| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,793 | C→A | 100% | L113F (TTG→TTT) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,793 | 0 | C | A | 99.9% | 122.4 / ‑7.5 | 41 | L113F (TTG→TTT) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (19/21); minor base G (0/1); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATC > NC_000913/2661653‑2661924 | aaCTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGCGATACGGTCGCTaaa > 2:233395/1‑143 (MQ=255)aaCTTAACGGCGAGCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTaaa < 1:348238/143‑1 (MQ=255) tCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAGAACCGGTGAGACGGTCGATAAAATCACGccg > 1:72498/1‑142 (MQ=255) cgTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGcgcg > 1:212916/1‑143 (MQ=255) ttGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGATGAGCCGGGGGCTAAAATCATGCCACATCGCGTggg > 2:849302/1‑143 (MQ=255) tgtgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCa < 1:781974/143‑1 (MQ=255) tgggCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCa < 1:751624/140‑1 (MQ=255) tgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCCCGTGGGTCAgg > 2:913774/1‑143 (MQ=255) gCGGGTGCGTCGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGc < 1:163010/143‑1 (MQ=255) cGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTGGCTAAAATCACGCCACAGCGCATGGGTGAGGCa > 1:788132/1‑143 (MQ=255) tGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTGGCTAAAATCACGCCACAGCGCGTGGGTCAGGCGTTTa > 1:538301/1‑143 (MQ=255) gcctGGCGCGTCGTGAGTATGCTGCCGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTAt < 2:266536/140‑1 (MQ=255) gCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTAt < 2:402560/143‑1 (MQ=255) tGAGTATGCTGACGACCAGACACATCCAGCACTTGCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGGGAAAACCGGTGAGACGGTCGCTAAAATCACGGCACAGAGCGTGGGTCAGGCAGTTATcgacgcaccggga > 2:46271/1‑136 (MQ=255) tGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGAGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCACTTATCGCCGCCCTGGCa > 2:978318/1‑143 (MQ=255) gAGTATGCTGACGGCCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAg < 2:43751/143‑1 (MQ=255) caGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAg < 1:592085/142‑1 (MQ=255) tGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAgccgcc > 1:624207/1‑143 (MQ=255) gCTGACGACCAGACACATCCAGCACTTGCTGGTTATTAACCGGTTCGCGTAAAGTAATGTTGTTGAGAAGACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCt < 2:109626/143‑1 (MQ=255) cgacCAGACACATCCAGCACATCGAAGTTGTCAACCAGTTCGCTTAAAGTAGTGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTAcc < 1:134401/143‑1 (MQ=255) agaTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACg < 1:962427/141‑1 (MQ=255) ccAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTACAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg < 2:724027/143‑1 (MQ=255) ccAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg < 1:480126/143‑1 (MQ=255) cACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCAt > 2:982671/1‑143 (MQ=255) aCTTCGTCGTTTTTAGCCAGTTCGCCTCCAGTAATGTTGTTGAGACAACCGGTTAGACGGTCGCTAAAATCCCGCCACAGCGCGTTGGTCAGGCATGTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATc < 2:137762/143‑1 (MQ=255) ttCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTa > 2:867825/1‑143 (MQ=255) tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCGTTACCCTGACAACGGGTGGCCTCTACAGa > 2:588763/1‑143 (MQ=255) ttccccAGTCCGCCTCACGTACGGGTGTTGAGAGCGCCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTCTCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc < 2:757319/139‑1 (MQ=255) tgaaCCAGGTCGCGTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGATCAAGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc < 1:621530/141‑1 (MQ=255) aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCaa > 1:162175/1‑143 (MQ=255) tcgaGTTCTCCTAAAGTAATGTTGTTGAGAAGACCGGTGGGACGGTGGCTGGTGTCGCCCCACATCCCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAAc < 1:719240/140‑1 (MQ=255) ccAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCCGCCGCCTTTACCCTGACAACGTGTGGCATCTCCAGCTTTGTccgcg > 1:293837/1‑139 (MQ=255) ggtCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCACCCACTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGgcg < 2:479564/141‑1 (MQ=255) gCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTaa < 1:428720/143‑1 (MQ=255) gCCTAAAGTAAGGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTaa < 1:474166/143‑1 (MQ=255) tAAAGTAATGTTGTTGAGAAACCCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACACTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTa < 1:233395/143‑1 (MQ=255) aGTAATGTTGTTGAGAAAACCGGTGAAACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTCTACCCTGACAATGGGTGGCACCTACAGACTCGTCAACGGCGCTACTTAcct > 2:940797/1‑141 (MQ=255) gttgttGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCaa < 1:573350/143‑1 (MQ=255) gttgAGAAAACCGGGGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACgg < 1:6992/143‑1 (MQ=255) ttgAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTGGCCAACGGc > 1:534744/1‑143 (MQ=255) agaAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCCAt > 2:550414/1‑143 (MQ=255) gagaCGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCANTTATCGCCGCCCTGGCAGCCGCCTTTNCCCTGACAACGGGGGGCATCTACAGATTCGTCAACGGCGCTAATTCCTTCGCCAACGGCGGTGCTGCTGGCAt > 1:896715/1‑143 (MQ=255) agaCGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATc > 1:497914/1‑143 (MQ=255) | AACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATC > NC_000913/2661653‑2661924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |