Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 887,468 G→A 7.4% A16T (GCC→ACC)  rcdA → DNA‑binding transcriptional regulator RcdA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913887,4680GA 7.4% 140.9 / 2.7 54A16T (GCC→ACC) rcdADNA‑binding transcriptional regulator RcdA
Reads supporting (aligned to +/- strand):  ref base G (31/19);  new base A (2/2);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

TCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGT  >  NC_000913/887336‑887608
                                                                                                                                     |                                                                                                                                            
tCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGa                                                                                                                                    <  1:387399/143‑1 (MQ=255)
   tAGATCACGTCGAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGGGAGAGGAAATGACGTGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGc                                                                                                                                 >  1:624269/1‑143 (MQ=255)
         gcGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGaa                                                                                                                           <  2:547135/142‑1 (MQ=255)
           gTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAAc                                                                                                                         >  2:892249/1‑143 (MQ=255)
            tCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCCGGAGGCGGTGAAACt                                                                                                                        >  2:388554/1‑143 (MQ=255)
                     gaagaGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAAt                                                                                                               >  1:666394/1‑143 (MQ=255)
                        gagagaAGACAACATTAGCAAGGATGTGAACATATGGTCACAATTGCAGTGAGAGTAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACa                                                                                                            <  1:668229/143‑1 (MQ=255)
                         agGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCACCGGCGAG‑AAAAAATGATCCAGGCCACGCGGGAGGCGGTGAAACTTTACGGAATACAt                                                                                                           >  1:278345/1‑143 (MQ=255)
                              aGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGACCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGt                                                                                                      >  1:27415/1‑143 (MQ=255)
                              aGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGACCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGt                                                                                                      >  1:105528/1‑143 (MQ=255)
                                acaacaTTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTa                                                                                                    <  1:690486/143‑1 (MQ=255)
                                  aacaTTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACg                                                                                                  >  1:632910/1‑143 (MQ=255)
                                  aacaTTAGCAACGTTGTGGACATTTGTACACAAGTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACg                                                                                                  <  2:111027/143‑1 (MQ=255)
                                       tAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCg                                                                                             <  1:892249/143‑1 (MQ=255)
                                               ttGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTg                                                                                     >  2:627721/1‑143 (MQ=255)
                                               ttGTGTACATTTGTACACAAGTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTg                                                                                     <  1:902642/143‑1 (MQ=255)
                                                       aTTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCtt                                                                             >  1:799509/1‑143 (MQ=255)
                                                       aTTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCtt                                                                             >  2:121203/1‑143 (MQ=255)
                                                       aTTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCtt                                                                             <  2:149659/143‑1 (MQ=255)
                                                              cacaATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTCACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCgggg                                                                      >  1:769366/1‑143 (MQ=255)
                                                               acaATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGATGGCCACATTATGTTGATTCGGAATACATGCTGTTACGCACCGCACAATTGCTACCCTTGCCGGGGt                                                                     >  1:719298/1‑143 (MQ=255)
                                                                 aaTTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCACCGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAGTACATGCTGTTACGCACCGCACAATTGCTACCCTTGCCGGGGTAc                                                                   >  1:561449/1‑143 (MQ=255)
                                                                 aaTTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCACAATTGCTACCCTTGCCGGGGTAc                                                                   >  2:512435/1‑143 (MQ=255)
                                                                     gCAGAGAGAGGAAATGACATTCGTCGCGCTAACGCTCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGtt                                                               <  1:646309/143‑1 (MQ=255)
                                                                       agagagagGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGATACGCACCGCAAAATTGCTACCCTTGCCGCGGTACCGGTgg                                                             >  2:3189/1‑143 (MQ=255)
                                                                         agagagGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCCCGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTCACGCACCGCAAAATTTCTACCCTTGCCGGGGTACCGTTGGGa                                                           >  2:389708/1‑143 (MQ=255)
                                                                           aggggAAATGACATGGTGCGCGCTAACGAGCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATc                                                         <  2:278345/140‑1 (MQ=255)
                                                                           agagGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGACGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGGTc                                                         >  1:217811/1‑143 (MQ=255)
                                                                           agagGAAATGACATGCGTCGCGCGAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATc                                                         <  2:532035/143‑1 (MQ=255)
                                                                             agGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCg                                                        >  1:862298/1‑143 (MQ=255)
                                                                               gAAATGACAGGCGGCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCAGTCACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGATGGGATCGATg                                                     >  2:753732/1‑143 (MQ=255)
                                                                                aaaTGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGACCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGa                                                    <  2:27415/143‑1 (MQ=255)
                                                                                aaaTGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGACCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGa                                                    <  2:105528/143‑1 (MQ=255)
                                                                                 accgACAAGCGGCGCGCTAACGATCGGCACCGGCGAG‑AAAAAAGTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGCAGACAGGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGAc                                                   <  2:898568/140‑1 (MQ=255)
                                                                                  aTGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTGCCCTTGCCGGGGTACCGTTGGGATCGATGAcc                                                  >  1:870848/1‑143 (MQ=255)
                                                                                  aTGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGAcc                                                  >  1:884598/1‑143 (MQ=255)
                                                                                   tGACATGCGCCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACct                                                 <  2:614702/143‑1 (MQ=255)
                                                                                    gACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCCCGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACcta                                                <  2:591773/143‑1 (MQ=255)
                                                                                       aTGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAGAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACctacta                                             >  1:135873/1‑143 (MQ=255)
                                                                                         gCGTCGCGCTAGCTATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCGCGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTAtt                                           <  2:745064/143‑1 (MQ=255)
                                                                                            tCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGACGACCTACTAttgtc                                        >  2:599984/1‑140 (MQ=255)
                                                                                              gcgcTACCGACCCGCAAGGGCGAG‑AAAAAAGTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCa                                      <  1:164418/143‑1 (MQ=255)
                                                                                              cagcTAACTATCCGCAACGACGAG‑AAAAAATTATCCAGGCCACGCTGGACGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCa                                      <  1:407125/141‑1 (MQ=255)
                                                                                               cgcTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAg                                     >  1:308101/1‑143 (MQ=255)
                                                                                                  tAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATCTTTCAGGaa                                  >  1:99955/1‑143 (MQ=255)
                                                                                                   aaCGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAAt                                 <  1:529118/143‑1 (MQ=255)
                                                                                                         ccGCAACGGCGAG‑AAAAAATTATCCCGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATtgatga                           <  1:583599/143‑1 (MQ=255)
                                                                                                                ggCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTCTTTTTCAGGACATGCTCAGTTGTTa                    >  1:736164/1‑143 (MQ=255)
                                                                                                                    ag‑aAAAAATTATCCAGGCCGCGCTTGTGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAGTTGTTACTgg                >  2:865093/1‑143 (MQ=255)
                                                                                                                     g‑aAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACGTGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCCGGAATTGATGAGTTGTTACTGGa               >  1:896898/1‑143 (MQ=255)
                                                                                                                     g‑aAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGGTCGATGACCTACTCTTTTTCAGGGATTGATGAGTTGTTACCGGa               >  2:25184/1‑143 (MQ=255)
                                                                                                                                tCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGGATTGATGAGTTGGTACGggcggccgtcggc     >  1:343954/1‑136 (MQ=255)
                                                                                                                                tCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAGTTGTTACTGGAGGCGTTcagc     >  1:549340/1‑143 (MQ=255)
                                                                                                                                   aGGCCACGCTGGAGGCGGTGAAACTTTACGGAATCCGTGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGt  >  1:511403/1‑143 (MQ=255)
                                                                                                                                     |                                                                                                                                            
TCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAG‑AAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCAGGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGT  >  NC_000913/887336‑887608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑