| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 756,792 | G→T | 100% | E296* (GAA→TAA) | sdhA → | succinate:quinone oxidoreductase, FAD binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 756,792 | 0 | G | T | 99.7% | 78.6 / ‑7.3 | 33 | E296* (GAA→TAA) | sdhA | succinate:quinone oxidoreductase, FAD binding protein |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base T (17/14); total (19/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTA > NC_000913/756653‑756912 | gggCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGGGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGGGGTTGCGCGTTCCATCCTGCTCGaaa > 1:640240/1‑143 (MQ=255) aCCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTcg > 1:403405/1‑143 (MQ=255) cGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCCGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTCAACGTcgcg > 2:180764/1‑143 (MQ=255) cGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTGTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGAGATCCAGCATGATCGAACACCGTACAGCTcgcg > 2:594474/1‑143 (MQ=255) gAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCgg > 2:772919/1‑143 (MQ=255) gTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCtgt > 1:733224/1‑143 (MQ=255) tGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACAGGGCGAACGTTTTATGGAGCGTTAGGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGa < 2:931834/143‑1 (MQ=255) tGCAGTGGTCAAGGCTGTCATCTGCTGCACAAACACGGCGATCGTTTTATGGTCCGTTATGCGCCGACCGCTAACGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGa < 1:385029/143‑1 (MQ=255) tgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCACGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGt > 2:609417/1‑143 (MQ=255) aGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGGTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGAGGGTCCGTgcg > 1:5321/1‑141 (MQ=255) cGGGTATCGGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGc < 1:966588/143‑1 (MQ=255) gTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAAACCGGGAAGGTAGCGGCTGTGATGGTCCGCGGGGGccc > 1:359169/1‑142 (MQ=255) gtgAACAAACTGGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaa < 2:516208/142‑1 (MQ=255) aaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactgaa > 1:249399/1‑143 (MQ=255) gCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGAt > 1:831486/1‑143 (MQ=255) gAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCCTGACCTGGTTGCGCCTACCATCATCAGCTAAATCCGCGAAGGTCGCGTCTGTGACGGTCCGTGGCGGCCACCCCCGAACCAGACACTccaaca > 2:946267/1‑138 (MQ=255) atcggtttCTGGCGCGTGATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTGGCGCGTTCCATCATGATCTAAATCCGTGAAGCTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCAc < 2:688253/138‑1 (MQ=255) gTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTg < 1:22093/143‑1 (MQ=255) aTGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTaa > 1:809290/1‑143 (MQ=255) ggAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaag < 1:349563/143‑1 (MQ=255) gCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaag > 2:764157/1‑143 (MQ=255) gcgcCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGGTCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGAGCGCCTGGGTGAGGAAGTTCTCGa > 2:775303/1‑143 (MQ=255) ggcgcaCGCCAAGCACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCGTCGTGGTCTAACCCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAAt < 2:344966/138‑1 (MQ=255) gCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTCAAGAAGTTCTCGGATCCCGTCt > 1:382829/1‑143 (MQ=255) aaaGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCCCCTGcc > 2:298510/1‑143 (MQ=255) gACCTGGCGGGGCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAGGGTCGCGGCTGTGATGGTCCGTGGGTGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCggg < 2:625764/143‑1 (MQ=255) tGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATc > 1:690040/1‑143 (MQ=255) cgggcgggGACGTGGTGGCGGGTCCCATCATGATCTAAAGCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTg < 1:476204/143‑1 (MQ=255) cGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTTGGGGTGACATGCGAGACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTg < 1:772919/143‑1 (MQ=255) gggCCGTGGCGGGGGTGCGCGTTGCATCGTGGTCTGGCTAGTCGAAGGGCGCGGCTGTGATGGGCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTgg < 2:434144/143‑1 (MQ=255) ggCCGTGACGTGGTTGCGCTTTCCATCATGATCTAAAGCCGTGGAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGa < 2:249399/143‑1 (MQ=255) tGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAAATGAAACTCGATCACCTGGGTAAGGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGt > 1:517325/1‑143 (MQ=255) ggTTGCGCGGTCCATCGTGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACGTACTCTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTa < 2:877112/143‑1 (MQ=255) | GGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTA > NC_000913/756653‑756912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |