BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,335,655	T→A	5.0%	intergenic (‑65/+39)	eptA ← / ← adiC	phosphoethanolamine transferase EptA/arginine:agmatine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,335,655	0	T	A	5.0%	363.7 / 8.9	136	intergenic (‑65/+39)	eptA/adiC	phosphoethanolamine transferase EptA/arginine:agmatine antiporter
Reads supporting (aligned to +/- strand): ref base T (76/51); new base A (5/2); total (81/55)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-02

Only 100 of 137 total aligned reads displayed.

AGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAC  >  NC_000913/4335518‑4335796

agaTATAAAAAGCGGCCAACAATAGCCAGGCGGGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCGTCAACATCACGGTGTTTCCATCGAACAACGTGCGCATATGCTAGCAAAGGACAATTACGCCCACCTGAAGTTCTTaa                                                                                                                                          >  2:599227/1‑143 (MQ=255)

agaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTaa                                                                                                                                          >  1:826344/1‑143 (MQ=255)

   tataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCCCGCAACGCAACATTAAGCCAACCTTAAGTTCTTAAggg                                                                                                                                       >  2:889518/1‑142 (MQ=255)

    ataAAAGGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGtt                                                                                                                                      <  2:375374/143‑1 (MQ=255)

    ataAAAATCTGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGTGTGTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCTAACCTTAAGTTCTTAAGGtt                                                                                                                                      <  2:470571/143‑1 (MQ=255)

      aacaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTgg                                                                                                                                    <  2:175305/140‑1 (MQ=255)

            tggCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTa                                                                                                                              <  2:480544/142‑1 (MQ=255)

            cGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACCAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTa                                                                                                                              <  1:140386/143‑1 (MQ=255)

             ggCCAACAATAGCTAGTCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCATCAACATCACGATGTTTCCATCGAACAAAGTGCGCATATGCTAGCAAAGCAAAATTAAGCAAACATTAAATTATTAAGTTAGGCTTTTAt                                                                                                                             >  2:174572/1‑143 (MQ=255)

             ggCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGACCAAAGTGCGCATATGCTCGCAAAGCAAACTTAAGCAAGCCTTGATTTCTTAAGGTTCGCATTTAt                                                                                                                             >  1:763489/1‑143 (MQ=255)

             ggCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAGGTTCTTACGGTTGGCTTTTAt                                                                                                                             >  1:413328/1‑143 (MQ=255)

             ggCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATAGGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCATAAGGTTGGCTTatat                                                                                                                             >  2:174575/1‑143 (MQ=255)

             cgCCAACAACACCCAGGCGAGTAAATTCAAAGATGGACTGTGAAGCAAGCGCATCAACATCACGAAAAGACCAACGAACAAAGTGCGCATACCCAACCAAAGCAAAACTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTAt                                                                                                                             <  2:963847/142‑1 (MQ=255)

              gacaacaaGAACCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCGTTGATg                                                                                                                            >  1:725838/3‑143 (MQ=255)

               ccAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGATTCCATCGTACAAAGAACGTGTTGACGCGCAAAGCAAAATTAAGCCAACCTTAAGGTCTTACGGTTCGCtttcacgt                                                                                                                           >  2:345542/1‑138 (MQ=255)

                ctacaaTAGCCAGGCGAGTAAATTCAAAGAGGGGCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCGATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACATTAAGTTCTTCAGGTCGGCACTAATGtt                                                                                                                          >  2:364805/3‑143 (MQ=255)

                 aacaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAAGCTTCAGTTCTTAAGGTTGGCTTTCATGttg                                                                                                                         >  1:313570/1‑142 (MQ=255)

                        gCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGACCAAAGTGCGCATATGCTCGCACAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGAt                                                                                                                  >  1:127942/1‑143 (MQ=255)

                         ccAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTAGGTTTGCTGGAtt                                                                                                                 >  1:526857/1‑143 (MQ=255)

                           aGGCGAGTAAATTCCCAGAGGGTCTTTTTAGTCGGCGCTTCAACATCACGGTGTTTCAATCGAAGAAACTGCTCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTaa                                                                                                               <  2:64760/143‑1 (MQ=255)

                            ggCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAg                                                                                                              <  1:569483/143‑1 (MQ=255)

                             gCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAgg                                                                                                             <  2:968334/143‑1 (MQ=255)

                              taaGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACTGTGTTTTCATCGAACAAAGTGCGTATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAggg                                                                                                            <  2:629065/141‑1 (MQ=255)

                              cGAGTCAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAggg                                                                                                            <  2:812379/143‑1 (MQ=255)

                               gAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTGATGTTTGCTGGATTAAgggg                                                                                                           >  1:78855/1‑143 (MQ=255)

                                  tAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTACGTTCTTAAGGATGGCTTTTATGATCGCTGGATGAAGGGGAAt                                                                                                        >  2:571164/1‑143 (MQ=255)

                                  tAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAAt                                                                                                        >  1:490090/1‑143 (MQ=255)

                                    aaTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTGGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTGTTATGTTTGCTGGATTAAGGGCAATTa                                                                                                      >  1:894124/1‑143 (MQ=255)

                                    aaTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTa                                                                                                      >  1:858777/1‑143 (MQ=255)

                                     aTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAATGGTTGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTaa                                                                                                     >  1:980302/1‑143 (MQ=255)

                                      ttCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAAt                                                                                                    <  1:55148/143‑1 (MQ=255)

                                      ttCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGCTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAAt                                                                                                    <  1:210902/143‑1 (MQ=255)

                                         aaaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCtt                                                                                                 >  2:250479/1‑143 (MQ=255)

                                            gagGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGc                                                                                              <  1:487608/143‑1 (MQ=255)

                                              gggTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGGGCGCATATGACAGCAATGCAAAATTACGCCAACCTTAAGTTCTTAAGGTTGGCTGTGATGTTTGCTGGATTAAGGGGCATTAATCTTTGCtt                                                                                            >  1:883512/1‑143 (MQ=255)

                                               ggTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTa                                                                                           >  1:340221/1‑143 (MQ=255)

                                                 tCTTTTTAGTAGGCGCTTCAACATCACAAAGATGCCATCTCACAAAGTGCGCATATGCTCGCAAGGAAAACTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGAGAATTAATAATTGattatt                                                                                         >  2:863100/1‑143 (MQ=255)

                                                   tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCACAGCAAAATTAGGCCAAACTTAAGCTCTTAAGGTTGGCTTTTCTGCTTGCTGGATCAAGGGGAATTAATCTGCGCttcgtgc                                                                                       >  2:911576/1‑138 (MQ=255)

                                                   tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGTTTTAGGGTATTTAATCTTTGCTTATAgg                                                                                       >  1:256499/1‑143 (MQ=255)

                                                   tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTgg                                                                                       >  1:945580/1‑143 (MQ=255)

                                                   tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTgg                                                                                       >  2:563828/1‑143 (MQ=255)

                                                   tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTgg                                                                                       >  1:555211/1‑143 (MQ=255)

                                                      ttAGTAGGCGCTTCAACATCGCGGTGGTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGc                                                                                    <  2:155678/143‑1 (MQ=255)

                                                        agtagGCGCTTCCACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCAt                                                                                  <  2:334373/143‑1 (MQ=255)

                                                             gcgcTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGATCGAAAAGGAAAATGAAGCCAAGAATCGTGACTCATTTTTGGCTTTTATGTTTGCTGGATTCAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAg                                                                             >  1:228788/1‑143 (MQ=255)

                                                                cTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTCAAGTTCTTACGGCTGGCTTTTATGTTTCCTGGCTTACGGGGAATTAATCTTTGCTTACTGTTGCCTCTAAGGGa                                                                          >  2:237810/1‑143 (MQ=255)

                                                                 ttCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGAt                                                                         <  1:574459/143‑1 (MQ=255)

                                                                  tCAACATCACGGTGTTTCCATCGAACAACGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGCTCTTCAGCTTGGCTTTTATGTTTGCTGGATTAAGGGGACTTCATCTTTGCTTATTGGTGCATCTAAgagcga                                                                        >  1:353201/1‑138 (MQ=255)

                                                                    aaCATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTGTGCTTATTGGTGCATCTAAGGGATACg                                                                      >  1:56153/1‑143 (MQ=255)

                                                                     aCATCACGGTGTTTCCATCGAACCAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCATCTTTAAGTTCTTATGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACgg                                                                     <  2:410977/143‑1 (MQ=255)

                                                                      cATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACggg                                                                    <  2:858777/143‑1 (MQ=255)

                                                                        tCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTATTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGGATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGtg                                                                  >  1:697683/1‑142 (MQ=255)

                                                                         cACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGttt                                                                 <  2:318670/143‑1 (MQ=255)

                                                                           cGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCCTCTAAGGGATACGGGttttt                                                               >  1:694489/1‑143 (MQ=255)

                                                                            ggTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTCATCTTTGCTTCTTGGTGCATCTAAGGGATACGGGTTTTTa                                                              >  1:831463/1‑143 (MQ=255)

                                                                                tttCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTa                                                          <  2:826344/143‑1 (MQ=255)

                                                                                 ttCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGATGGCTGATATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATGGGTGCATCTAAGGGATACGGGTTTTTgtgtgg                                                         >  1:434024/1‑141 (MQ=255)

                                                                                   ccATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTGTTTGTGTAGcc                                                       >  1:465086/1‑143 (MQ=255)

                                                                                   ccATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATATATGCTGATTGGTGCATCTAAGGGATACGGGATTTTATGTAGcc                                                       >  1:930520/1‑143 (MQ=255)

                                                                                    cATCGAACACAGTGCGCATATGCTCGCAAAGCACAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCg                                                      <  1:952803/143‑1 (MQ=255)

                                                                                     aTCGAACCAAGGGCGCATATTCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTTTCAAGGTTTGTTTTTGGGTTTGCGGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCgg                                                     <  1:322810/143‑1 (MQ=255)

                                                                                      tCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGt                                                    >  2:801967/1‑143 (MQ=255)

                                                                                         aaaaaaGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGAGACGGGTTTTTATGTAGCCGGTTGt                                                 >  2:754204/4‑143 (MQ=255)

                                                                                         aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGt                                                 >  1:394821/1‑143 (MQ=255)

                                                                                         aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGt                                                 >  1:73279/1‑143 (MQ=255)

                                                                                         aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGATGt                                                 >  1:55162/1‑143 (MQ=255)

                                                                                         aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCAGCTAAGGGATACGGGTTTTTATGTAGCCGGTTGt                                                 >  2:841597/1‑143 (MQ=255)

                                                                                         aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATGAATCCTTGCTTATTGGTGCATCTAAGGGATGCGGGGTTGTATGTAGCCGGTTGt                                                 >  1:529815/1‑143 (MQ=255)

                                                                                          aCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTa                                                <  2:889072/143‑1 (MQ=255)

                                                                                          aCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTa                                                >  2:416816/1‑143 (MQ=255)

                                                                                                tGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAg                                          <  1:156481/143‑1 (MQ=255)

                                                                                                tGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTATTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCGTATTGGTGCATCTAAGGGATACGGGATGTTATGTAGCCGGATATAATTAAg                                          >  2:287402/1‑143 (MQ=255)

                                                                                                   gcATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTCGCCGGTTGTGATTCAgcgc                                       >  1:263482/1‑143 (MQ=255)

                                                                                                        tGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTGTTATGTTTGCTGGATTAAGGCGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCCGTTTTACTTAGGCGCacaaa                                  >  2:720084/1‑139 (MQ=255)

                                                                                                           tCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTCTATGTAGCCTGTTGTAATTCAGCGCATACATgg                               <  1:939576/143‑1 (MQ=255)

                                                                                                            cGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGc                              >  1:846558/1‑143 (MQ=255)

                                                                                                            cGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGACGGTTGTAATTCAGCGCATACGTGGc                              >  1:698496/1‑143 (MQ=255)

                                                                                                             gcaaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCg                             <  1:525026/143‑1 (MQ=255)

                                                                                                             gcaaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCg                             <  1:199317/143‑1 (MQ=255)

                                                                                                             gcaaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATAAGGGTTCTTATGTAGCCGGTTGTAATGCAGCGCACACATGACg                             >  2:500177/1‑143 (MQ=255)

                                                                                                                 agcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtga                         <  1:686108/143‑1 (MQ=255)

                                                                                                                 agcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTCCATTAGGGGGCATTAATCTATGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCAGGATGTCATTCAGCCCATACATGGCAGtga                         >  2:151830/1‑143 (MQ=255)

                                                                                                                  gcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTAAGTTTGCTGGATTAAGTGGCACTACTCTCGGCTTATTGGTGCATCTAAGGGGTACGGGATATTATGTAGCCGGGTGTACTTCAGCGCATACATGGCgaagat                        >  2:595153/1‑138 (MQ=255)

                                                                                                                   caaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGATTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatg                       >  2:747630/1‑143 (MQ=255)

                                                                                                                   caaaATTAAGCCAACCGTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatg                       <  1:578511/143‑1 (MQ=255)

                                                                                                                    aaaaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTCTATGTTTGCTGCACTAAGGGGAAATACTCTTAGCTTATTGGTGCATCTGCGGGGGTCGCGTGCATGTGTAGAAGAATGTCTTTCAGCGCATACATGGCGGtgatga                      <  2:654534/143‑1 (MQ=255)

                                                                                                                      aaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCGGTGCTTATTGGTGCATCTCAGGGATAGGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACGTGGCGGTGATGAcc                    >  2:248053/1‑143 (MQ=255)

                                                                                                                       aTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCa                   <  1:296079/143‑1 (MQ=255)

                                                                                                                         tAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCACCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATc                 >  1:249093/1‑143 (MQ=255)

                                                                                                                          aaGCCAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATAAGGGTTTTTATGTAGCCGGTTGGAATACAGCGTACACATGGGGGTGATGACCATct                >  2:880385/1‑142 (MQ=255)

                                                                                                                            gCCATCCTTAATTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGTAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAgg              <  1:399122/143‑1 (MQ=255)

                                                                                                                            gCCAAGCTTAAGTGCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAGTTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAgg              <  2:42455/143‑1 (MQ=255)

                                                                                                                             ccAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCACATACATGGCGGTGATGACCATCCggg             >  2:107888/1‑143 (MQ=255)

                                                                                                                             ccAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTCGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGGTGACCGTCAggg             >  2:814347/1‑143 (MQ=255)

                                                                                                                              cagccTTAAGTTCTTAAGGGTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGt            <  2:127942/140‑1 (MQ=255)

                                                                                                                              cAACCGTAAGGTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGt            <  2:634567/143‑1 (MQ=255)

                                                                                                                                 ccTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGGTTCAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGAc         >  1:638631/1‑143 (MQ=255)

                                                                                                                                 ccTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGCATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTGTGTAGCCGGTTGTAATTGCGCGCATACATGGCGGTGATGACCATCAGGGTgcc         >  1:443922/1‑141 (MQ=255)

                                                                                                                                     aaGTTGTTAAGGTGGGCTTTTAGGTTTGCTGGATTAAGGGGAATTGATCGTTGCTTATTGGTGCATCTAAGGGATCCGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAAt     <  2:289273/143‑1 (MQ=255)

                                                                                                                                        ttCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAc  >  1:686878/1‑143 (MQ=255)

AGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAC  >  NC_000913/4335518‑4335796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`