| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0% | 124.6 / ‑6.7 | 45 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (23/20); total (25/20) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132650‑2132926 | tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc > 1:252549/1‑143 (MQ=255) cTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCg > 1:612216/1‑143 (MQ=255) cTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCCGCATGTCACCGTGATTGATATGCAGGCTCTGCg > 2:717476/1‑143 (MQ=255) cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTcc > 1:349783/1‑143 (MQ=255) tatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg > 1:536995/1‑143 (MQ=255) tatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCCTCTACTTTCCACgg > 2:566737/1‑143 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt < 1:511153/143‑1 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGATAGAGGTAAAAGTGGAAGTACCCGGAGAATTTTTTAGACCGCAAGATACGCTGTATTTCACCGTGATTGATATGCAGGATCTGCGTAGAGTCGTCTCCTTTGTACGGTTGGcctggt > 2:117799/1‑139 (MQ=255) gcgGTCGAATGCGGCGTTGGGCTTGCGGTAATAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca < 2:465186/143‑1 (MQ=255) gccGAAGTTGTCTGTGTGCGTGCGGGAAAAGGGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca < 1:858515/141‑1 (MQ=255) gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGCGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTACTccacca > 2:369726/1‑143 (MQ=255) cGAAGTTATCTGTGTGCTTGCGGTAAAAGTGGGAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 2:59442/143‑1 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcccccacca > 1:191296/1‑143 (MQ=255) ttGTCTTGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCg < 1:892065/143‑1 (MQ=255) ggctgtgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa < 1:418173/138‑1 (MQ=255) tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAg > 2:458510/1‑143 (MQ=255) ccTTTTTGCGTGCGGTACAAGTGGAGGTCCCCGGAGCATTGTTTCGGTTGCGAGGTGATCTGCATTTCACCGTGATTGATATACAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg < 2:629731/142‑1 (MQ=255) ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa > 1:706488/1‑143 (MQ=255) gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAgcgcca > 1:570489/1‑138 (MQ=255) cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTCCTACTCTCCACGGTTCGCCGTCCTCCACCCGCCTCCGCAGGAAGATCTCGTa > 2:63715/1‑143 (MQ=255) aaaGTGGTCGTACCCGTTTAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt < 1:550735/143‑1 (MQ=255) ggAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGcc > 2:666668/1‑143 (MQ=255) ccgggaggaTTTTTGCCGTGACGAGGTGAGGTGGCTTTCACGGTGATTGAGATGCAGGCTCTGCGCCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTTCa < 2:355531/140‑1 (MQ=255) ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCCGGCAGCTGTCCTAATTCTGTGCCGCTTGCac > 1:397664/1‑142 (MQ=255) cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc > 2:428758/1‑107 (MQ=255) cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc < 1:428758/107‑1 (MQ=255) agaATTTTTTCGGTGACGAGGTGACCAGCATTTGACCGTTGTTGAGATGCAGGCTCTACGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:668712/143‑1 (MQ=255) ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGGTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTCCGCAGGAAGATGCGGTATGCCCGTCCCGCCTTCAGttagtcag > 2:883400/1‑137 (MQ=255) tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCTTCGCCTCTTCTACTTTCCACGGTTTCCAGTACTCCACCCCCATCCGCCGCAAGATGTCGTTATCCCTCGCCGCCTTCAGTTCggctcc > 2:448814/1‑139 (MQ=255) tggACGTGGTGTTCTGCATTTGACCGTGTCTCGTATGCATGCTCTGCGGCGCCTCTTCTACTTTCCACTGTTCGCCTGGCTTCACCCGCATCTGCAGGAAGATGTCGTAACCCTGTGCCGCGTTCAGTTCGGTCTCGaacaaa < 2:837622/141‑1 (MQ=255) tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTAGTAATCCGCGGCCGCTTTCACTTCGGTGTCGaacaaac > 2:338839/1‑143 (MQ=255) gACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGCAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca < 1:941321/143‑1 (MQ=255) ggTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt < 1:733975/143‑1 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTgga > 1:120247/1‑141 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCTTTCAGTTCGGGATCGAACAACCACTCTTTGacagg > 2:347878/1‑139 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg > 1:424386/1‑143 (MQ=255) aTTTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTTATCCTGTGCCGCTTTCAGCTCGGTATCGAACAAACACTCTTTGAAACGCCa < 2:518804/143‑1 (MQ=255) tCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGGGGCGGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc < 2:291263/143‑1 (MQ=255) attgatATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACGCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGCATCGAACAAACACCCTGCGAAACGCCACGCCCCGGTaa > 2:43700/1‑143 (MQ=255) tgtatGCAGCCTCTCCGTCTCGGCGTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTCGAACGCCATGCCCAGGTAAAGa < 2:986441/141‑1 (MQ=255) gatATGCAGGCCCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa < 2:612216/143‑1 (MQ=255) atctGCAGCCTCTGCGTCGCCTCTTGTACTTTCCACGGTTCTCCCTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTTTGCCGCTTTCAGTTCGGTATCCAACAAACACTCTTTGAAACGCCATGCCCATGTAAAGAc < 2:852832/140‑1 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt > 2:235357/1‑143 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCACAGGTAAAGACCTAGt > 1:326142/1‑143 (MQ=255) aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCTGTCTCCACCACCAACCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt < 1:390707/143‑1 (MQ=255) | TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132650‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |