| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 915,080 | G→C | 6.9% | intergenic (‑223/+272) | lysO ← / ← aqpZ | L‑lysine exporter/water channel AqpZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 915,080 | 0 | G | C | 6.9% | 130.4 / 3.5 | 53 | intergenic (‑223/+272) | lysO/aqpZ | L‑lysine exporter/water channel AqpZ |
| Reads supporting (aligned to +/- strand): ref base G (25/24); new base C (2/2); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01 | |||||||||||
TTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCC > NC_000913/914938‑915219 | ttAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATg > 2:401901/1‑143 (MQ=255) aTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGTGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGCt < 2:853786/143‑1 (MQ=255) gTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGa < 1:72650/143‑1 (MQ=255) gTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCGTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGGGGGGTGa > 2:326187/1‑143 (MQ=255) tgtagCAAAAGGACGAGTATAGTATTCTGGTTCATTTAAAGATATTGTTGCGCTTTAATTACAAACTGCACCGATGTTTGTGGCGTCAAAATCGCCGAGGCGTTCACTGAAGGCCAGGGCAGCCCACATGGATGTGGGCTGAg < 2:333184/139‑1 (MQ=21) aTAAGGAGCAGTAGAGTATTCGTTTTGATTCAAAGAAATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGACGTCGATATCGCCGAGGCGTTCCCTGAAGGCCGGGGCGGCCCAGATGGAGGTGGGGTGAGGGcgcg > 2:485337/1‑143 (MQ=25) aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGcgcg > 2:181401/1‑143 (MQ=255) gAGCAGTAGAGTATTCGTTTTCATTTAAAGCTATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTa < 2:368021/143‑1 (MQ=255) tgTAGAGGATTCGTTTTCATTTAGAGATATTCTTGCGCTTTAATTACAAGCTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAgg < 2:780547/142‑1 (MQ=255) gTAGAGTATTCGTTTTCATGTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAggg < 2:488330/143‑1 (MQ=255) agagTATTCGTTTTCATTGAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAt < 2:688335/143‑1 (MQ=255) attATTCGTTTTCATTTAACGTTACTCCTGCGCTTTAACTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGt < 2:972269/141‑1 (MQ=18) tATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCGCATGGACGGGGGCTGAGGGCGCGGGTTACAGGGGTGttg > 1:73198/1‑142 (MQ=25) aTTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTAc > 2:284868/1‑143 (MQ=255) ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCCGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTAcc > 1:453459/1‑143 (MQ=255) ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTAcc < 2:377032/143‑1 (MQ=255) gTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTcg > 1:780526/1‑143 (MQ=255) cttgcATTGAAGGATATTCTTGCGCTCTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAAGCGCCGAGGCGTTCCCTCAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTcgc < 1:403675/139‑1 (MQ=18) ttCATTTAAAGATATGCTGGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCgcgc < 2:483253/143‑1 (MQ=255) aTTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCg < 2:949280/143‑1 (MQ=255) tttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGCt < 2:347117/106‑1 (MQ=255) tttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGCt > 1:347117/1‑106 (MQ=255) tAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGAcc < 2:631222/143‑1 (MQ=255) atatTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTa > 1:132568/1‑143 (MQ=255) tgaaTCACAACCGGCCCCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATa < 1:153830/141‑1 (MQ=255) tACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCg > 2:103373/1‑143 (MQ=255) aCAAACTGCACCGCCGTTGGTGGCGTCAAAACCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGa < 1:116324/143‑1 (MQ=255) cGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCgcgc > 2:146799/1‑143 (MQ=39) aTGTTTGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACCGGGCAGTGGGCTGAGGGCGCGTCTCACAGGGCTGTTACCTCCCGCCCGACCCGGTGGCCGTAAGGGGTGAGCCGAGGGCACCGCGCaa > 2:787642/1‑142 (MQ=11) ttGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcgg < 1:401901/143‑1 (MQ=35) ttGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcgg < 1:428782/143‑1 (MQ=35) gTCAAAGTCGCGGTGGCGGTCCCTGAAGGCCGGGGCAGCCGACCTGGATGTGGGCTGAGGGCGCGTTTTACAGGGCTGTTACCTCGCGCCCGACCCGGTAGCCGTATGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTg < 2:19219/143‑1 (MQ=11) gTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGTGGGCGCGTTATACAGGTATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGCTCAGTCGAGGGCACCGCGCAGCGGCGATTTTg > 2:961705/1‑143 (MQ=11) tCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGGTTTTGt > 2:125473/1‑143 (MQ=25) tCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGt > 1:338002/1‑143 (MQ=35) aaaaGCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTc < 1:422770/143‑1 (MQ=21) aaaTCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGATGAGGACTTGAGTCACGGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCg < 1:719535/143‑1 (MQ=11) gCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCaga < 2:881048/143‑1 (MQ=32) gCCGAGGCGTGCCCTGAGGGCGGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCaga < 2:148816/143‑1 (MQ=12) aGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTCTTCGCCAGAGccc > 1:712382/1‑143 (MQ=17) cccTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTg > 1:928255/1‑143 (MQ=32) ggaggggggggCAGCCGACATGGATGGGGGCTGAGGGGGTGTTTTACAGGGATGTTACCTCGGGCCCGACCCGGTAGCCGTAAGGGATAAGTGGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAgg < 2:472213/136‑1 (MQ=11) gAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAgg > 2:866029/1‑143 (MQ=32) gAAGGCCGGAGCAGCCCACATGGATGTAGGCTGAGGGCGCGTTTTACAGGGATGGTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATCAGTCGAGGGCGCCGCGCAGCGGCGACGTTGTTCGCGAGAGGCCGGGGGAGCAgg > 2:360014/1‑143 (MQ=11) gcggggggCAGCCGAGAGGGGTGTGGGCTGAGGGGGCGTTTGAGAGGGATGTTACCGCGCGCCCGACCCGGTAGCGGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAggggg < 1:866091/139‑1 (MQ=11) ccGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTCTGTTCGCCCGAGCCCGGGGGTGCAGGGggcg > 1:822473/1‑143 (MQ=17) gggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggcg > 1:284340/1‑143 (MQ=32) gggCAGCCCACATGGATGTGGGCTGAGAGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAGGTCGAGGGCACGGCGCAGCGGCGATTTTGTTCGCCAGAGCCGGGGGGTGCAGGAggcggag > 2:84844/1‑141 (MQ=11) gggCAGCCCACATCGATGTGGCCTGAGAGCGCGTATTACAGGGACGTTACCTCGCGCGCGCCGCGGTAGCCGTGAGGGGCAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGAGCGAGGagcggag > 2:215654/1‑141 (MQ=11) gCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggcggc > 1:844057/1‑143 (MQ=32) gCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTGACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggcggc < 1:181401/143‑1 (MQ=17) cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCAGGAGCCCGGGGGTGCAGGGGGCGGCGGCGAtg > 1:263628/1‑142 (MQ=17) cccACATGGATGTGGGCTGAGCGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCGCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAtt > 1:197807/1‑143 (MQ=17) caTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgcc > 1:12186/1‑143 (MQ=32) | TTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCC > NC_000913/914938‑915219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |