| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,196,247 | A→G | 100% | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,196,247 | 0 | A | G | 100.0% | 62.7 / ‑6.7 | 28 | L375M (TTA→CTG) | icd | isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (11/16); total (12/16) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGT > NC_000913/1196111‑1196307 | aCGAATGGGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCTTGGTTCTATTATTAGCTCCGCTGAGATGATGCGGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGtt < 1:914962/143‑1 (MQ=37)aCGAATGCGCCCTGGTTGAAGCCACCCACGGTACTGCGCAGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGAGCAAAATTGATCGGACTGAAGCGGCTGACCTGATTGtt < 2:808939/143‑1 (MQ=255) aaTTCGCCCTGTTTGAAGCCACCCACCGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa < 1:67368/143‑1 (MQ=37) aTGGGCCCTGTTTGAAGCCTCCCGCGGTACTGCGCCGAAATGTGCCTGTCAGGACAAAGTAAATCCTGGCTCTATTCTTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg < 2:825595/143‑1 (MQ=25) aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg < 2:772232/143‑1 (MQ=38) ggcgcTGTTTGAATCCAACCACGGTCCTGCGCCGAAATATGCCGGGCAGGACAAAGTAAATCCTGGCTCTATGATTCTCTCCGCTGGGATGATGCTGCGCCATATGGGGTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa < 2:642234/139‑1 (MQ=25) cgcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAACTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATGGTTAAGGGTa > 2:200812/1‑143 (MQ=37) gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt > 1:985933/1‑143 (MQ=255) cccTGTTTGGAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATg < 2:496053/143‑1 (MQ=37) cTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa < 1:122943/143‑1 (MQ=37) tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg > 1:626948/1‑143 (MQ=37) tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTCAAGCGGCTGACCTAAATGTTAATGGTTTGGAAgg > 2:383212/1‑143 (MQ=38) ttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAGGGGGTGGACGgc > 1:118950/1‑143 (MQ=37) ccaaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg < 1:244901/141‑1 (MQ=25) ccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg > 2:154918/1‑141 (MQ=25) cgcGGTGCGGCGGCGGAATAGGCCGGTCAGGACAGAGGAAATCCTGGCTGGATTGTTCGCTCCGCGGAGATGATGCTGCGCCAGATGGGTTGGACGGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGAAATCAAtgc < 2:658312/141‑1 (MQ=12) cacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc < 1:619753/143‑1 (MQ=21) cacGGCACGTCGCCGAAATATGCCGGTGAGGACTAAGTAAATCCTGGCTCTATTATCCTTTCCGCTGAGATCATGCTGCGCCATAGGGGTTGGACGGAAGTGGCTGACCTGATTGTGAAAGGTATGGAAGGCGCAATCAAtgc < 2:758593/143‑1 (MQ=18) acGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCGATATTGGTTAGACTGAAGCGGCTGACCTGATTGTTCAGCGGATGCAAGGCGCAATCAAtgcc > 2:345570/1‑142 (MQ=25) acGGTACTGCGCCAAAATATGCCGGTCAGGAAAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCAGCGCAATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:75676/143‑2 (MQ=21) ggTACTGCGCTGAGAGATGCCGGTCAGGACGAAGGAAAGCCGGGCGCTATTAGTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa < 2:202235/143‑1 (MQ=17) ggTAATGCGCCGGAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa < 2:841243/143‑1 (MQ=18) tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGCTAAAGGTATGGCAGGCGCACTCAATGCCAAGACCg > 2:427990/1‑143 (MQ=18) ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCCCCGCCGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc > 1:185087/1‑143 (MQ=17) cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct > 1:47835/1‑142 (MQ=18) gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt < 2:722500/143‑1 (MQ=17) ggTCAGGACAAAGTAAATCCTGACTCTATTATTCTGTGAGTGGAGAAGATGCTGCGGCTGATGGGTTGGACTGAAGCCGGTGACCTGGTTGTTAAAGGTATGGGAGGAGCGATCAATGCCAAGATCGTAACGTTTTACTTTGa > 2:964241/1‑143 (MQ=255) aGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTCACCTATGACTTCGAACGt > 1:945998/1‑143 (MQ=14) | ACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGT > NC_000913/1196111‑1196307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |