Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	74.1 / NA	27	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/16);  total (11/16)

TTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGA  >  NC_000913/754307‑754574                                                                                                                                            |                                                                                                                                 ttCGCGGGAGGCGGTTGAGGTGAGGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGtt                                                                                                                               <  2:74689/143‑1 (MQ=255)        gATGCGGCTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCttt                                                                                                                        <  1:553484/143‑1 (MQ=255)             ggTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCACCTCGGAGGCTGGGTTTTACTTTTGTaa                                                                                                                   >  2:623017/1‑142 (MQ=255)                      gaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAttt                                                                                                          <  1:242955/143‑1 (MQ=255)                        gcgtggggTCAAAGGTGCATGGACCTGTTGAACCGAGAGTACGGATATCAATAACATCTTGACCGAGGGGGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                        <  1:717313/138‑1 (MQ=255)                        agCCTGGGTCACAGGTGACCGCACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGGACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                        <  1:97215/143‑1 (MQ=255)                                 cAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                               >  2:398707/1‑143 (MQ=255)                                  aaaGTTGAACCGACCTTTTGAACCGTGAGTACGGATATCAATAACATCTTGTCCCAGCGTGCCTTTCAGCACATCCTGTTCCACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCCCCCATTTAAGGTTTCCTTa                                                                                              <  2:592276/143‑1 (MQ=255)                                              acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTTTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                  <  1:464735/143‑1 (MQ=255)                                              acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                  <  1:143659/143‑1 (MQ=255)                                                cTTTTGAACCGAGAGTACGGATATCAATAACATCTTGATCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTTTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGt                                                                                <  1:176445/143‑1 (MQ=255)                                                cTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCTTTGAGGGTGGGATTTGCTTTTGTAACAGCCATTTAAGGTCTCCTTAGCTCCTTATTGCGt                                                                                >  1:289050/1‑143 (MQ=255)                                                cTTTTGAACCGAGAGTACGGATATCAATAACATCCTGACACAGCGTGCCTTTCAGCACATCCAGTTCAACGGCTGTATCCCCGTTGCGAGCGGGTTTTGCTTTTCTCTCAGCCATTTAAGCTCGCCTTAGCGCCTTATTACgc                                                                                >  2:29433/1‑142 (MQ=255)                                                                aCGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACtt                                                                >  1:616380/1‑143 (MQ=255)                                                                  ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCTATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                              <  1:656081/143‑1 (MQ=255)                                                                    atatCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGGTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTCGCGGAACTTAGAt                                                            >  2:556376/1‑143 (MQ=255)                                                                       tCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAtgtg                                                         >  2:366588/1‑143 (MQ=255)                                                                        cAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCATAGCGCCTTATTTCGTAAGACTGCCGGAACTTAAATTTGc                                                        >  1:23541/1‑143 (MQ=255)                                                                                 cTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcaca                                               >  2:558722/1‑143 (MQ=255)                                                                                               ccTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTa                                 >  2:277667/1‑143 (MQ=255)                                                                                                cTTTCAGCACCGCCCGTTCAACAGCTGTATCCCCGTTGAGGCTCGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAc                                <  2:59483/143‑1 (MQ=255)                                                                                                                ttCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGc                >  1:155929/1‑143 (MQ=255)                                                                                                                    aCAGCTGTCGCCCCGTTGAGGGTTGGTTTTGCTCTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCTGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc            <  2:392228/143‑1 (MQ=255)                                                                                                                     cAGCTGTATCGCCGTTGCGGGTGGGGTTTGCTGTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgcc           <  1:574622/143‑1 (MQ=255)                                                                                                                            atacgcGTTGGGGGCGGGTTTTGTTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt    <  1:711255/138‑1 (MQ=255)                                                                                                                             tCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgtg   <  1:398707/143‑1 (MQ=255)                                                                                                                              ccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGa  <  1:558722/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                 TTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGA  >  NC_000913/754307‑754574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑