Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	60.5 / NA	26	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/11);  total (15/11)

ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132649‑2132926                                                                                                                                           |                                                                                                                                            aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCGGGCTCTg                                                                                                                                         >  1:339881/1‑143 (MQ=255)       tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                   >  1:538675/1‑143 (MQ=255)          agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGTGTCGCctg                                                                                                                                >  2:104606/1‑142 (MQ=255)          agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctg                                                                                                                                >  1:474659/1‑142 (MQ=255)             gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGCATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATCCAGGCTCTGCTTCGCCtcttc                                                                                                                             >  1:175310/1‑143 (MQ=255)                     cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAGGTGGAAGTACCCGGAGAGTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATATGCGGGCTCTGCGGAGCGTCTTCTACGTTcc                                                                                                                     >  2:521249/1‑143 (MQ=255)                     cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTcc                                                                                                                     >  1:208718/1‑143 (MQ=255)                              tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc                                                                                                            <  2:199759/143‑1 (MQ=255)                               ttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                           <  2:168320/143‑1 (MQ=255)                                    ggCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGAAGAGGTGAACTGCATTTCGTCGTGATTGTTACTCAGGCTCTGCGGCGCCTATTCGACGTGCCAGAGTTCGCCGTACt                                                                                                      >  2:47711/1‑143 (MQ=255)                                         ggTCGAATTTGTCTTTGTGCTTGAGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTCTCCTACTTTCCACGGTTCGCCGTCCTccacc                                                                                                 >  2:267575/1‑143 (MQ=255)                                                 ttGCCTTCGTGCTTGCGGTAACAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCg                                                                                         <  1:141645/143‑1 (MQ=255)                                                                   gacgaGTGGAAGCACCGGGGGACGTTTTTCGGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCATCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                       <  2:630091/139‑1 (MQ=255)                                                                         tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGc                                                                 >  2:241017/1‑143 (MQ=255)                                                                                 cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         <  1:566170/143‑1 (MQ=255)                                                                                            ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                              <  1:481050/143‑1 (MQ=255)                                                                                             tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAtgcg                                             >  2:446949/1‑140 (MQ=255)                                                                                              tgcGGTGCCGAGGCGTTCTGCATATCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                            <  2:450760/141‑1 (MQ=255)                                                                                                    gACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaagca                                      >  1:353297/1‑143 (MQ=255)                                                                                                         ggTGATCTGCGTTGCTCCGTGATTGACAGGCGGGCTCTGCGTCGCCTCTTCGACTTTCCCCGGTTCGCCGTACTCCACCACCATCCTCCGGAAGATGTCGTAATCCTGTGCCGCTTGCAGTTCGGTATCGAACAAACACTCtt                                 <  2:381547/143‑1 (MQ=255)                                                                                                             aTCTGCTTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                             <  1:239311/143‑1 (MQ=255)                                                                                                              tCTGCATTTCACCGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                            <  2:314074/143‑1 (MQ=255)                                                                                                                 gCATTTCACCGTGATTTATTTTCAGGCTTTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACACCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                         <  1:30905/143‑1 (MQ=255)                                                                                                                 gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCAGCTTTCAGTTCGCGATCGACCAAACCACCTTTGCAActc                         >  2:494018/1‑141 (MQ=255)                                                                                                                                       cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt   >  1:300848/1‑143 (MQ=255)                                                                                                                                        aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATCTCGTAATCCTGTGCCGCTTTCAGCTCGGTATCGAACAAACACTCTTTGACACGCCATGCCCAGGTCAAGAccccccg  >  2:338198/1‑138 (MQ=255)                                                                                                                                           |                                                                                                                                            ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132649‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑