| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,489,152 | C→T | 100% | S6N (AGC→AAC) | yfdE ← | acetyl‑CoA:oxalate CoA‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,489,152 | 0 | C | T | 100.0% | 118.2 / NA | 40 | S6N (AGC→AAC) | yfdE | acetyl‑CoA:oxalate CoA‑transferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/20); total (20/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGAC > NC_000913/2489016‑2489289 | tcgtgTATCATCACCATGACCCGGCGGCTCAACTTTAATTACACTTGCGCCTATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCat < 2:535421/139‑1 (MQ=255) tcatcaCCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGt < 2:135993/143‑1 (MQ=255) tgaCCAGGACCCGGCGACTCAACTTTAATTCCCAGTGCCACCAGATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAACATGACGAACGGCCCTTTGTTTTCATTATTTGTCAt < 2:545329/141‑1 (MQ=255) tcaCCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTAAGATCGGAAAAGACCATTAAGGACATGTGTCATATCGATGAATAAGAAGCCTTCAAACGACCAGTTGTGTGCATTATTGGTCag > 1:138085/1‑142 (MQ=255) tGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCTTTCAAACGGCTCTTTGTTTTCATTATTTGTCATTTGCtt > 2:238546/1‑143 (MQ=255) ccGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGc > 1:857963/1‑143 (MQ=255) ccGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACAAGTAAAATAAGTATTACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATga > 1:456630/1‑142 (MQ=255) gcggcTCAACTCTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCtt < 1:235197/143‑1 (MQ=255) ggcTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGa < 1:155171/143‑1 (MQ=255) aagtttAATTACCCTGGCGCCCATATTACAAAGAAGTTGAGTGCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGaaaaaa < 2:281980/140‑1 (MQ=255) aCTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAg < 1:527554/143‑1 (MQ=255) aCTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAg < 1:655618/143‑1 (MQ=255) cTTTAATTACCCGTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGc < 1:97187/143‑1 (MQ=255) tttAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTAATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATCCCTTGAAAAAAGcc > 2:603164/1‑143 (MQ=255) ttACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAgg < 1:531462/143‑1 (MQ=255) ttACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTAAAACGGACCTTTGTTTTCATTATTTGTCATTTGCTTATGACTTGAACACAGACGAAgg > 2:789657/1‑143 (MQ=255) ttGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAGGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGaaaa < 2:362720/143‑1 (MQ=255) ttGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGTCTTGAAAAAAGCCGAAGGTGaaaa < 1:346896/143‑1 (MQ=255) ttGCGACCAGATTACAAAGAAGTTGAGGTCCGAAAGGTGCATTAAGGACATGGGTCATATCGATAACTAATAAGCCTGCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGaaaa < 1:332656/143‑1 (MQ=255) aaaGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCAttt > 1:378883/1‑143 (MQ=255) aagaagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAACGACACTTCGGCAtata > 2:380191/1‑143 (MQ=255) aagTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAACAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGt > 2:878409/1‑143 (MQ=255) gAGTTCCGAAAGGTCCATTAAGGACATGTGTCATACCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAAt < 1:537136/143‑1 (MQ=255) tcgcaaGGTCCGTTAAGGACATGTGTCATATCGATAACTAATGAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATa < 2:450329/139‑1 (MQ=255) cGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATACCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTCAAATCAATaa > 1:350605/1‑143 (MQ=255) gTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGat > 1:663328/1‑143 (MQ=255) ggACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTACTTATGCCTTGAAAAAAGTCGAAGGTGAAAAGACACTTAGACATTTATGTAAAATCAATAAAATGATTAATCATGa > 2:191097/1‑143 (MQ=255) gACATATGTCTTTTTGTTAAGTATTAAGCCTTCAATCGGCCCTTTGTTTTCATTAGTTGGCATTTTCTTTTGCCTTATAAAAAGTCTAAGGTGAAAATGGGCTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGaa < 2:164742/143‑1 (MQ=255) gtCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTc < 1:378266/143‑1 (MQ=255) atatCGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGCAACCAGTCGAc > 2:936817/1‑143 (MQ=255) atatCGATAACTAATAAGCCTTCAAACGGACCTTTGTTTTCATTATTTGTCATTTGCTGATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATGAATTAAGAAACCAGTCGAc > 2:583631/1‑143 (MQ=255) cGATAACTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGAc < 1:802498/143‑1 (MQ=255) gATAACTAATAAGCCTTCAAACGGCCCGTTGTTTTCATTATTTGTCATTTGGTTATGCTTTGAAACAAGCCGAAGGTGAAGAGACACTTCGGCTTTTAAGTAAAATCAAAAAAATGATTAAGTATGAAACCCGTCGAACGACa > 2:492609/1‑143 (MQ=255) cTAATCAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGTTGAAAAGACACTTCGGCATTTATGTAAAATCAATAAAATGATTAATTATGAAACCAGTCGACTGAccaaaac > 2:818101/1‑137 (MQ=255) cTAATAAGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAAt < 1:238546/143‑1 (MQ=255) taataaGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATc > 1:812434/1‑143 (MQ=255) taataaGCCTTCAAACGGCCCTTTGTTTTCATTATTTGTCATTTGCTTATGCCTTCAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATCAATTATGAAACCTGTCGACAGACAAAAATc > 2:787290/1‑143 (MQ=255) ataaGCCTTCAAACGGCAGTTTGTTTTCATTATTTGTAATTTGCTTATGCCTTGAAAAAAGCAGAAGGTGAAAAGACACTTAGGAATTTATGTAAAATAAATAACATGATTAATTATGAAAACAGTTGAATGaaataaataaa > 2:362709/1‑140 (MQ=255) tcatccTTCCAACGGCCCTTGTTTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCcac < 2:874089/139‑1 (MQ=255) cTTTGTTTTCATAATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGCAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAGATCCACAAGGGAGCCGTgacgac > 2:625180/1‑143 (MQ=255) | GCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGAC > NC_000913/2489016‑2489289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |