑143 (MQ=255)
                                                                  tGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCGTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCgg                                                              <  2:667579/143‑1 (MQ=255)
                                                                  cgatattgATTACGATCAGGGCCGCAATCATCGCTTTATGCTGCTTCCTTGCACTCATCTATTTGAACTTACGGTGAGTAAGAGGCACTCTACATGTATTCAGCATATAGGTGGCCTCGGGTTGATGGTAAAATATCACTCgg                                                              <  2:251624/136‑1 (MQ=255)
                                                                      aCAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGCTGGTCAAATAGAACTCGGTGCt                                                          >  2:526948/1‑143 (MQ=39)
                                                                      aCAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCt                                                          >  2:11868/1‑143 (MQ=255)
                                                                         gATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCtttt                                                       <  1:390239/143‑1 (MQ=255)
                                                                            gACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTtctc                                                    <  2:730200/143‑1 (MQ=255)
                                                                                 tCAGTGTGATAATCATCGCCTTATGCTTCTGCATTGCTCTCTTCTCCCTGACCCAACGGTCAGTAAGAGGCACCCTACACGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCt                                               <  1:18788/143‑1 (MQ=255)
                                                                                     ggCGACAATCGTCGCCTTCTGCTGCTTCATTGCGCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCg                                           <  2:619720/143‑1 (MQ=255)
                                                                                     ggCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCg                                           <  2:344031/143‑1 (MQ=255)
                                                                                      gcgcCAATCATCGCCTTATGCTGCTTCATTGCTCTCTCCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGt                                          <  2:487812/143‑1 (MQ=255)
                                                                                       cGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGtt                                         >  1:276485/1‑143 (MQ=255)
                                                                                           cgccaccGCCTTATGCTGCTGCATTGCTCTCTTCTCCTGCACCGTACGGTCAGTAAGAGGCACTCTACATGTGGTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGc                                     <  2:338320/137‑1 (MQ=255)
                                                                                           aaTCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGc                                     >  1:808320/1‑143 (MQ=255)
                                                                                              catcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTaa                                  >  1:384238/1‑143 (MQ=255)
                                                                                                   ccTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCCGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCGGTTCAGCTaaaccat                             >  2:244983/1‑138 (MQ=255)
                                                                                                    cTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTCATGCCTg                            >  1:213566/1‑143 (MQ=255)
                                                                                                    cTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAGTGCCTg                            >  1:393585/1‑143 (MQ=255)
                                                                                                    cTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTg                            >  2:755182/1‑143 (MQ=255)
                                                                                                     ttATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCCTTCAGCTAATGCCtca                           >  1:800510/1‑141 (MQ=255)
                                                                                                       aTGCTGCTTCATTGCTCTCTTCTCCTTGGCCTTACGGTCAGTAAGAGGCCCTCTTGGTGTGGCCACCACATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTgaga                         <  1:980207/143‑1 (MQ=255)
                                                                                                            gctTCATTGCTCCCTTCTCCTTGACCTTCCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGagacagac                    <  1:99440/143‑1 (MQ=255)
                                                                                                              ttCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTGATGCCTGAGacagacag                  >  1:386458/1‑143 (MQ=255)
                                                                                                                  ttGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTc              <  1:11868/143‑1 (MQ=255)
                                                                                                                  tggCTCTGTACTCGTTGACCATACGGTCAGGAAGAGGCACGCTACATGTGTTCAGCATATAGGAGCCCTCGGGTTGAGGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTc              <  1:211437/141‑1 (MQ=255)
                                                                                                                         ctcctcCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCAcc       <  1:40539/140‑1 (MQ=255)
                                                                                                                          ttctCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCAccc      >  2:813514/1‑143 (MQ=255)
                                                                                                                          ctcttactGACCGTAAGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGGTGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCCAGCAccc      >  1:270829/8‑143 (MQ=255)
                                                                                                                              tactGACCGTAAGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACccgccg  >  2:482642/4‑143 (MQ=255)
                                                                                                                              tactGACCGTAAGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCGGAGACGGACAGCCTCAAGCACccgccg  >  1:125979/4‑143 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCG  >  NC_000913/16789‑17057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑