| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,398 | T→G | 100% | L29R (CTT→CGT) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,398 | 0 | T | G | 100.0% | 116.8 / ‑6.7 | 41 | L29R (CTT→CGT) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (23/17); minor base C (1/0); total (24/17) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCT > NC_000913/3041261‑3041536 | gCTATTTTACCATTAGTCATTAATACGCTGTTAAGCAACTCAGGGCTCTATTATGGCTTTCACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGcca < 1:537886/143‑1 (MQ=255) ttttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGCGccaccac > 2:708241/1‑142 (MQ=255) tttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTCCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAtt < 1:301887/143‑1 (MQ=255) cATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTgg > 1:680866/1‑143 (MQ=255) tAGCCATTAGTACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTCACACCTTTTCCTCCCCGTCAGCCTTCGGCTTCTGCCCGTTTACCGCTGCCGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcgc < 2:143933/143‑1 (MQ=255) ggTCGTTAAGACGCCGTTAGGCAACTCAGGGCTCTAGTATGGCTTTTACGCCTTTTCCTCCCCGTCAGCCTAGGGCGTCTGCCCGTGTGCCGCTGACGCTGATGACGCTTGGTGACTGGGCGCGTGCCACCATTACTGGcgcg < 2:863895/142‑1 (MQ=255) aGTCATTCATACGCCGTTAAGCAACTCCGTACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGcgcg < 2:898384/143‑1 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGCTGACGCTTGATGACTGGGCGCGTGCCCCCCCTCCTGGcgcgta > 1:591194/1‑141 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa < 1:186524/143‑1 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa > 1:47173/1‑143 (MQ=255) ttAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGGTGACTGGGCGCGTGCCGCCAGTACTGGCGCGGagag > 1:516099/1‑143 (MQ=255) aTACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCCTGCCCCCATTACTGGCGCGGACatcca > 2:119115/1‑139 (MQ=255) gTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTCGCCGCTGACGCTGAAGACAATGCTTGACTGGGCGCGTGCCACCATTACTGGCGACGCCAGCGaccaacac > 2:698878/1‑136 (MQ=255) aGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCCCCATTACTTGCGCCGACAGCCTAAAatataaaa > 1:153131/1‑140 (MQ=255) ggACTCTATTATGGCTTTTACACCGTTTGCGCCCCGACAGCCGACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg < 1:500429/143‑1 (MQ=255) ctctATTATGGCTTTTCCACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGa < 2:731922/143‑1 (MQ=255) tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGGGAcag > 2:409495/1‑143 (MQ=255) ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa > 1:778706/1‑143 (MQ=255) ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCCCTGCCGCTGATGACGCTTGATGACTGGGCGCGTCCCACCATTACTGGCGCCGCAAGCGCAAACTCTCCGCAGGGCCCGGTGCCAGCCGACGTcc > 1:208521/1‑142 (MQ=255) cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGACAGGTGACAGCAGCTGGCCGCCAGagg > 2:762036/1‑141 (MQ=255) ctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa > 1:709036/1‑143 (MQ=255) tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATCGCagaag > 1:345201/1‑143 (MQ=255) aGCCTGCGGCTGCTGCGCGCGCACGGCTGGCGCTGATGACGCTTGAGGACTCGGCGCGTGCCACCACTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCCACCAGATGGCAGAAGATCAGCAc < 2:602243/143‑1 (MQ=255) aCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAAGATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAGGGCAGGAGATGAGCACGTGCt > 1:743609/1‑143 (MQ=255) ttCTGCCCGTCTAGCGCTGATGCGGAGGACGCTCGATGACTGGGCGCGTGCGACCATAACTGGCGCGGACAGCGCAAAATATATGCAGGGTCAGGTGACAGCCGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccg < 2:589253/143‑1 (MQ=255) tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAACATATATGCAGGGTCAGGTGACAGCAGATGTCAGCGAGATGGCAGAAGCTCAGCACCTGCTggccggcc > 1:210249/1‑143 (MQ=255) ttACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAACAGATGTCAGCCAAATGGCAGAAGAACAGCACCTGCTCGCCACCCATTGcaa > 2:679752/1‑141 (MQ=255) ccGCTGACGCTGATGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGc < 2:282891/143‑1 (MQ=255) tgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaaa < 1:106345/143‑1 (MQ=255) tgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaaa < 1:790574/143‑1 (MQ=255) tgacgctgaGGACGCTGGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaaa < 1:609498/143‑1 (MQ=255) gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTa > 1:33888/1‑143 (MQ=255) ctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCGAAGGTaa > 1:249515/1‑143 (MQ=255) tgatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAGAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCGCCTGCTCGCCGCCCCTTCCGACGCCCGACGTaaa > 1:277026/1‑143 (MQ=255) tgatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCCGGGTCAGGAGACAGCAGATGTCAGCCCGATGGCAGAAGATCAGCCCCTGCTCGCCGCCCATTGCGCCGCCAGAGGTacg > 1:273227/1‑141 (MQ=255) aCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaaaaactg > 1:359729/1‑139 (MQ=255) tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgcc < 2:365308/102‑1 (MQ=255) tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgcc > 1:365308/1‑102 (MQ=255) tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTCCGGTGTGGCGCAATTTaag > 1:130297/1‑141 (MQ=255) gggCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGt < 2:778706/143‑1 (MQ=255) gcgcGTGCCACCAGGACTGGCCCGGACAGCGAAACATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCt < 2:467623/143‑1 (MQ=255) | GCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCT > NC_000913/3041261‑3041536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |