Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	94.9 / NA	31	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/16);  total (15/16)

TAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041274‑3041533                                                                                                                             |                                                                                                                                        tcgTCATTAATACGCCGTTACGCAACTCAGGCCTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcgc                                                                                                                       <  2:449092/141‑1 (MQ=255) tAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTGACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcgc                                                                                                                       <  1:115530/143‑1 (MQ=255)        tatgaCGCCGTGAACCCACTCTGGCCTCCATTGTGGCTTTTACTCCTTCTCCTCCCCTTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGc                                                                                                                <  2:714107/139‑1 (MQ=255)           tACGCCGTTAAGCAACTCAGGACTCTATGATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTGCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaa                                                                                                             <  1:603700/143‑1 (MQ=255)              gCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaaa                                                                                                          <  2:442752/143‑1 (MQ=255)              gCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCCCCATTACTGCCGGGGGCGGCGaaaaa                                                                                                          >  2:374190/1‑143 (MQ=255)                        aaCTCAGGGCTCTATCATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAgg                                                                                                <  2:416578/143‑1 (MQ=255)                          cTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCCCCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGt                                                                                              >  1:470099/1‑143 (MQ=255)                           tCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTc                                                                                             >  1:512423/1‑143 (MQ=255)                            cAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCa                                                                                            <  1:204516/143‑1 (MQ=255)                             agcgctcgATTATGGCTTTTACACCTGTGCCGCCCCGTCAGCCGACGGCTGCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTccg                                                                                           <  2:673920/139‑3 (MQ=255)                                   ctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATTACTGGGCGCGTGCCCCCATCACTTGCTCGGACCACTAAAAGTATATGCCGGGCCAGGGGAca                                                                                     >  2:427898/1‑143 (MQ=255)                                    tagtatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCGTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGAAGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcag                                                                                    <  2:11238/140‑1 (MQ=255)                                     attatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACCGCGAAACAGAGATGCAGGGTCAGGTGAcagc                                                                                   >  1:486686/1‑143 (MQ=255)                                         tGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGCCGCTGATGCCGCTTGATGACTGGGCGCGTGCCACCGTTACTGGCGCGGACAGCGAAACATACATGCCGGGTCCGGTGCCCGCCGAt                                                                               >  1:115936/1‑143 (MQ=255)                                            cTTTTACACCTTTCCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCG‑AAAATATATGCAGGGTCAGGTGACAGCAGATGTCa                                                                           <  1:439664/143‑1 (MQ=255)                                                 accccTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa                                                                       <  2:685547/140‑1 (MQ=255)                                                     cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTGCTGGCGCGGACAGCGAAAAACATATGCACGGTCAGGTGACAGCAGATGCCAGCCAGGTg                                                                   >  2:353990/1‑143 (MQ=255)                                                     cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAACTCTATGCAGGGCCAGGTGACCGCAGATGGCAGCCAGATg                                                                   >  2:418335/1‑143 (MQ=255)                                                        ttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                >  1:455890/1‑143 (MQ=255)                                                                cGTCAGCGTACGTCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCa                                                        <  1:690162/143‑1 (MQ=255)                                                                    tgcttACGGCTTCGGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCAc                                                    <  2:214280/139‑1 (MQ=255)                                                                      ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCt                                                  >  1:378172/1‑143 (MQ=255)                                                                           ggCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcg                                             >  2:428203/1‑143 (MQ=255)                                                                                       tttACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGCAGATCAGCACCTGCCCGCCGCCCATcgcg                                 >  2:636238/1‑143 (MQ=255)                                                                                        gtACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGa                                <  2:493819/142‑1 (MQ=255)                                                                                         tACCGCTGACGCTGATGCCGCTGGATGAGCGGGCGCGTGCCAGCATGACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAc                               <  2:399203/143‑1 (MQ=255)                                                                                                        tgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg                >  1:430156/1‑143 (MQ=255)                                                                                                            gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa            >  2:679329/1‑143 (MQ=255)                                                                                                                  tgaCTGGGCGCGTGCCACCAGTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt      <  1:450911/143‑1 (MQ=255)                                                                                                                      tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACg  >  2:674342/1‑143 (MQ=255)                                                                                                                             |                                                                                                                                        TAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041274‑3041533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑